本公开属于生物医药和疫苗技术领域，尤其涉及针对新型冠状病毒sars-cov-2的疫苗和组合物。
背景技术：
冠状病毒科(coronaviridae)病毒是一种包膜单股正链rna病毒，在rna病毒中拥有最大的基因组，可达27-32kb。冠状病毒科分为α、β、γ和δ4个属，其中α、β属易感于哺乳动物，而γ、δ属主要感染禽类。迄今为止，共发现了7种人类冠状病毒，如目前肆虐的新型冠状病毒(severeacuterespiratorysyndromecoronavirus2,sars-cov-2)。sars-cov-2具有高致病性，对公众健康造成严重威胁。自2020年初sars-cov-2引起的新型冠状病毒肺炎疫情席卷全球，截止到2020年9月，全球累积确诊病人超三千万，对全球公共卫生造成巨大威胁。目前尚无治疗新型冠状病毒肺炎的特效药，迫切需要有效的疫苗。
mrna疫苗是一种新型的疫苗，其将mrna传递至体内细胞，并在体内表达抗原蛋白，从而引起针对病原体感染的免疫应答，进而扩大机体的免疫能力。与传统疫苗相比，mrna疫苗最大的优点是开发和生产时间短，无传统疫苗的感染风险，无dna疫苗的基因组整合风险，在体内生产抗原更有效和便利，因此可以快速有效地应对突发的传染病疫情。
技术实现要素：
本公开通过在sars-cov-2病毒的刺突蛋白(s蛋白)的胞外结构域的c端氨基酸中进行一个或多个氨基酸残基突变为脯氨酸的突变，提供了具有s蛋白融合前稳定构象三聚体的重组s蛋白，并构建了编码上述重组蛋白的mrna。这些重组蛋白或其抗原性片段，以及编码它们的mrna如经过进一步优化的mrna，能够用于在受试者中诱导针对sars-cov-2病毒的免疫应答，从而预防和/或治疗与sars-cov-2感染相关的疾病或病症。
在第一方面，本公开提供了重组sars-cov-2刺突蛋白(s蛋白)或其抗原性片段，所述s蛋白或抗原性片段在胞外结构域的c端氨基酸区域中处具有一个或多个氨基酸残基突变为脯氨酸的突变。在一些实施方案中，所述c端氨基酸区域是指s蛋白的氨基酸位置817-987的区域，其中根据seqidno.1所示的氨基酸序列编号。
在一些实施方案中，所述突变可以包括k986p和/或v987p取代。例如，所述突变可以包括k986p和v987p取代两者。
sars-cov-2与sars-cov和mers-cov同属于冠状病毒科的β-冠状病毒属。sars-cov-2基因组序列全长29903bp，与sars-cov基因序列同一性为79.5％，与mers-cov序列同一性为40％。sars-cov-2病毒颗粒的主要结构包括单股正链核酸(ssrna)、刺突蛋白(spikeprotein，s)、膜蛋白(membraneprotein，m)、包膜蛋白(envelopprotein，e)和核衣壳蛋白(nucelocapsidprotein，n)。与其他β-冠状病毒类似，sars-cov-2病毒的对细胞的吸附入侵过程主要依赖于s蛋白；在该过程中，s蛋白以同源三聚体的形式装配，其具有短的胞质尾和疏水跨膜结构域，将蛋白质锚定到膜中。
如图1所示，s蛋白可分为受体结合亚基s1和膜融合亚基s2，s1亚基可进一步分成信号肽区域(signalpeptide，sp)、n-端区域(n-terminaldomain，ntd)和受体结合域(receptorbindingdomain，rbd)。s2亚基通过跨膜区锚定在膜上，它含有膜融合过程所需要的基本元件，包括：内在的膜融合肽(fusionpeptide，fp)、两个7肽重复序列(heptadrepeat，hr)、跨膜结构域(transmembranedomain，tm)，以及c末端的胞质结构域(cytoplasmicdomain，cp)。
通过对s蛋白预融合结构进行解析，发现s1亚基的rbd结构域经历铰链类似的构象移动来隐藏或暴露受体结合的关键位点，“向下”为受体不可结合状态，“向上”为受体可结合状态，处于较为不稳定的状态。这种构象使得s蛋白能够容易地与宿主受体血管紧张素转换酶2(ace2)结合。当rbd与受体结合后，s2亚基通过将fp插入宿主细胞膜而改变为融合后构象，hr1和hr2形成一个反平行的六螺旋束(6hb)，共同组成一个融合核心，最终导致病毒膜与细胞膜融合。使用冷冻电镜实验，在预融合构象中确定了大量的三聚体s糖蛋白结构域，预融合s蛋白上存在大量中和抗体敏感表位，而融合后构象使仅存在于预融合构象上的中和敏感表位的暴露量降至最低。因此，优化的疫苗抗原将保留存在于s蛋白的预融合构象形式中的表位，并诱导能够抑制病毒融合的抗体。
在一些实施方案中，重组s蛋白或其抗原性片段可以不包含功能性融合肽结构域(fp结构域：氨基酸位置788-806，根据seqidno:1进行编号)。例如，重组s蛋白或其抗原性片段可以包含经突变的融合肽结构域，例如通过一个或多个氨基酸残基的取代、缺失、插入和/或添加，其导致融合肽结构域失去其天然功能，例如介导病毒与宿主细胞膜进行融合的功能。或者，在一些实施方案中，重组s蛋白或其抗原性片段可以不包含融合肽结构域。
通过在重组s蛋白或其抗原性片段中去除功能性融合肽结构域，能够提高预融合构象的稳定性，使得保留和暴露s蛋白的预融合构象上存在的大量中和抗体敏感表位。
在一些实施方案中，重组s蛋白或其抗原性片段中的s1/s2切割位点rrar(氨基酸位置682-685，根据seqidno:1进行编号)可以经突变以失去被蛋白酶例如弗林样(furin-like)蛋白酶和溶酶体蛋白酶切割的能力。在一些实施方案中，可以将重组s蛋白或其抗原性片段中的s1/s2切割位点rrar突变为ggsg。
在一些实施方案中，重组s蛋白或其抗原性片段中的s2切割位点kr(a氨基酸位置814-815，根据seqidno:1进行编号)可以经突变以失去被蛋白酶例如弗林样蛋白酶和溶酶体蛋白酶切割的能力。在一些实施方案中，可以将重组s蛋白或其抗原性片段中的s2切割位点kr突变为an。
sars-cov-2病毒在细胞内包装过程中，s蛋白可能会被蛋白酶例如弗林样蛋白酶或溶酶体蛋白切割，分泌处s1亚基和s2亚基呈非融合状态的s蛋白。通过对重组s蛋白中的切割位点例如s1/s2切割位点和/或s2切割位点进行突变，可以避免重新s蛋白被蛋白酶切割，进一步提高其稳定性。
在一些实施方案中，重组s蛋白或其抗原性片段还可以包含三聚体结构域，所述三聚体结构域当表达时促进所述重组s蛋白或其抗原性片段形成三聚体。如本文所用，“三聚体结构域”是指当表达时，能够自发或诱导形成三聚体的蛋白质或多肽结构域。本领域已知多种此类三聚体结构域。通过在重组s蛋白或其抗原性片段中包括三聚体结构域(例如通过构建融合蛋白)，能够促进重组s蛋白或其抗原性片段形成三聚体构象，和/或稳定化重组s蛋白或其抗原性片段的三聚体构象。
在一些实施方案中，重组s蛋白或其抗原性片段中所述三聚体结构域可以包含t4噬菌体fibritin三聚体基序。在一些实施方案中，所述t4噬菌体fibritin三聚体基序可以具有seqidno.40所示的氨基酸序列。
在一些实施方案中，三聚体结构域可以与重组s蛋白或其抗原性片段直接融合。在另一些实施方案中，三聚体结构域可以通过接头与重组s蛋白或其抗原性片段融合。在一些实施方案中，三聚体结构域可以与重组s蛋白或其抗原性片段的n端融合。在另一些实施方案中，三聚体结构域可以与重组s蛋白或其抗原性片段的c端融合。例如，三聚体结构域可以通过接头与重组s蛋白或其抗原性片段的c端融合。
在一些实施方案中，所述接头序列可以包含saig。
在一些实施方案中，重组s蛋白或其抗原性片段还可以包含信号序列。在一些实施方案中，所述信号序列包含免疫球蛋白重链可变区(igvh)信号序列。例如，所述信号序列可以具有seqidno.39所示的氨基酸序列。
在第二方面，本公开提供了重组sars-cov-2刺突蛋白(s蛋白)或其抗原性片段，其具有选自seqidno.3-38和seqidno.49-52中任一项所示的氨基酸序列，或由选自seqidno:3-38和seqidno.49-52中任一项所述的氨基酸序列组成。
在优选的实施方案中，重组s蛋白或其抗原性片段可以具有选自seqidno.29、seqidno.32、seqidno.35、seqidno.38和seqidno.49-52中任一项所示的氨基酸序列，或由seqidno.29、seqidno.32、seqidno.35、seqidno.38和seqidno.49-52中任一项所示的氨基酸序列组成。
在第三方面，本公开提供了mrna，其编码本公开第一方面或第二方面的重组s蛋白或其抗原性片段。
在一些实施方案中，mrna从上游到下游可以包含帽结构、5’-utr、编码重组蛋白或其抗原性片段的开放阅读框(orf)、3’-utr和poly-a尾。
在一些实施方案中，所述帽结构可以具有m7g5’ppp5’(2’-ome)npg，其中m7g为n7-甲基鸟嘌呤核苷；p为磷酸；ppp为三磷酸；2’-ome为2’-甲氧基修饰；n为任意核苷，例如腺嘌呤核苷(a)、鸟嘌呤核苷(g)、胞嘧啶核苷(c)和尿嘧啶核苷(u)，或天然存在的其它核苷或经修饰的核苷。
在一些实施方案中，所述5’-utr可以包含选自下组的基因的5’-utr或其同源物、片段或变体：β-珠蛋白(hbb)基因、热休克蛋白70(hsp70)基因、轴丝动力蛋白重链2(dnah2)基因、羟基类固醇(17-β)脱氢酶(hsd17b4)基因。例如，所述变体序列可以与相应基因的野生型5’-utr序列具有至少80％，至少85％，至少90％，至少95％，至少98％，或至少99％的序列同一性。
在一些实施方案中，所述5’-utr可以包含kozak序列。
在一些实施方案中，所述5’-utr可以包含seqidno:41，其衍生自hsd17b4基因的5’-utr序列，或seqidno:42(kozak序列)，或seqidno:41和seqidno:42的组合。
在一些实施方案中，所述3’-utr可以包含选自下组的基因的3’-utr或其同源物、片段或变体：白蛋白(alb)基因、α-珠蛋白基因、β-珠蛋白(hbb)基因、酪氨酸羟化酶基因、热休克蛋白70(hsp70)基因，脂氧合酶基因和胶原蛋白α基因。例如，所述变体序列可以与相应基因的野生型3’-utr序列具有至少80％，至少85％，至少90％，至少95％，至少98％，或至少99％的序列同一性。
在一些实施方案中，所述3’-utr可以包含seqidno.43，其衍生自白蛋白(alb)基因的3’-utr序列。
在一些实施方案中，所述polya尾的长度可以为100-200个核苷酸，例如约100个核苷酸，约110个核苷酸，约120个核苷酸，约130个核苷酸，约140个核苷酸，约150个核苷酸，约160个核苷酸，约170个核苷酸，约180个核苷酸，约190个核苷酸，或约200个核苷酸。在一些实施方案中，所述polya尾的长度可以为约120个核苷酸。
在第四方面，本公开提供了mrna，其包含选自seqidno.44-47中任一项所示的序列，或由选自seqidno:44-47中任一项所示的序列组成。
在本公开的第三方面和第四方面的一些实施方案中，所述mrna中的一个或多个核苷酸可以是经修饰的。例如，所述mrna中的一个或多个核苷酸(例如所有核苷酸)可以各自独立替换为天然存在的核苷酸类似物或人工合成的核苷酸类似物。
在一些实施方案中，所述天然存在的核苷酸类似物可以选自假尿苷(pseudouridine)、2-硫尿苷(2-thiouridine)、5-甲基尿苷(5-methyluridine)，5-甲基胞苷(5-methylcytidine)和n6-甲基腺苷(n6-methyladenosine)。
在一些实施方案中，所述人工合成的核苷酸类似物可以选自n1-甲基假尿苷(n1-methylpseudouridine)和5-乙炔基尿苷(5-ethynyluridine)。
在一些实施方案中，所述mrna中的一个或多个尿苷三磷酸(utp)例如所有utp可以各自独立替换为假尿苷三磷酸(pseudo-utp)、1-甲基-假尿苷三磷酸(n1-methyl-pseudo-utp)或5-乙炔基尿苷三磷酸(5-ethynyl-utp)。
在一些实施方案中，所述mrna中的一个或多个胞苷三磷酸(ctp)例如所有ctp可以替换为5-甲基胞苷三磷酸(5-methyl-ctp)。
在第五方面，本公开提供了核酸分子，其编码如本公开第三方面或第四方面所述的mrna。所述核酸分子例如可以是载体例如质粒载体或病毒载体的形式。在一些实施方案中，所述核酸分子可以用于在体外通过转录制备本公开的mrna。
在第六方面，本公开提供了mrna-载体颗粒，其包含本公开第三方面或第四方面所述的mrna和包封所述mrna的载体材料。
在一些实施方案中，所述载体材料可以选自鱼精蛋白、脂质纳米颗粒(lnp)、高分子材料和无机纳米颗粒。在优选的实施方案中，所述载体材料是lnp。
在一些实施方案中，所述lnp可以包含离子化脂质、聚乙二醇化脂质、胆固醇及衍生物和磷脂中的一种或多种。例如，所述lnp可以包含离子化脂质、聚乙二醇化脂质、胆固醇及衍生物和磷脂中的任一种，其中任意两种、任意三种或全部四种的组合。在一些实施方案中，所述lnp可以包含离子化脂质、聚乙二醇化脂质、胆固醇及衍生物和磷脂。
在第七方面，本公开提供了组合物，其包含一种或多种如本公开第一方面和第二方面中定义的重组s蛋白或其抗原性片段、本公开第三方面和第四方面中定义的mrna或本公开第六方面中定义的mrna-载体颗粒，以及任选的一种或多种药学上可接受的载体、赋形剂或稀释剂。
如本文所用，“药学上可接受的”是指在合理的医学判断的范围内适合用于与人和动物的组织接触而没有过度毒性、刺激性、变应性应答或其它问题或并发症，与合理的益处/风险比相称的载体、赋形剂或稀释剂。
在一些实施方案中，所述组合物可以是疫苗组合物，任选的所述疫苗组合物可以包含一种或多种佐剂。
在一些实施方案中，所述组合物或疫苗组合物还包含一种或多种另外的治疗剂。例如，所述治疗剂可以选自另外的抗原性蛋白或多肽、抗体、激素或激素类似物，和小分子药物。
在第八方面，本公开提供了本公开第一方面和第二方面中定义的重组s蛋白或其抗原性片段、本公开第三方面和第四方面中定义的mrna、本公开第六方面中定义的mrna-载体颗粒或本公开第七方面中定义的组合物(例如疫苗组合物)在制备用于在受试者中预防和/或治疗与sars-cov-2感染相关的疾病或病症的药物中的用途。
还提供了在受试者中预防和/或治疗与sars-cov-2感染相关的疾病或病症的方法，其包括将本公开第一方面和第二方面中定义的重组s蛋白或其抗原性片段、本公开第三方面和第四方面中定义的mrna、本公开第六方面中定义的mrna-载体颗粒或本公开第七方面中定义的组合物(例如疫苗组合物)施用于所述受试者的步骤。
还提供了本公开第一方面和第二方面中定义的重组s蛋白或其抗原性片段、本公开第三方面和第四方面中定义的mrna、本公开第六方面中定义的mrna-载体颗粒或本公开第七方面中定义的组合物(例如疫苗组合物)，其用于在受试者中预防和/或治疗与sars-cov-2感染相关的疾病或病症。
在第九方面，本公开提供了制备mrna疫苗的方法，其包括：
a.将离子化脂质、聚乙二醇化脂质、胆固醇及衍生物和磷脂溶解在乙醇溶液中；
b.通过微流控混合器将所述脂质乙醇溶液与mrna水溶液混合得到脂质纳米颗粒(lnp)；
c.分离和纯化步骤b中获得的lnp从而获得所述mrna疫苗。
在一些实施方案中，所述mrna如本公开第三方面和第四方面中定义。
在一些实施方案中，步骤c包括对步骤b中获得的混合物进行透析、超滤和微米膜过滤。
附图说明
图1显示了sars-cov-2的s蛋白s1亚基和s2亚基的各个结构域的示意图(上图)。还显示了s蛋白在病毒与宿主细胞膜融合前和融合后的构象(下图)。
图2显示了实施例3中具有seqidno:44-48的核苷酸序列的mrna的电泳结果图，其中采用agilent2200tapestation全自动电泳系统检测mrna大小及完整性。
图3显示了实施例4中检测从mrna表达的野生型s蛋白(图3a)重组s蛋白(图3b)的蛋白印迹结果图。
图4显示了实施例5中，mrna在动物体内模型中诱导的t淋巴细胞γ干扰素分泌的结果图。
图5显示了实施例6中，具有不同修饰的mrna在动物体内模型中诱导的t淋巴细胞γ干扰素分泌(图5a)和s蛋白特异性igg抗体(图5b)的结果图。
图6显示了实施例7中免疫接种后小鼠血清igg抗体滴度的结果。
图7显示了实施例8中免疫接种后小鼠体内总igg抗体持久度的结果图。
图8显示了实施例9中不同品系小鼠免疫接种后体内总igg抗体滴度的结果图。
图9显示了实施例11中s蛋白mrna疫苗诱导th1型免疫应答的结果图。**指示p<0.01,***指示p<0.001。
图10显示了实施例12中mrna疫苗大鼠急性毒性试验体重变化的结果图。
图11显示了实施例12中mrna疫苗大鼠急性毒性试验摄食量变化的结果图。
图12显示了实施例13中非变性蛋白印迹以检测s蛋白三聚体的结果图。
具体实施方式
下面结合具体实施例来进一步描述本发明，本发明的优点和特点将会随着描述而更为清楚。但这些实施例仅是范例性的，并不对本发明的范围构成任何限制。本领域技术人员应该理解的是，在不偏离本发明的精神和范围下可以对本发明技术方案的细节和形式进行修改或替换，但这些修改和替换均落入本发明的保护范围内。
实施例1.mrna序列的优化
通过dnaworks(v3.2.4)在线软件对sars-cov-2s蛋白(seqidno.1)的编码序列进行序列优化，包括：针对在人体内表达时的密码子偏好性进行调整、常用密码子的使用频率进行调整、提高序列的gc含量等。优化得到核苷酸序列如seqidno.2所示，该核苷酸序列使得转录后的mrna结构更稳定，在哺乳动物和人体内的目标蛋白翻译效率更高。
实施例2.表达预融合稳定形式的sars-cov-2s三聚体蛋白
s蛋白由胞外结构域(ecd)、跨膜结构域(tm)和c末端的胞浆区域(cp)组成。其中，胞外结构域可进一步分成信号肽区域(sp)、n-端区域(ntd)、受体结合域(rbd)、一个内在的膜融合肽(fp)和两个7肽重复序列(hr)，属于第一类病毒膜融合蛋白。s蛋白的结构域示意图如图1所示，其中预融合结构是s蛋白的功能构象，而融合后大量仅存于融合前形式的敏感中和表位被掩蔽。表达预融合稳定形式的sars-cov-2s三聚体蛋白，是开发安全有效的sars-cov-2疫苗的关键。
在本实施例中，采用了以下策略表达预融合稳定形式的sars-cov-2s三聚体蛋白。
(1)编码区编码s蛋白的胞外结构域(ecd；氨基酸1-1213)，并在蛋白c端序列中引入了1个脯氨酸或2个脯氨酸突变如k986p和/或v987p突变。其中优选2个脯氨酸突变，两个脯氨酸残基的取代大大提高了预融合构象的稳定性。
(2)s2亚基通过融合肽结构域(fp)插入宿主细胞膜而转变为融合后构象。因此，在(1)基础上去除融合肽结构域fp(氨基酸788-806)以进一步提高预融合构象的稳定性。
(3)编码区编码膜锚定的s蛋白(氨基酸1-1237)，去除c末端的胞质区域cp(氨基酸1238-1273)，并在所得的蛋白c端引入1个脯氨酸或2个脯氨酸突变如k986p和/或v987p突变。
(4)在(3)的基础上去除融合肽结构域fp(氨基酸788-806)。
(5)在(1)-(4)的基础上，为了避免sars-cov-2s蛋白在细胞内包装过程中被furin样蛋白酶或溶酶体蛋白酶切割分泌出s1和s2呈非融合状态的s蛋白，对切割位点氨基酸序列进行突变。将s1/s2切割位点rrar(氨基酸682-685)突变为ggsg。
(6)在(5)的基础上，将s2切割位点kr(氨基酸814-815)突变为an。突变s2切割位点进一步提高s蛋白稳定性。
(7)在(1)-(6)的基础上，分泌信号肽片段(aa1-13)可以是免疫球蛋白重链可变区igvh信号序列(seqidno.39)。
(8)在(1)-(6)的基础上，s蛋白c末端通过接头序列融合三聚体结构域形成稳定三聚体s蛋白，例如通过接头序列saig与t4噬菌体fibritin三聚体基序(seqidno.40)融合。
通过以上修饰，得到了预融合稳定形式的重组sars-cov-2s蛋白，其具有如seqidno.3-38和seqidno.49-52所示的氨基酸序列。
实施例3.mrna制备
通过体外转录制备编码预融合稳定形式的sars-cov-2病毒s蛋白的mrna。s蛋白编码区核酸序列通过全基因合成方法获得，通过常规分子克隆方法将编码区核酸序列与质粒载体相连接，得到用于体外转录的dna质粒模板。
表1.转录起始位点
质粒载体包含的元件包括t7启动子序列(taatacgactcactata)，起始核酸序列可以是agg/ggg/cgg中的任一种，如表1所示；5’-utr序列(seqidno.41)，3’-utr序列(seqidno.43)，120nt3’端聚腺苷酸(polya)序列，在3’端聚腺苷酸序列后包含xbal酶切位点(tctaga)。
转录得到的mrna序列如seqidno.44，seqidno.45，seqidno.46，seqidno.47，seqidno.48所示，其分别编码具有实施例2中所述的seqidno.49、seqidno.50、seqidno.51、seqidno.52的重组s蛋白以及野生型s蛋白(seqidno.1)。使用agilent2200tapestation全自动电泳系统分析具有上述序列的mrna的大小和完整性。结果如图2所示，转录mrna条带单一，无明显降解。
实施例4.重组s蛋白表达检测
使用2.5μg编码预融合稳定形式s蛋白的mrna(seqidno.44，seqidno.45，seqidno.46，seqidno.47)和野生型s蛋白的mrna(seqidno.48)分别转染293t细胞,未转染mrna的细胞作为阴性对照。转染24小时后，通过蛋白免疫印迹检测s蛋白表达，其中使用兔抗sars冠状病毒刺突s1亚基蛋白抗体(义翘神州cat#40150-rp01)作为一抗，和羊抗兔-hrp二抗进行标记。结果如图3所示，野生型s蛋白在细胞中表达被剪切(图3a)，而预融合s蛋白更加稳定，只检测到单一蛋白条带(图3b)。
实施例5.不同mrna序列的免疫原性研究
如实施例3中所述制备具有seqidno.44-47所示的编码预融合稳定s蛋白的mrna序列。使用上述4种mrna序列分别制备疫苗，其中使用包括以下组分的脂质纳米颗粒包封mrna:8-(3-羟基丙基)(9,12-二烯基-十八烷基-1)-胺基-辛酸十七烷-9-醇酯、二硬脂酰基磷脂酰胆碱(dspc)、1,2-二肉豆蔻酰-rac-甘油-3-甲氧基聚乙二醇2000(dmg-peg2000)和胆固醇。制备方法包括将上述组分溶解在乙醇溶液中，通过微流控混合器将所述脂质乙醇溶液与mrna水溶液混合得到脂质纳米颗粒，以及对混合物进行透析、超滤和微米膜过滤从而获得mrna的lnp疫苗制剂。
将所得4种疫苗用于balb/c小鼠免疫试验。取6-8周龄的雌性balb/c小鼠，每组9只小鼠，通过肌肉注射分别于第0天和第14天接种两次疫苗制剂以进行初次和加强免疫，剂量为10μg，注射体积为50μl。在第一次免疫7天后，取小鼠脾脏分离脾脏淋巴细胞，通过elispot法检测分泌γ干扰素的t淋巴细胞，结果如图4所示。结果表明，使用上述4种mrna制备的疫苗均能够在balb/c小鼠体内诱导细胞免疫应答，其中具有序列seqidno.46的mrna诱导了的细胞免疫应答更强。
在第二次免疫14天后，通过间接elisa法检测小鼠血清中的s蛋白特异性igg抗体。通过拟合量效曲线计算igg抗体ec50滴度(表2)。结果表明，4种mrna均能够在balb/c小鼠体内诱导产生高滴度的igg抗体。
表2.不同mrna序列疫苗诱导产生igg抗体ec50滴度
实施例6.不同化学修饰的mrna疫苗的免疫原性
在本实施例中，为了进一步开发稳定的和低天然免疫反应的mrna，制备了化学修饰核苷的mrna。将经修饰的mrna(mrna序列如seqidno.45所示)制备疫苗，具体修饰方式如表3所示。
表3.针对seqidno.45的修饰
将所得3种mrna疫苗制剂用于balb/c小鼠免疫试验。取6-8周龄balb/c小鼠，雌性，每组9只小鼠，通过肌肉注射接种疫苗制剂，注射体积50μl。分别于第0天和第14天进行初次和加强免疫。修饰1、修饰2和修饰3的免疫剂量为4μg和50μg。
初次免疫7天后，取小鼠脾脏分离脾脏淋巴细胞，通过elispot法检测分泌γ干扰素的t淋巴细胞，结果如图5a所示，3种修饰均在小鼠体内有效诱导了细胞免疫应答。加强免疫14天后，通过间接elisa法检测混合血清s蛋白特异性igg抗体滴度。结果如图5b所示，3种经修饰的mrna制备的疫苗制剂均在小鼠体内诱导产生高水平的igg抗体。
实施例7.s蛋白mrna疫苗诱导产生高滴度的igg抗体
应用实施例6中修饰1的mrna制备得到sars-cov2mrna疫苗(mrna序列如seqidno.45所示)。制备的疫苗制剂测定浓度，用于balb/c小鼠免疫试实验。balb/c小鼠(6只/组)分别于第0天(初次)和第14天(加强)经肌肉注射接种mrna疫苗，剂量为4μg和50μg，给药体积50μl，阴性对照为等体积pbs溶液。在加强免疫28天后采集小鼠血清，用elisa法检测小鼠体内产生的特异性igg抗体水平。
结果如图6所示，小鼠体内产生的igg抗体呈剂量依赖性增加，其中高剂量组igg滴度高达1.6x107。
实施例8.s蛋白mrna疫苗免疫持久性
应用实施例6中修饰1的mrna制备得到sars-cov2mrna疫苗(mrna序列如seqidno.45所示)。制备的疫苗制剂测定浓度，用于balb/c小鼠免疫试验。balb/c小鼠(4只/组)分别于第0天(初次)和第14天(加强)经肌肉注射接种mrna疫苗，剂量为4μg和50μg，体积50μl，阴性对照为等体积pbs溶液。于加强免疫后1个月和3个月采集小鼠血清，用elisa法检测小鼠体内的特异性igg抗体水平。
结果如图7所示，加强免疫3个月后小鼠体内igg抗体滴度无明显降低，表明s蛋白mrna疫苗能够诱导持久性免疫应答。
实施例9.不同品系小鼠免疫接种后产生高滴度的igg抗体
应用实施例6中修饰1的mrna制备得到sars-cov2mrna疫苗(mrna序列如seqidno.45所示)。制备的疫苗制剂测定浓度，用于balb/c小鼠、c57bl/6j小鼠、b6c3f1小鼠免疫接种试验。每组9只小鼠，分别经肌肉注射单次接种mrna疫苗，剂量为1μg、5μg和20μg，体积50μl，阴性对照为等体积pbs溶液。在接种后14天采集小鼠血清，将每组9只小鼠的血清混合，用elisa法检测小鼠体内产生的特异性igg抗体。
结果如图8所示。单次接种sars-cov2mrna疫苗即可在所有三种小鼠体内产生高滴度的特异性igg抗体。单次接种高剂量mrna疫苗在balb/c和b6c3f1小鼠产生的抗体滴度达到1x105。
实施例10.s蛋白mrna疫苗诱导产生中和抗体
应用实施例6中编号为修饰1的mrna制备得到sars-cov2mrna疫苗(mrna序列如seqidno.45所示)。制备的疫苗制剂测定浓度，用于balb/c小鼠免疫试验。balb/c小鼠(4只/组)分别于第0天(初次)和第14天(加强)经肌肉注射接种mrna疫苗，剂量为4μg和50μg，给药体积50μl，阴性对照为等体积pbs溶液。于加强免疫后28天采集小鼠血清，通过假病毒中和试验进行s蛋白特异性中和抗体检测。具体操作如下：
1.将血清于56℃水浴灭活30min，6000g离心3min，将上清转移至1.5ml离心管。
2.用含双抗的dmem细胞完全培养基作稀释液倍比稀释血清。
3.用dmem完全培养基将假病毒稀释至1.3×104tcid50/ml。
4.每孔加入50μl稀释的血清和50μl假病毒(650tcid50/孔)，于37℃共孵育1小时。
5.向96孔板中每孔加100μl细胞，使每孔细胞为2×104个。对照设置假病毒对照孔、血清毒性对照孔和正常细胞空白对照孔。
6.将96孔板放入细胞培养箱中，于37℃、5％co2培养20-28小时。
7.培养完成后，从细胞培养箱中取出96孔板，吸弃150μl上清，然后加入100μl荧光素酶检测试剂，检测luciferase化学发光值。
8.计算中和抑制率：抑制率＝[1－(样品组的发光强度均值－空白对照发光强度均值)/(病毒对照组的发光强度均值－空白对照发光强度均值)]×100％。
9.根据中和抑制率结果，利用reed-muench法计算假病毒中和ic50。
中和抗体检测结果如表4所示。其中低剂量和高剂量免疫小鼠均产生中和抗体，高剂量组产生较高滴度的中和抗体。
表4.中和抗体检测结果
实施例11.s蛋白mrna疫苗诱导th1型免疫应答
th1/th2细胞亚群及其相互之间的平衡在免疫应答的调节中起着关键作用。据报道sars疫苗诱导的th2型免疫应答与攻毒后产生增强肺部病变有关，而th1型免疫应答产生保护作用。
应用实施例6中编号为修饰1的mrna制备得到编码sars-cov2s蛋白胞外域三聚体mrna疫苗(seqidno.45)。制备的疫苗制剂测定浓度，用于balb/c小鼠免疫试验。balb/c小鼠(9只/组)分别于第0天(初次)和第14天(加强)经肌肉注射mrna疫苗，剂量为1μg、5μg和20μg，给药体积50μl，阴性对照为等体积pbs溶液。初次免疫7天后，取小鼠脾脏分离脾脏淋巴细胞，通过elispot法检测th1型细胞因子infγ、il2和th2型细胞因子il4、il5的水平。检测il-5和il2具体操作按照mabtechmouseil-5/il2elispotplus(hrp)试剂盒说明书进行，检测infγ和il4具体操作按照达优mouseifn-γ/il4elispot试剂盒说明书进行。加强免疫14天后采集小鼠血清，用elisa法检测小鼠体内产生的特异性igg1和igg2a抗体滴度。
结果如图9a所示，疫苗接种后再次用s蛋白抗原刺激脾脏淋巴细胞，分泌th1型细胞因子infγ、il2的t淋巴细胞显著多于分泌th2型细胞因子il4、il5的t淋巴细胞。igg抗体亚型检测结果如图9b所示，小鼠体内产生的igg2a抗体滴度高于igg1抗体。细胞因子及igg亚型抗体检测结果表明，mrna疫苗诱导的免疫反应为th1型偏倚的免疫反应。
实施例12.mrna疫苗安全性的安全性评价
取8只wistar大鼠，雌雄各半，体重相差不超过10％，随机分为溶剂对照组和受试物mrna疫苗组(mrna浓度为2mg/ml)。每只动物单日给药3次，每次注射250μl，给药时间间隔4h，左右腿交替给药。总给药量为每只1.50mg，相当于人每单位体重最大剂量的1200倍(假设人最大给药剂量是0.25mg)。
临床观察：给药后24h内，每小时观察1次，给药后24-72h，每6小时观察1次，给药后4-14天，每天观察1次，详细记录毒性反应症状，症状出现和消失的时间，死亡时间(如有发生)。给药后每天记录1次体重，给药后每2天记录1次摄食量。于给药后第14天将剩余全部动物称量体重后安乐死并剖检取主要脏器：心、肝、脾、肺、肾、胸腺、淋巴结称重，计算脏器系数。解剖后取心、肝、脾、肺、肾、肠道、胸腺、淋巴结、注射部位肌肉组织及其他观察到有病理学改变的器官保存于固定液中，通过h&e染色考查各组织器官的病理学变化，病变程度评分参照表5。
表5.病变严重程度评分标准
实验动物的体重变化结果如图10所示，溶剂对照组雄性大鼠体重持续增长，受试物mrna疫苗组大鼠体重初期降低，至第7天恢复至给药前水平，后续持续增长。摄食量变化结果如图11所示，溶剂对照组单只大鼠24h内摄食量稳定在18-35g之间；受试物mrna疫苗组大鼠初期摄食量减少，至第6-7天恢复至正常水平。脏器系数结果如表6所示，其显示了雄性大鼠和雌性大鼠合并后的数据。与溶剂对照组比较，给药组无明显差异。组织的病理学变化结果如表7所示，与溶剂对照组大鼠比较，受试物mrna疫苗组大鼠的心脏、肝脏、肾脏、脾脏、胸腺、淋巴结无病理改变。
综合上述结果，在1200倍大剂量注射mrna疫苗后大鼠仅在肺部和腿部有轻微的病理改变。
表6.mrna疫苗大鼠急性毒性试验脏器系数结果
表7.mrna疫苗大鼠急性毒性试验组织病理学结果
“—”表示无异常。
实施例13.s抗原蛋白的三聚体结构
按照实施例3所述的方法制备编码融合flag标签的预融合稳定s蛋白三聚体的mrna(mrna序列为seqidno.45，其编码具有seqidno.45所示的氨基酸序列的重组s蛋白)和野生型s蛋白mrna，然后利用ribofecttmmrna转染试剂盒通过反向转染方法转染hek293t细胞。采用非变性的方式制备蛋白样品，然后用非变性蛋白印迹检测三聚体蛋白。
非变性的蛋白印迹检测结果如图12所示，编码预融合稳定s蛋白三聚体的mrna表达的s蛋白与野生型s蛋白相比，野生型s蛋白在140kda附近出现了一条强条带为s蛋白单体；s-trimer在140kda处没有条带，而在泳道顶端有一条强条带，表明预融合稳定s蛋白mrna表达的s抗原蛋白形成了稳定的三聚体结构。
序列表
<110>广州市锐博生物科技有限公司
阿格纳生物制药有限公司
<120>基于sars-cov-2的s抗原蛋白的疫苗和组合物
<130>c20p7644
<160>52
<170>patentinversion3.5
<210>1
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565570575
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580585590
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595600605
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610615620
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625630635640
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645650655
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705710715720
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740745750
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755760765
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770775780
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785790795800
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805810815
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885890895
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900905910
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thrglnglnleuileargalaalagluileargalaseralaasn
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leualaalathrlysmetserglucysvalleuglyglnserlys
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<223>优化的sars-cov-2s蛋白编码序列
<400>2
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aatcctgtgctgcctttcaatgacggcgtgtacttcgccagcaccgagaagagcaatatc300
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aacaacgctacaaatgtggtgatcaaggtgtgcgagttccagttctgcaatgaccctttc420
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ggagctgcggcatattacgtgggctacctgcagcctagaaccttcctgctgaagtacaat840
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gtgatcagaggcgacgaggtgagacagatcgcgccagggcagaccggcaagatcgccgac1260
tacaattacaagctgcctgacgacttcaccggctgcgtgatcgcgtggaactctaacaac1320
ctggactcgaaagttggaggcaattacaattacctgtacagactgttcagaaagagcaat1380
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aatggcgtggagggcttcaattgctacttccctctgcagagctacggcttccagcctacc1500
aatggcgtgggctaccagccttacagagtggtggtgctgagcttcgagctgctgcacgct1560
cccgctaccgtgtgcggccctaagaagagcaccaatctggtgaagaataagtgcgtgaat1620
ttcaatttcaatggtctaactggaacgggcgtgctgaccgagagcaataagaagtttctt1680
ccctttcaacaattcggcagagacatcgccgacaccacagatgctgtaagagaccctcag1740
accctggagatcctggacatcactccgtgtagcttcggcggcgtgagcgtgatcacaccg1800
ggcaccaataccagcaatcaggtggccgtgctgtaccaggacgtgaattgcaccgaggtg1860
cctgtggccatccacgccgaccagctgactcccacttggagggtatattccacgggaagc1920
aatgtgttccagaccagagccggctgcctgatcggcgccgagcacgtgaataatagctac1980
gagtgcgacatccctatcggcgccggcatctgcgccagctaccagacccagaccaatagc2040
cctagaagagccagaagcgtggccagccagagcatcatcgcctacaccatgagcctgggc2100
gccgagaatagcgtggcctacagcaataatagcatcgccatccctaccaatttcaccatc2160
agcgtgaccaccgaaatattaccagtctccatgaccaagaccagcgtggactgcaccatg2220
tacatctgcggcgacagcaccgagtgcagcaatctgctgctgcagtacggcagcttctgc2280
acccagctgaatagagccctgaccggcatcgccgtggagcaggacaagaatacccaggag2340
gtgttcgcccaggtgaagcagatctacaagactccgccgatcaaggacttcggcggcttc2400
aatttcagccaaatactcccagatccaagcaagcctagcaagaggagcttcatcgaggac2460
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ctacctcctctgctgaccgacgagatgatagcacaatatacgtcggcattgctcgctggc2640
acgatcacatcgggctggactttcggcgccggagcagcgttgcaaatccctttcgccatg2700
cagatggcctacagattcaatggcatcggcgtgacccagaatgtgctgtacgagaatcag2760
aagctgatcgccaatcagttcaatagcgccatcggcaagatccaggacagcctgagcagc2820
accgccagcgccctgggcaagctgcaggacgtggtgaatcagaatgcccaggccctgaat2880
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ctgagcagactggacaaggtggaggccgaggtgcaaattgatcgtcttattactggcaga3000
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agcgccaatctggccgccaccaagatgagcgagtgcgtgctgggccagagcaagagagtg3120
gacttctgcggcaagggctaccacctgatgagcttccctcagagcgctccacatggcgtg3180
gtgttcctgcacgtgacctacgtgcctgcccaggagaagaatttcaccaccgcacccgca3240
atctgccacgacggcaaggcccacttccctagagagggcgtgttcgtgagcaatggcacc3300
cactggttcgtgacccagagaaatttctacgagcctcagatcatcaccaccgacaatacc3360
ttcgtgagcggcaattgcgacgtggtgatcgggatagtcaataatactgtctacgaccct3420
ctgcagcctgagctggacagcttcaaggaggagctggacaagtacttcaagaatcacacc3480
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aaggaaattgatcggctcaacgaagtggccaagaatctgaatgagagcctgatcgacctg3600
caggagctgggcaagtacgagcagtacatcaagtggccttggtacatctggctgggcttc3660
atcgccggcctgatcgccatcgtgatggtgaccatcatgctgtgctgcatgacctcctgt3720
tgttcctgtttgaaagggtgttgttcgtgtgggtcctgctgcaagttcgacgaggacgac3780
agcgagcctgtgctgaagggcgtgaagctgcactacacctaa3822
<210>3
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<213>人工序列
<220>
<223>重组sars-cov-2s蛋白
<400>3
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151015
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202530
thrargglyvaltyrtyrproasplysvalpheargserservalleu
354045
hisserthrglnaspleupheleuprophepheserasnvalthrtrp
505560
phehisalailehisvalserglythrasnglythrlysargpheasp
65707580
asnprovalleupropheasnaspglyvaltyrphealaserthrglu
859095
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100105110
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115120125
lysvalcysglupheglnphecysasnasppropheleuglyvaltyr
130135140
tyrhislysasnasnlyssertrpmetgluserglupheargvaltyr
145150155160
serseralaasnasncysthrpheglutyrvalserglnpropheleu
165170175
metaspleugluglylysglnglyasnphelysasnleuarggluphe
180185190
valphelysasnileaspglytyrphelysiletyrserlyshisthr
195200205
proileasnleuvalargaspleuproglnglypheseralaleuglu
210215220
proleuvalaspleuproileglyileasnilethrargpheglnthr
225230235240
leuleualaleuhisargsertyrleuthrproglyaspserserser
245250255
glytrpthralaglyalaalaalatyrtyrvalglytyrleuglnpro
260265270
argthrpheleuleulystyrasngluasnglythrilethraspala
275280285
valaspcysalaleuaspproleusergluthrlyscysthrleulys
290295300
serphethrvalglulysglyiletyrglnthrserasnpheargval
305310315320
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325330335
propheglygluvalpheasnalathrargphealaservaltyrala
340345350
trpasnarglysargileserasncysvalalaasptyrservalleu
355360365
tyrasnseralaserpheserthrphelyscystyrglyvalserpro
370375380
thrlysleuasnaspleucysphethrasnvaltyralaaspserphe
385390395400
valileargglyaspgluvalargglnilealaproglyglnthrgly
405410415
lysilealaasptyrasntyrlysleuproaspaspphethrglycys
420425430
valilealatrpasnserasnasnleuaspserlysvalglyglyasn
435440445
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450455460
gluargaspileserthrgluiletyrglnalaglyserthrprocys
465470475480
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485490495
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500505510
leuserphegluleuleuhisalaproalathrvalcysglyprolys
515520525
lysserthrasnleuvallysasnlyscysvalasnpheasnpheasn
530535540
glyleuthrglythrglyvalleuthrgluserasnlyslyspheleu
545550555560
propheglnglnpheglyargaspilealaaspthrthraspalaval
565570575
argaspproglnthrleugluileleuaspilethrprocysserphe
580585590
glyglyvalservalilethrproglythrasnthrserasnglnval
595600605
alavalleutyrglnaspvalasncysthrgluvalprovalalaile
610615620
hisalaaspglnleuthrprothrtrpargvaltyrserthrglyser
625630635640
asnvalpheglnthrargalaglycysleuileglyalagluhisval
645650655
asnasnsertyrglucysaspileproileglyalaglyilecysala
660665670
sertyrglnthrglnthrasnserproargargalaargservalala
675680685
serglnserileilealatyrthrmetserleuglyalagluasnser
690695700
valalatyrserasnasnserilealaileprothrasnphethrile
705710715720
servalthrthrgluileleuprovalsermetthrlysthrserval
725730735
aspcysthrmettyrilecysglyaspserthrglucysserasnleu
740745750
leuleuglntyrglyserphecysthrglnleuasnargalaleuthr
755760765
glyilealavalgluglnasplysasnthrglngluvalphealagln
770775780
vallysglniletyrlysthrproproilelysasppheglyglyphe
785790795800
asnpheserglnileleuproaspproserlysproserlysargser
805810815
pheilegluaspleuleupheasnlysvalthrleualaaspalagly
820825830
pheilelysglntyrglyaspcysleuglyaspilealaalaargasp
835840845
leuilecysalaglnlyspheasnglyleuthrvalleuproproleu
850855860
leuthraspglumetilealaglntyrthrseralaleuleualagly
865870875880
thrilethrserglytrpthrpheglyalaglyalaalaleuglnile
885890895
prophealametglnmetalatyrargpheasnglyileglyvalthr
900905910
glnasnvalleutyrgluasnglnlysleuilealaasnglnpheasn
915920925
seralaileglylysileglnaspserleuserserthralaserala
930935940
leuglylysleuglnaspvalvalasnglnasnalaglnalaleuasn
945950955960
thrleuvallysglnleuserserasnpheglyalaileserserval
965970975
leuasnaspileleuserargleuaspprovalglualagluvalgln
980985990
ileaspargleuilethrglyargleuglnserleuglnthrtyrval
99510001005
thrglnglnleuileargalaalagluileargalaseralaasn
101010151020
leualaalathrlysmetserglucysvalleuglyglnserlys
102510301035
argvalaspphecysglylysglytyrhisleumetserphepro
104010451050
glnseralaprohisglyvalvalpheleuhisvalthrtyrval
105510601065
proalaglnglulysasnphethrthralaproalailecyshis
107010751080
aspglylysalahispheproarggluglyvalphevalserasn
108510901095
glythrhistrpphevalthrglnargasnphetyrgluprogln
110011051110
ileilethrthraspasnthrphevalserglyasncysaspval
111511201125
valileglyilevalasnasnthrvaltyraspproleuglnpro
113011351140
gluleuaspserphelysglugluleuasplystyrphelysasn
114511501155
histhrserproaspvalaspleuglyaspileserglyileasn
116011651170
alaservalvalasnileglnlysgluileaspargleuasnglu
117511801185
valalalysasnleuasngluserleuileaspleuglngluleu
119011951200
glylystyrgluglntyrilelystrppro
12051210
<210>4
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<213>人工序列
<220>
<223>重组sars-cov-2s蛋白
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metphevalpheleuvalleuleuproleuvalserserglncysval
151015
asnleuthrthrargthrglnleuproproalatyrthrasnserphe
202530
thrargglyvaltyrtyrproasplysvalpheargserservalleu
354045
hisserthrglnaspleupheleuprophepheserasnvalthrtrp
505560
phehisalailehisvalserglythrasnglythrlysargpheasp
65707580
asnprovalleupropheasnaspglyvaltyrphealaserthrglu
859095
lysserasnileileargglytrpilepheglythrthrleuaspser
100105110
lysthrglnserleuleuilevalasnasnalathrasnvalvalile
115120125
lysvalcysglupheglnphecysasnasppropheleuglyvaltyr
130135140
tyrhislysasnasnlyssertrpmetgluserglupheargvaltyr
145150155160
serseralaasnasncysthrpheglutyrvalserglnpropheleu
165170175
metaspleugluglylysglnglyasnphelysasnleuarggluphe
180185190
valphelysasnileaspglytyrphelysiletyrserlyshisthr
195200205
proileasnleuvalargaspleuproglnglypheseralaleuglu
210215220
proleuvalaspleuproileglyileasnilethrargpheglnthr
225230235240
leuleualaleuhisargsertyrleuthrproglyaspserserser
245250255
glytrpthralaglyalaalaalatyrtyrvalglytyrleuglnpro
260265270
argthrpheleuleulystyrasngluasnglythrilethraspala
275280285
valaspcysalaleuaspproleusergluthrlyscysthrleulys
290295300
serphethrvalglulysglyiletyrglnthrserasnpheargval
305310315320
glnprothrgluserilevalargpheproasnilethrasnleucys
325330335
propheglygluvalpheasnalathrargphealaservaltyrala
340345350
trpasnarglysargileserasncysvalalaasptyrservalleu
355360365
tyrasnseralaserpheserthrphelyscystyrglyvalserpro
370375380
thrlysleuasnaspleucysphethrasnvaltyralaaspserphe
385390395400
valileargglyaspgluvalargglnilealaproglyglnthrgly
405410415
lysilealaasptyrasntyrlysleuproaspaspphethrglycys
420425430
valilealatrpasnserasnasnleuaspserlysvalglyglyasn
435440445
tyrasntyrleutyrargleuphearglysserasnleulysprophe
450455460
gluargaspileserthrgluiletyrglnalaglyserthrprocys
465470475480
asnglyvalgluglypheasncystyrpheproleuglnsertyrgly
485490495
pheglnprothrasnglyvalglytyrglnprotyrargvalvalval
500505510
leuserphegluleuleuhisalaproalathrvalcysglyprolys
515520525
lysserthrasnleuvallysasnlyscysvalasnpheasnpheasn
530535540
glyleuthrglythrglyvalleuthrgluserasnlyslyspheleu
545550555560
propheglnglnpheglyargaspilealaaspthrthraspalaval
565570575
argaspproglnthrleugluileleuaspilethrprocysserphe
580585590
glyglyvalservalilethrproglythrasnthrserasnglnval
595600605
alavalleutyrglnaspvalasncysthrgluvalprovalalaile
610615620
hisalaaspglnleuthrprothrtrpargvaltyrserthrglyser
625630635640
asnvalpheglnthrargalaglycysleuileglyalagluhisval
645650655
asnasnsertyrglucysaspileproileglyalaglyilecysala
660665670
sertyrglnthrglnthrasnserproargargalaargservalala
675680685
serglnserileilealatyrthrmetserleuglyalagluasnser
690695700
valalatyrserasnasnserilealaileprothrasnphethrile
705710715720
servalthrthrgluileleuprovalsermetthrlysthrserval
725730735
aspcysthrmettyrilecysglyaspserthrglucysserasnleu
740745750
leuleuglntyrglyserphecysthrglnleuasnargalaleuthr
755760765
glyilealavalgluglnasplysasnthrglngluvalphealagln
770775780
vallysglniletyrlysthrproproilelysasppheglyglyphe
785790795800
asnpheserglnileleuproaspproserlysproserlysargser
805810815
pheilegluaspleuleupheasnlysvalthrleualaaspalagly
820825830
pheilelysglntyrglyaspcysleuglyaspilealaalaargasp
835840845
leuilecysalaglnlyspheasnglyleuthrvalleuproproleu
850855860
leuthraspglumetilealaglntyrthrseralaleuleualagly
865870875880
thrilethrserglytrpthrpheglyalaglyalaalaleuglnile
885890895
prophealametglnmetalatyrargpheasnglyileglyvalthr
900905910
glnasnvalleutyrgluasnglnlysleuilealaasnglnpheasn
915920925
seralaileglylysileglnaspserleuserserthralaserala
930935940
leuglylysleuglnaspvalvalasnglnasnalaglnalaleuasn
945950955960
thrleuvallysglnleuserserasnpheglyalaileserserval
965970975
leuasnaspileleuserargleuasplysproglualagluvalgln
980985990
ileaspargleuilethrglyargleuglnserleuglnthrtyrval
99510001005
thrglnglnleuileargalaalagluileargalaseralaasn
101010151020
leualaalathrlysmetserglucysvalleuglyglnserlys
102510301035
argvalaspphecysglylysglytyrhisleumetserphepro
104010451050
glnseralaprohisglyvalvalpheleuhisvalthrtyrval
105510601065
proalaglnglulysasnphethrthralaproalailecyshis
107010751080
aspglylysalahispheproarggluglyvalphevalserasn
108510901095
glythrhistrpphevalthrglnargasnphetyrgluprogln
110011051110
ileilethrthraspasnthrphevalserglyasncysaspval
111511201125
valileglyilevalasnasnthrvaltyraspproleuglnpro
113011351140
gluleuaspserphelysglugluleuasplystyrphelysasn
114511501155
histhrserproaspvalaspleuglyaspileserglyileasn
116011651170
alaservalvalasnileglnlysgluileaspargleuasnglu
117511801185
valalalysasnleuasngluserleuileaspleuglngluleu
119011951200
glylystyrgluglntyrilelystrppro
12051210
<210>5
<211>1213
<212>prt
<213>人工序列
<220>
<223>重组sars-cov-2s蛋白
<400>5
metphevalpheleuvalleuleuproleuvalserserglncysval
151015
asnleuthrthrargthrglnleuproproalatyrthrasnserphe
202530
thrargglyvaltyrtyrproasplysvalpheargserservalleu
354045
hisserthrglnaspleupheleuprophepheserasnvalthrtrp
505560
phehisalailehisvalserglythrasnglythrlysargpheasp
65707580
asnprovalleupropheasnaspglyvaltyrphealaserthrglu
859095
lysserasnileileargglytrpilepheglythrthrleuaspser
100105110
lysthrglnserleuleuilevalasnasnalathrasnvalvalile
115120125
lysvalcysglupheglnphecysasnasppropheleuglyvaltyr
130135140
tyrhislysasnasnlyssertrpmetgluserglupheargvaltyr
145150155160
serseralaasnasncysthrpheglutyrvalserglnpropheleu
165170175
metaspleugluglylysglnglyasnphelysasnleuarggluphe
180185190
valphelysasnileaspglytyrphelysiletyrserlyshisthr
195200205
proileasnleuvalargaspleuproglnglypheseralaleuglu
210215220
proleuvalaspleuproileglyileasnilethrargpheglnthr
225230235240
leuleualaleuhisargsertyrleuthrproglyaspserserser
245250255
glytrpthralaglyalaalaalatyrtyrvalglytyrleuglnpro
260265270
argthrpheleuleulystyrasngluasnglythrilethraspala
275280285
valaspcysalaleuaspproleusergluthrlyscysthrleulys
290295300
serphethrvalglulysglyiletyrglnthrserasnpheargval
305310315320
glnprothrgluserilevalargpheproasnilethrasnleucys
325330335
propheglygluvalpheasnalathrargphealaservaltyrala
340345350
trpasnarglysargileserasncysvalalaasptyrservalleu
355360365
tyrasnseralaserpheserthrphelyscystyrglyvalserpro
370375380
thrlysleuasnaspleucysphethrasnvaltyralaaspserphe
385390395400
valileargglyaspgluvalargglnilealaproglyglnthrgly
405410415
lysilealaasptyrasntyrlysleuproaspaspphethrglycys
420425430
valilealatrpasnserasnasnleuaspserlysvalglyglyasn
435440445
tyrasntyrleutyrargleuphearglysserasnleulysprophe
450455460
gluargaspileserthrgluiletyrglnalaglyserthrprocys
465470475480
asnglyvalgluglypheasncystyrpheproleuglnsertyrgly
485490495
pheglnprothrasnglyvalglytyrglnprotyrargvalvalval
500505510
leuserphegluleuleuhisalaproalathrvalcysglyprolys
515520525
lysserthrasnleuvallysasnlyscysvalasnpheasnpheasn
530535540
glyleuthrglythrglyvalleuthrgluserasnlyslyspheleu
545550555560
propheglnglnpheglyargaspilealaaspthrthraspalaval
565570575
argaspproglnthrleugluileleuaspilethrprocysserphe
580585590
glyglyvalservalilethrproglythrasnthrserasnglnval
595600605
alavalleutyrglnaspvalasncysthrgluvalprovalalaile
610615620
hisalaaspglnleuthrprothrtrpargvaltyrserthrglyser
625630635640
asnvalpheglnthrargalaglycysleuileglyalagluhisval
645650655
asnasnsertyrglucysaspileproileglyalaglyilecysala
660665670
sertyrglnthrglnthrasnserproargargalaargservalala
675680685
serglnserileilealatyrthrmetserleuglyalagluasnser
690695700
valalatyrserasnasnserilealaileprothrasnphethrile
705710715720
servalthrthrgluileleuprovalsermetthrlysthrserval
725730735
aspcysthrmettyrilecysglyaspserthrglucysserasnleu
740745750
leuleuglntyrglyserphecysthrglnleuasnargalaleuthr
755760765
glyilealavalgluglnasplysasnthrglngluvalphealagln
770775780
vallysglniletyrlysthrproproilelysasppheglyglyphe
785790795800
asnpheserglnileleuproaspproserlysproserlysargser
805810815
pheilegluaspleuleupheasnlysvalthrleualaaspalagly
820825830
pheilelysglntyrglyaspcysleuglyaspilealaalaargasp
835840845
leuilecysalaglnlyspheasnglyleuthrvalleuproproleu
850855860
leuthraspglumetilealaglntyrthrseralaleuleualagly
865870875880
thrilethrserglytrpthrpheglyalaglyalaalaleuglnile
885890895
prophealametglnmetalatyrargpheasnglyileglyvalthr
900905910
glnasnvalleutyrgluasnglnlysleuilealaasnglnpheasn
915920925
seralaileglylysileglnaspserleuserserthralaserala
930935940
leuglylysleuglnaspvalvalasnglnasnalaglnalaleuasn
945950955960
thrleuvallysglnleuserserasnpheglyalaileserserval
965970975
leuasnaspileleuserargleuaspproproglualagluvalgln
980985990
ileaspargleuilethrglyargleuglnserleuglnthrtyrval
99510001005
thrglnglnleuileargalaalagluileargalaseralaasn
101010151020
leualaalathrlysmetserglucysvalleuglyglnserlys
102510301035
argvalaspphecysglylysglytyrhisleumetserphepro
104010451050
glnseralaprohisglyvalvalpheleuhisvalthrtyrval
105510601065
proalaglnglulysasnphethrthralaproalailecyshis
107010751080
aspglylysalahispheproarggluglyvalphevalserasn
108510901095
glythrhistrpphevalthrglnargasnphetyrgluprogln
110011051110
ileilethrthraspasnthrphevalserglyasncysaspval
111511201125
valileglyilevalasnasnthrvaltyraspproleuglnpro
113011351140
gluleuaspserphelysglugluleuasplystyrphelysasn
114511501155
histhrserproaspvalaspleuglyaspileserglyileasn
116011651170
alaservalvalasnileglnlysgluileaspargleuasnglu
117511801185
valalalysasnleuasngluserleuileaspleuglngluleu
119011951200
glylystyrgluglntyrilelystrppro
12051210
<210>6
<211>1194
<212>prt
<213>人工序列
<220>
<223>重组sars-cov-2s蛋白
<400>6
metphevalpheleuvalleuleuproleuvalserserglncysval
151015
asnleuthrthrargthrglnleuproproalatyrthrasnserphe
202530
thrargglyvaltyrtyrproasplysvalpheargserservalleu
354045
hisserthrglnaspleupheleuprophepheserasnvalthrtrp
505560
phehisalailehisvalserglythrasnglythrlysargpheasp
65707580
asnprovalleupropheasnaspglyvaltyrphealaserthrglu
859095
lysserasnileileargglytrpilepheglythrthrleuaspser
100105110
lysthrglnserleuleuilevalasnasnalathrasnvalvalile
115120125
lysvalcysglupheglnphecysasnasppropheleuglyvaltyr
130135140
tyrhislysasnasnlyssertrpmetgluserglupheargvaltyr
145150155160
serseralaasnasncysthrpheglutyrvalserglnpropheleu
165170175
metaspleugluglylysglnglyasnphelysasnleuarggluphe
180185190
valphelysasnileaspglytyrphelysiletyrserlyshisthr
195200205
proileasnleuvalargaspleuproglnglypheseralaleuglu
210215220
proleuvalaspleuproileglyileasnilethrargpheglnthr
225230235240
leuleualaleuhisargsertyrleuthrproglyaspserserser
245250255
glytrpthralaglyalaalaalatyrtyrvalglytyrleuglnpro
260265270
argthrpheleuleulystyrasngluasnglythrilethraspala
275280285
valaspcysalaleuaspproleusergluthrlyscysthrleulys
290295300
serphethrvalglulysglyiletyrglnthrserasnpheargval
305310315320
glnprothrgluserilevalargpheproasnilethrasnleucys
325330335
propheglygluvalpheasnalathrargphealaservaltyrala
340345350
trpasnarglysargileserasncysvalalaasptyrservalleu
355360365
tyrasnseralaserpheserthrphelyscystyrglyvalserpro
370375380
thrlysleuasnaspleucysphethrasnvaltyralaaspserphe
385390395400
valileargglyaspgluvalargglnilealaproglyglnthrgly
405410415
lysilealaasptyrasntyrlysleuproaspaspphethrglycys
420425430
valilealatrpasnserasnasnleuaspserlysvalglyglyasn
435440445
tyrasntyrleutyrargleuphearglysserasnleulysprophe
450455460
gluargaspileserthrgluiletyrglnalaglyserthrprocys
465470475480
asnglyvalgluglypheasncystyrpheproleuglnsertyrgly
485490495
pheglnprothrasnglyvalglytyrglnprotyrargvalvalval
500505510
leuserphegluleuleuhisalaproalathrvalcysglyprolys
515520525
lysserthrasnleuvallysasnlyscysvalasnpheasnpheasn
530535540
glyleuthrglythrglyvalleuthrgluserasnlyslyspheleu
545550555560
propheglnglnpheglyargaspilealaaspthrthraspalaval
565570575
argaspproglnthrleugluileleuaspilethrprocysserphe
580585590
glyglyvalservalilethrproglythrasnthrserasnglnval
595600605
alavalleutyrglnaspvalasncysthrgluvalprovalalaile
610615620
hisalaaspglnleuthrprothrtrpargvaltyrserthrglyser
625630635640
asnvalpheglnthrargalaglycysleuileglyalagluhisval
645650655
asnasnsertyrglucysaspileproileglyalaglyilecysala
660665670
sertyrglnthrglnthrasnserproargargalaargservalala
675680685
serglnserileilealatyrthrmetserleuglyalagluasnser
690695700
valalatyrserasnasnserilealaileprothrasnphethrile
705710715720
servalthrthrgluileleuprovalsermetthrlysthrserval
725730735
aspcysthrmettyrilecysglyaspserthrglucysserasnleu
740745750
leuleuglntyrglyserphecysthrglnleuasnargalaleuthr
755760765
glyilealavalgluglnasplysasnthrglngluvalphealagln
770775780
vallysglnproaspproserlysproserlysargserpheileglu
785790795800
aspleuleupheasnlysvalthrleualaaspalaglypheilelys
805810815
glntyrglyaspcysleuglyaspilealaalaargaspleuilecys
820825830
alaglnlyspheasnglyleuthrvalleuproproleuleuthrasp
835840845
glumetilealaglntyrthrseralaleuleualaglythrilethr
850855860
serglytrpthrpheglyalaglyalaalaleuglnilepropheala
865870875880
metglnmetalatyrargpheasnglyileglyvalthrglnasnval
885890895
leutyrgluasnglnlysleuilealaasnglnpheasnseralaile
900905910
glylysileglnaspserleuserserthralaseralaleuglylys
915920925
leuglnaspvalvalasnglnasnalaglnalaleuasnthrleuval
930935940
lysglnleuserserasnpheglyalaileserservalleuasnasp
945950955960
ileleuserargleuaspprovalglualagluvalglnileasparg
965970975
leuilethrglyargleuglnserleuglnthrtyrvalthrglngln
980985990
leuileargalaalagluileargalaseralaasnleualaalathr
99510001005
lysmetserglucysvalleuglyglnserlysargvalaspphe
101010151020
cysglylysglytyrhisleumetserpheproglnseralapro
102510301035
hisglyvalvalpheleuhisvalthrtyrvalproalaglnglu
104010451050
lysasnphethrthralaproalailecyshisaspglylysala
105510601065
hispheproarggluglyvalphevalserasnglythrhistrp
107010751080
phevalthrglnargasnphetyrgluproglnileilethrthr
108510901095
aspasnthrphevalserglyasncysaspvalvalileglyile
110011051110
valasnasnthrvaltyraspproleuglnprogluleuaspser
111511201125
phelysglugluleuasplystyrphelysasnhisthrserpro
113011351140
aspvalaspleuglyaspileserglyileasnalaservalval
114511501155
asnileglnlysgluileaspargleuasngluvalalalysasn
116011651170
leuasngluserleuileaspleuglngluleuglylystyrglu
117511801185
glntyrilelystrppro
1190
<210>7
<211>1194
<212>prt
<213>人工序列
<220>
<223>重组sars-cov-2s蛋白
<400>7
metphevalpheleuvalleuleuproleuvalserserglncysval
151015
asnleuthrthrargthrglnleuproproalatyrthrasnserphe
202530
thrargglyvaltyrtyrproasplysvalpheargserservalleu
354045
hisserthrglnaspleupheleuprophepheserasnvalthrtrp
505560
phehisalailehisvalserglythrasnglythrlysargpheasp
65707580
asnprovalleupropheasnaspglyvaltyrphealaserthrglu
859095
lysserasnileileargglytrpilepheglythrthrleuaspser
100105110
lysthrglnserleuleuilevalasnasnalathrasnvalvalile
115120125
lysvalcysglupheglnphecysasnasppropheleuglyvaltyr
130135140
tyrhislysasnasnlyssertrpmetgluserglupheargvaltyr
145150155160
serseralaasnasncysthrpheglutyrvalserglnpropheleu
165170175
metaspleugluglylysglnglyasnphelysasnleuarggluphe
180185190
valphelysasnileaspglytyrphelysiletyrserlyshisthr
195200205
proileasnleuvalargaspleuproglnglypheseralaleuglu
210215220
proleuvalaspleuproileglyileasnilethrargpheglnthr
225230235240
leuleualaleuhisargsertyrleuthrproglyaspserserser
245250255
glytrpthralaglyalaalaalatyrtyrvalglytyrleuglnpro
260265270
argthrpheleuleulystyrasngluasnglythrilethraspala
275280285
valaspcysalaleuaspproleusergluthrlyscysthrleulys
290295300
serphethrvalglulysglyiletyrglnthrserasnpheargval
305310315320
glnprothrgluserilevalargpheproasnilethrasnleucys
325330335
propheglygluvalpheasnalathrargphealaservaltyrala
340345350
trpasnarglysargileserasncysvalalaasptyrservalleu
355360365
tyrasnseralaserpheserthrphelyscystyrglyvalserpro
370375380
thrlysleuasnaspleucysphethrasnvaltyralaaspserphe
385390395400
valileargglyaspgluvalargglnilealaproglyglnthrgly
405410415
lysilealaasptyrasntyrlysleuproaspaspphethrglycys
420425430
valilealatrpasnserasnasnleuaspserlysvalglyglyasn
435440445
tyrasntyrleutyrargleuphearglysserasnleulysprophe
450455460
gluargaspileserthrgluiletyrglnalaglyserthrprocys
465470475480
asnglyvalgluglypheasncystyrpheproleuglnsertyrgly
485490495
pheglnprothrasnglyvalglytyrglnprotyrargvalvalval
500505510
leuserphegluleuleuhisalaproalathrvalcysglyprolys
515520525
lysserthrasnleuvallysasnlyscysvalasnpheasnpheasn
530535540
glyleuthrglythrglyvalleuthrgluserasnlyslyspheleu
545550555560
propheglnglnpheglyargaspilealaaspthrthraspalaval
565570575
argaspproglnthrleugluileleuaspilethrprocysserphe
580585590
glyglyvalservalilethrproglythrasnthrserasnglnval
595600605
alavalleutyrglnaspvalasncysthrgluvalprovalalaile
610615620
hisalaaspglnleuthrprothrtrpargvaltyrserthrglyser
625630635640
asnvalpheglnthrargalaglycysleuileglyalagluhisval
645650655
asnasnsertyrglucysaspileproileglyalaglyilecysala
660665670
sertyrglnthrglnthrasnserproargargalaargservalala
675680685
serglnserileilealatyrthrmetserleuglyalagluasnser
690695700
valalatyrserasnasnserilealaileprothrasnphethrile
705710715720
servalthrthrgluileleuprovalsermetthrlysthrserval
725730735
aspcysthrmettyrilecysglyaspserthrglucysserasnleu
740745750
leuleuglntyrglyserphecysthrglnleuasnargalaleuthr
755760765
glyilealavalgluglnasplysasnthrglngluvalphealagln
770775780
vallysglnproaspproserlysproserlysargserpheileglu
785790795800
aspleuleupheasnlysvalthrleualaaspalaglypheilelys
805810815
glntyrglyaspcysleuglyaspilealaalaargaspleuilecys
820825830
alaglnlyspheasnglyleuthrvalleuproproleuleuthrasp
835840845
glumetilealaglntyrthrseralaleuleualaglythrilethr
850855860
serglytrpthrpheglyalaglyalaalaleuglnilepropheala
865870875880
metglnmetalatyrargpheasnglyileglyvalthrglnasnval
885890895
leutyrgluasnglnlysleuilealaasnglnpheasnseralaile
900905910
glylysileglnaspserleuserserthralaseralaleuglylys
915920925
leuglnaspvalvalasnglnasnalaglnalaleuasnthrleuval
930935940
lysglnleuserserasnpheglyalaileserservalleuasnasp
945950955960
ileleuserargleuasplysproglualagluvalglnileasparg
965970975
leuilethrglyargleuglnserleuglnthrtyrvalthrglngln
980985990
leuileargalaalagluileargalaseralaasnleualaalathr
99510001005
lysmetserglucysvalleuglyglnserlysargvalaspphe
101010151020
cysglylysglytyrhisleumetserpheproglnseralapro
102510301035
hisglyvalvalpheleuhisvalthrtyrvalproalaglnglu
104010451050
lysasnphethrthralaproalailecyshisaspglylysala
105510601065
hispheproarggluglyvalphevalserasnglythrhistrp
107010751080
phevalthrglnargasnphetyrgluproglnileilethrthr
108510901095
aspasnthrphevalserglyasncysaspvalvalileglyile
110011051110
valasnasnthrvaltyraspproleuglnprogluleuaspser
111511201125
phelysglugluleuasplystyrphelysasnhisthrserpro
113011351140
aspvalaspleuglyaspileserglyileasnalaservalval
114511501155
asnileglnlysgluileaspargleuasngluvalalalysasn
116011651170
leuasngluserleuileaspleuglngluleuglylystyrglu
117511801185
glntyrilelystrppro
1190
<210>8
<211>1194
<212>prt
<213>人工序列
<220>
<223>重组sars-cov-2s蛋白
<400>8
metphevalpheleuvalleuleuproleuvalserserglncysval
151015
asnleuthrthrargthrglnleuproproalatyrthrasnserphe
202530
thrargglyvaltyrtyrproasplysvalpheargserservalleu
354045
hisserthrglnaspleupheleuprophepheserasnvalthrtrp
505560
phehisalailehisvalserglythrasnglythrlysargpheasp
65707580
asnprovalleupropheasnaspglyvaltyrphealaserthrglu
859095
lysserasnileileargglytrpilepheglythrthrleuaspser
100105110
lysthrglnserleuleuilevalasnasnalathrasnvalvalile
115120125
lysvalcysglupheglnphecysasnasppropheleuglyvaltyr
130135140
tyrhislysasnasnlyssertrpmetgluserglupheargvaltyr
145150155160
serseralaasnasncysthrpheglutyrvalserglnpropheleu
165170175
metaspleugluglylysglnglyasnphelysasnleuarggluphe
180185190
valphelysasnileaspglytyrphelysiletyrserlyshisthr
195200205
proileasnleuvalargaspleuproglnglypheseralaleuglu
210215220
proleuvalaspleuproileglyileasnilethrargpheglnthr
225230235240
leuleualaleuhisargsertyrleuthrproglyaspserserser
245250255
glytrpthralaglyalaalaalatyrtyrvalglytyrleuglnpro
260265270
argthrpheleuleulystyrasngluasnglythrilethraspala
275280285
valaspcysalaleuaspproleusergluthrlyscysthrleulys
290295300
serphethrvalglulysglyiletyrglnthrserasnpheargval
305310315320
glnprothrgluserilevalargpheproasnilethrasnleucys
325330335
propheglygluvalpheasnalathrargphealaservaltyrala
340345350
trpasnarglysargileserasncysvalalaasptyrservalleu
355360365
tyrasnseralaserpheserthrphelyscystyrglyvalserpro
370375380
thrlysleuasnaspleucysphethrasnvaltyralaaspserphe
385390395400
valileargglyaspgluvalargglnilealaproglyglnthrgly
405410415
lysilealaasptyrasntyrlysleuproaspaspphethrglycys
420425430
valilealatrpasnserasnasnleuaspserlysvalglyglyasn
435440445
tyrasntyrleutyrargleuphearglysserasnleulysprophe
450455460
gluargaspileserthrgluiletyrglnalaglyserthrprocys
465470475480
asnglyvalgluglypheasncystyrpheproleuglnsertyrgly
485490495
pheglnprothrasnglyvalglytyrglnprotyrargvalvalval
500505510
leuserphegluleuleuhisalaproalathrvalcysglyprolys
515520525
lysserthrasnleuvallysasnlyscysvalasnpheasnpheasn
530535540
glyleuthrglythrglyvalleuthrgluserasnlyslyspheleu
545550555560
propheglnglnpheglyargaspilealaaspthrthraspalaval
565570575
argaspproglnthrleugluileleuaspilethrprocysserphe
580585590
glyglyvalservalilethrproglythrasnthrserasnglnval
595600605
alavalleutyrglnaspvalasncysthrgluvalprovalalaile
610615620
hisalaaspglnleuthrprothrtrpargvaltyrserthrglyser
625630635640
asnvalpheglnthrargalaglycysleuileglyalagluhisval
645650655
asnasnsertyrglucysaspileproileglyalaglyilecysala
660665670
sertyrglnthrglnthrasnserproargargalaargservalala
675680685
serglnserileilealatyrthrmetserleuglyalagluasnser
690695700
valalatyrserasnasnserilealaileprothrasnphethrile
705710715720
servalthrthrgluileleuprovalsermetthrlysthrserval
725730735
aspcysthrmettyrilecysglyaspserthrglucysserasnleu
740745750
leuleuglntyrglyserphecysthrglnleuasnargalaleuthr
755760765
glyilealavalgluglnasplysasnthrglngluvalphealagln
770775780
vallysglnproaspproserlysproserlysargserpheileglu
785790795800
aspleuleupheasnlysvalthrleualaaspalaglypheilelys
805810815
glntyrglyaspcysleuglyaspilealaalaargaspleuilecys
820825830
alaglnlyspheasnglyleuthrvalleuproproleuleuthrasp
835840845
glumetilealaglntyrthrseralaleuleualaglythrilethr
850855860
serglytrpthrpheglyalaglyalaalaleuglnilepropheala
865870875880
metglnmetalatyrargpheasnglyileglyvalthrglnasnval
885890895
leutyrgluasnglnlysleuilealaasnglnpheasnseralaile
900905910
glylysileglnaspserleuserserthralaseralaleuglylys
915920925
leuglnaspvalvalasnglnasnalaglnalaleuasnthrleuval
930935940
lysglnleuserserasnpheglyalaileserservalleuasnasp
945950955960
ileleuserargleuaspproproglualagluvalglnileasparg
965970975
leuilethrglyargleuglnserleuglnthrtyrvalthrglngln
980985990
leuileargalaalagluileargalaseralaasnleualaalathr
99510001005
lysmetserglucysvalleuglyglnserlysargvalaspphe
101010151020
cysglylysglytyrhisleumetserpheproglnseralapro
102510301035
hisglyvalvalpheleuhisvalthrtyrvalproalaglnglu
104010451050
lysasnphethrthralaproalailecyshisaspglylysala
105510601065
hispheproarggluglyvalphevalserasnglythrhistrp
107010751080
phevalthrglnargasnphetyrgluproglnileilethrthr
108510901095
aspasnthrphevalserglyasncysaspvalvalileglyile
110011051110
valasnasnthrvaltyraspproleuglnprogluleuaspser
111511201125
phelysglugluleuasplystyrphelysasnhisthrserpro
113011351140
aspvalaspleuglyaspileserglyileasnalaservalval
114511501155
asnileglnlysgluileaspargleuasngluvalalalysasn
116011651170
leuasngluserleuileaspleuglngluleuglylystyrglu
117511801185
glntyrilelystrppro
1190
<210>9
<211>1237
<212>prt
<213>人工序列
<220>
<223>重组sars-cov-2s蛋白
<400>9
metphevalpheleuvalleuleuproleuvalserserglncysval
151015
asnleuthrthrargthrglnleuproproalatyrthrasnserphe
202530
thrargglyvaltyrtyrproasplysvalpheargserservalleu
354045
hisserthrglnaspleupheleuprophepheserasnvalthrtrp
505560
phehisalailehisvalserglythrasnglythrlysargpheasp
65707580
asnprovalleupropheasnaspglyvaltyrphealaserthrglu
859095
lysserasnileileargglytrpilepheglythrthrleuaspser
100105110
lysthrglnserleuleuilevalasnasnalathrasnvalvalile
115120125
lysvalcysglupheglnphecysasnasppropheleuglyvaltyr
130135140
tyrhislysasnasnlyssertrpmetgluserglupheargvaltyr
145150155160
serseralaasnasncysthrpheglutyrvalserglnpropheleu
165170175
metaspleugluglylysglnglyasnphelysasnleuarggluphe
180185190
valphelysasnileaspglytyrphelysiletyrserlyshisthr
195200205
proileasnleuvalargaspleuproglnglypheseralaleuglu
210215220
proleuvalaspleuproileglyileasnilethrargpheglnthr
225230235240
leuleualaleuhisargsertyrleuthrproglyaspserserser
245250255
glytrpthralaglyalaalaalatyrtyrvalglytyrleuglnpro
260265270
argthrpheleuleulystyrasngluasnglythrilethraspala
275280285
valaspcysalaleuaspproleusergluthrlyscysthrleulys
290295300
serphethrvalglulysglyiletyrglnthrserasnpheargval
305310315320
glnprothrgluserilevalargpheproasnilethrasnleucys
325330335
propheglygluvalpheasnalathrargphealaservaltyrala
340345350
trpasnarglysargileserasncysvalalaasptyrservalleu
355360365
tyrasnseralaserpheserthrphelyscystyrglyvalserpro
370375380
thrlysleuasnaspleucysphethrasnvaltyralaaspserphe
385390395400
valileargglyaspgluvalargglnilealaproglyglnthrgly
405410415
lysilealaasptyrasntyrlysleuproaspaspphethrglycys
420425430
valilealatrpasnserasnasnleuaspserlysvalglyglyasn
435440445
tyrasntyrleutyrargleuphearglysserasnleulysprophe
450455460
gluargaspileserthrgluiletyrglnalaglyserthrprocys
465470475480
asnglyvalgluglypheasncystyrpheproleuglnsertyrgly
485490495
pheglnprothrasnglyvalglytyrglnprotyrargvalvalval
500505510
leuserphegluleuleuhisalaproalathrvalcysglyprolys
515520525
lysserthrasnleuvallysasnlyscysvalasnpheasnpheasn
530535540
glyleuthrglythrglyvalleuthrgluserasnlyslyspheleu
545550555560
propheglnglnpheglyargaspilealaaspthrthraspalaval
565570575
argaspproglnthrleugluileleuaspilethrprocysserphe
580585590
glyglyvalservalilethrproglythrasnthrserasnglnval
595600605
alavalleutyrglnaspvalasncysthrgluvalprovalalaile
610615620
hisalaaspglnleuthrprothrtrpargvaltyrserthrglyser
625630635640
asnvalpheglnthrargalaglycysleuileglyalagluhisval
645650655
asnasnsertyrglucysaspileproileglyalaglyilecysala
660665670
sertyrglnthrglnthrasnserproargargalaargservalala
675680685
serglnserileilealatyrthrmetserleuglyalagluasnser
690695700
valalatyrserasnasnserilealaileprothrasnphethrile
705710715720
servalthrthrgluileleuprovalsermetthrlysthrserval
725730735
aspcysthrmettyrilecysglyaspserthrglucysserasnleu
740745750
leuleuglntyrglyserphecysthrglnleuasnargalaleuthr
755760765
glyilealavalgluglnasplysasnthrglngluvalphealagln
770775780
vallysglniletyrlysthrproproilelysasppheglyglyphe
785790795800
asnpheserglnileleuproaspproserlysproserlysargser
805810815
pheilegluaspleuleupheasnlysvalthrleualaaspalagly
820825830
pheilelysglntyrglyaspcysleuglyaspilealaalaargasp
835840845
leuilecysalaglnlyspheasnglyleuthrvalleuproproleu
850855860
leuthraspglumetilealaglntyrthrseralaleuleualagly
865870875880
thrilethrserglytrpthrpheglyalaglyalaalaleuglnile
885890895
prophealametglnmetalatyrargpheasnglyileglyvalthr
900905910
glnasnvalleutyrgluasnglnlysleuilealaasnglnpheasn
915920925
seralaileglylysileglnaspserleuserserthralaserala
930935940
leuglylysleuglnaspvalvalasnglnasnalaglnalaleuasn
945950955960
thrleuvallysglnleuserserasnpheglyalaileserserval
965970975
leuasnaspileleuserargleuasplysproglualagluvalgln
980985990
ileaspargleuilethrglyargleuglnserleuglnthrtyrval
99510001005
thrglnglnleuileargalaalagluileargalaseralaasn
101010151020
leualaalathrlysmetserglucysvalleuglyglnserlys
102510301035
argvalaspphecysglylysglytyrhisleumetserphepro
104010451050
glnseralaprohisglyvalvalpheleuhisvalthrtyrval
105510601065
proalaglnglulysasnphethrthralaproalailecyshis
107010751080
aspglylysalahispheproarggluglyvalphevalserasn
108510901095
glythrhistrpphevalthrglnargasnphetyrgluprogln
110011051110
ileilethrthraspasnthrphevalserglyasncysaspval
111511201125
valileglyilevalasnasnthrvaltyraspproleuglnpro
113011351140
gluleuaspserphelysglugluleuasplystyrphelysasn
114511501155
histhrserproaspvalaspleuglyaspileserglyileasn
116011651170
alaservalvalasnileglnlysgluileaspargleuasnglu
117511801185
valalalysasnleuasngluserleuileaspleuglngluleu
119011951200
glylystyrgluglntyrilelystrpprotrptyriletrpleu
120512101215
glypheilealaglyleuilealailevalmetvalthrilemet
122012251230
leucyscysmet
1235
<210>10
<211>1237
<212>prt
<213>人工序列
<220>
<223>重组sars-cov-2s蛋白
<400>10
metphevalpheleuvalleuleuproleuvalserserglncysval
151015
asnleuthrthrargthrglnleuproproalatyrthrasnserphe
202530
thrargglyvaltyrtyrproasplysvalpheargserservalleu
354045
hisserthrglnaspleupheleuprophepheserasnvalthrtrp
505560
phehisalailehisvalserglythrasnglythrlysargpheasp
65707580
asnprovalleupropheasnaspglyvaltyrphealaserthrglu
859095
lysserasnileileargglytrpilepheglythrthrleuaspser
100105110
lysthrglnserleuleuilevalasnasnalathrasnvalvalile
115120125
lysvalcysglupheglnphecysasnasppropheleuglyvaltyr
130135140
tyrhislysasnasnlyssertrpmetgluserglupheargvaltyr
145150155160
serseralaasnasncysthrpheglutyrvalserglnpropheleu
165170175
metaspleugluglylysglnglyasnphelysasnleuarggluphe
180185190
valphelysasnileaspglytyrphelysiletyrserlyshisthr
195200205
proileasnleuvalargaspleuproglnglypheseralaleuglu
210215220
proleuvalaspleuproileglyileasnilethrargpheglnthr
225230235240
leuleualaleuhisargsertyrleuthrproglyaspserserser
245250255
glytrpthralaglyalaalaalatyrtyrvalglytyrleuglnpro
260265270
argthrpheleuleulystyrasngluasnglythrilethraspala
275280285
valaspcysalaleuaspproleusergluthrlyscysthrleulys
290295300
serphethrvalglulysglyiletyrglnthrserasnpheargval
305310315320
glnprothrgluserilevalargpheproasnilethrasnleucys
325330335
propheglygluvalpheasnalathrargphealaservaltyrala
340345350
trpasnarglysargileserasncysvalalaasptyrservalleu
355360365
tyrasnseralaserpheserthrphelyscystyrglyvalserpro
370375380
thrlysleuasnaspleucysphethrasnvaltyralaaspserphe
385390395400
valileargglyaspgluvalargglnilealaproglyglnthrgly
405410415
lysilealaasptyrasntyrlysleuproaspaspphethrglycys
420425430
valilealatrpasnserasnasnleuaspserlysvalglyglyasn
435440445
tyrasntyrleutyrargleuphearglysserasnleulysprophe
450455460
gluargaspileserthrgluiletyrglnalaglyserthrprocys
465470475480
asnglyvalgluglypheasncystyrpheproleuglnsertyrgly
485490495
pheglnprothrasnglyvalglytyrglnprotyrargvalvalval
500505510
leuserphegluleuleuhisalaproalathrvalcysglyprolys
515520525
lysserthrasnleuvallysasnlyscysvalasnpheasnpheasn
530535540
glyleuthrglythrglyvalleuthrgluserasnlyslyspheleu
545550555560
propheglnglnpheglyargaspilealaaspthrthraspalaval
565570575
argaspproglnthrleugluileleuaspilethrprocysserphe
580585590
glyglyvalservalilethrproglythrasnthrserasnglnval
595600605
alavalleutyrglnaspvalasncysthrgluvalprovalalaile
610615620
hisalaaspglnleuthrprothrtrpargvaltyrserthrglyser
625630635640
asnvalpheglnthrargalaglycysleuileglyalagluhisval
645650655
asnasnsertyrglucysaspileproileglyalaglyilecysala
660665670
sertyrglnthrglnthrasnserproargargalaargservalala
675680685
serglnserileilealatyrthrmetserleuglyalagluasnser
690695700
valalatyrserasnasnserilealaileprothrasnphethrile
705710715720
servalthrthrgluileleuprovalsermetthrlysthrserval
725730735
aspcysthrmettyrilecysglyaspserthrglucysserasnleu
740745750
leuleuglntyrglyserphecysthrglnleuasnargalaleuthr
755760765
glyilealavalgluglnasplysasnthrglngluvalphealagln
770775780
vallysglniletyrlysthrproproilelysasppheglyglyphe
785790795800
asnpheserglnileleuproaspproserlysproserlysargser
805810815
pheilegluaspleuleupheasnlysvalthrleualaaspalagly
820825830
pheilelysglntyrglyaspcysleuglyaspilealaalaargasp
835840845
leuilecysalaglnlyspheasnglyleuthrvalleuproproleu
850855860
leuthraspglumetilealaglntyrthrseralaleuleualagly
865870875880
thrilethrserglytrpthrpheglyalaglyalaalaleuglnile
885890895
prophealametglnmetalatyrargpheasnglyileglyvalthr
900905910
glnasnvalleutyrgluasnglnlysleuilealaasnglnpheasn
915920925
seralaileglylysileglnaspserleuserserthralaserala
930935940
leuglylysleuglnaspvalvalasnglnasnalaglnalaleuasn
945950955960
thrleuvallysglnleuserserasnpheglyalaileserserval
965970975
leuasnaspileleuserargleuaspprovalglualagluvalgln
980985990
ileaspargleuilethrglyargleuglnserleuglnthrtyrval
99510001005
thrglnglnleuileargalaalagluileargalaseralaasn
101010151020
leualaalathrlysmetserglucysvalleuglyglnserlys
102510301035
argvalaspphecysglylysglytyrhisleumetserphepro
104010451050
glnseralaprohisglyvalvalpheleuhisvalthrtyrval
105510601065
proalaglnglulysasnphethrthralaproalailecyshis
107010751080
aspglylysalahispheproarggluglyvalphevalserasn
108510901095
glythrhistrpphevalthrglnargasnphetyrgluprogln
110011051110
ileilethrthraspasnthrphevalserglyasncysaspval
111511201125
valileglyilevalasnasnthrvaltyraspproleuglnpro
113011351140
gluleuaspserphelysglugluleuasplystyrphelysasn
114511501155
histhrserproaspvalaspleuglyaspileserglyileasn
116011651170
alaservalvalasnileglnlysgluileaspargleuasnglu
117511801185
valalalysasnleuasngluserleuileaspleuglngluleu
119011951200
glylystyrgluglntyrilelystrpprotrptyriletrpleu
120512101215
glypheilealaglyleuilealailevalmetvalthrilemet
122012251230
leucyscysmet
1235
<210>11
<211>1237
<212>prt
<213>人工序列
<220>
<223>重组sars-cov-2s蛋白
<400>11
metphevalpheleuvalleuleuproleuvalserserglncysval
151015
asnleuthrthrargthrglnleuproproalatyrthrasnserphe
202530
thrargglyvaltyrtyrproasplysvalpheargserservalleu
354045
hisserthrglnaspleupheleuprophepheserasnvalthrtrp
505560
phehisalailehisvalserglythrasnglythrlysargpheasp
65707580
asnprovalleupropheasnaspglyvaltyrphealaserthrglu
859095
lysserasnileileargglytrpilepheglythrthrleuaspser
100105110
lysthrglnserleuleuilevalasnasnalathrasnvalvalile
115120125
lysvalcysglupheglnphecysasnasppropheleuglyvaltyr
130135140
tyrhislysasnasnlyssertrpmetgluserglupheargvaltyr
145150155160
serseralaasnasncysthrpheglutyrvalserglnpropheleu
165170175
metaspleugluglylysglnglyasnphelysasnleuarggluphe
180185190
valphelysasnileaspglytyrphelysiletyrserlyshisthr
195200205
proileasnleuvalargaspleuproglnglypheseralaleuglu
210215220
proleuvalaspleuproileglyileasnilethrargpheglnthr
225230235240
leuleualaleuhisargsertyrleuthrproglyaspserserser
245250255
glytrpthralaglyalaalaalatyrtyrvalglytyrleuglnpro
260265270
argthrpheleuleulystyrasngluasnglythrilethraspala
275280285
valaspcysalaleuaspproleusergluthrlyscysthrleulys
290295300
serphethrvalglulysglyiletyrglnthrserasnpheargval
305310315320
glnprothrgluserilevalargpheproasnilethrasnleucys
325330335
propheglygluvalpheasnalathrargphealaservaltyrala
340345350
trpasnarglysargileserasncysvalalaasptyrservalleu
355360365
tyrasnseralaserpheserthrphelyscystyrglyvalserpro
370375380
thrlysleuasnaspleucysphethrasnvaltyralaaspserphe
385390395400
valileargglyaspgluvalargglnilealaproglyglnthrgly
405410415
lysilealaasptyrasntyrlysleuproaspaspphethrglycys
420425430
valilealatrpasnserasnasnleuaspserlysvalglyglyasn
435440445
tyrasntyrleutyrargleuphearglysserasnleulysprophe
450455460
gluargaspileserthrgluiletyrglnalaglyserthrprocys
465470475480
asnglyvalgluglypheasncystyrpheproleuglnsertyrgly
485490495
pheglnprothrasnglyvalglytyrglnprotyrargvalvalval
500505510
leuserphegluleuleuhisalaproalathrvalcysglyprolys
515520525
lysserthrasnleuvallysasnlyscysvalasnpheasnpheasn
530535540
glyleuthrglythrglyvalleuthrgluserasnlyslyspheleu
545550555560
propheglnglnpheglyargaspilealaaspthrthraspalaval
565570575
argaspproglnthrleugluileleuaspilethrprocysserphe
580585590
glyglyvalservalilethrproglythrasnthrserasnglnval
595600605
alavalleutyrglnaspvalasncysthrgluvalprovalalaile
610615620
hisalaaspglnleuthrprothrtrpargvaltyrserthrglyser
625630635640
asnvalpheglnthrargalaglycysleuileglyalagluhisval
645650655
asnasnsertyrglucysaspileproileglyalaglyilecysala
660665670
sertyrglnthrglnthrasnserproargargalaargservalala
675680685
serglnserileilealatyrthrmetserleuglyalagluasnser
690695700
valalatyrserasnasnserilealaileprothrasnphethrile
705710715720
servalthrthrgluileleuprovalsermetthrlysthrserval
725730735
aspcysthrmettyrilecysglyaspserthrglucysserasnleu
740745750
leuleuglntyrglyserphecysthrglnleuasnargalaleuthr
755760765
glyilealavalgluglnasplysasnthrglngluvalphealagln
770775780
vallysglniletyrlysthrproproilelysasppheglyglyphe
785790795800
asnpheserglnileleuproaspproserlysproserlysargser
805810815
pheilegluaspleuleupheasnlysvalthrleualaaspalagly
820825830
pheilelysglntyrglyaspcysleuglyaspilealaalaargasp
835840845
leuilecysalaglnlyspheasnglyleuthrvalleuproproleu
850855860
leuthraspglumetilealaglntyrthrseralaleuleualagly
865870875880
thrilethrserglytrpthrpheglyalaglyalaalaleuglnile
885890895
prophealametglnmetalatyrargpheasnglyileglyvalthr
900905910
glnasnvalleutyrgluasnglnlysleuilealaasnglnpheasn
915920925
seralaileglylysileglnaspserleuserserthralaserala
930935940
leuglylysleuglnaspvalvalasnglnasnalaglnalaleuasn
945950955960
thrleuvallysglnleuserserasnpheglyalaileserserval
965970975
leuasnaspileleuserargleuaspproproglualagluvalgln
980985990
ileaspargleuilethrglyargleuglnserleuglnthrtyrval
99510001005
thrglnglnleuileargalaalagluileargalaseralaasn
101010151020
leualaalathrlysmetserglucysvalleuglyglnserlys
102510301035
argvalaspphecysglylysglytyrhisleumetserphepro
104010451050
glnseralaprohisglyvalvalpheleuhisvalthrtyrval
105510601065
proalaglnglulysasnphethrthralaproalailecyshis
107010751080
aspglylysalahispheproarggluglyvalphevalserasn
108510901095
glythrhistrpphevalthrglnargasnphetyrgluprogln
110011051110
ileilethrthraspasnthrphevalserglyasncysaspval
111511201125
valileglyilevalasnasnthrvaltyraspproleuglnpro
113011351140
gluleuaspserphelysglugluleuasplystyrphelysasn
114511501155
histhrserproaspvalaspleuglyaspileserglyileasn
116011651170
alaservalvalasnileglnlysgluileaspargleuasnglu
117511801185
valalalysasnleuasngluserleuileaspleuglngluleu
119011951200
glylystyrgluglntyrilelystrpprotrptyriletrpleu
120512101215
glypheilealaglyleuilealailevalmetvalthrilemet
122012251230
leucyscysmet
1235
<210>12
<211>1218
<212>prt
<213>人工序列
<220>
<223>重组sars-cov-2s蛋白
<400>12
metphevalpheleuvalleuleuproleuvalserserglncysval
151015
asnleuthrthrargthrglnleuproproalatyrthrasnserphe
202530
thrargglyvaltyrtyrproasplysvalpheargserservalleu
354045
hisserthrglnaspleupheleuprophepheserasnvalthrtrp
505560
phehisalailehisvalserglythrasnglythrlysargpheasp
65707580
asnprovalleupropheasnaspglyvaltyrphealaserthrglu
859095
lysserasnileileargglytrpilepheglythrthrleuaspser
100105110
lysthrglnserleuleuilevalasnasnalathrasnvalvalile
115120125
lysvalcysglupheglnphecysasnasppropheleuglyvaltyr
130135140
tyrhislysasnasnlyssertrpmetgluserglupheargvaltyr
145150155160
serseralaasnasncysthrpheglutyrvalserglnpropheleu
165170175
metaspleugluglylysglnglyasnphelysasnleuarggluphe
180185190
valphelysasnileaspglytyrphelysiletyrserlyshisthr
195200205
proileasnleuvalargaspleuproglnglypheseralaleuglu
210215220
proleuvalaspleuproileglyileasnilethrargpheglnthr
225230235240
leuleualaleuhisargsertyrleuthrproglyaspserserser
245250255
glytrpthralaglyalaalaalatyrtyrvalglytyrleuglnpro
260265270
argthrpheleuleulystyrasngluasnglythrilethraspala
275280285
valaspcysalaleuaspproleusergluthrlyscysthrleulys
290295300
serphethrvalglulysglyiletyrglnthrserasnpheargval
305310315320
glnprothrgluserilevalargpheproasnilethrasnleucys
325330335
propheglygluvalpheasnalathrargphealaservaltyrala
340345350
trpasnarglysargileserasncysvalalaasptyrservalleu
355360365
tyrasnseralaserpheserthrphelyscystyrglyvalserpro
370375380
thrlysleuasnaspleucysphethrasnvaltyralaaspserphe
385390395400
valileargglyaspgluvalargglnilealaproglyglnthrgly
405410415
lysilealaasptyrasntyrlysleuproaspaspphethrglycys
420425430
valilealatrpasnserasnasnleuaspserlysvalglyglyasn
435440445
tyrasntyrleutyrargleuphearglysserasnleulysprophe
450455460
gluargaspileserthrgluiletyrglnalaglyserthrprocys
465470475480
asnglyvalgluglypheasncystyrpheproleuglnsertyrgly
485490495
pheglnprothrasnglyvalglytyrglnprotyrargvalvalval
500505510
leuserphegluleuleuhisalaproalathrvalcysglyprolys
515520525
lysserthrasnleuvallysasnlyscysvalasnpheasnpheasn
530535540
glyleuthrglythrglyvalleuthrgluserasnlyslyspheleu
545550555560
propheglnglnpheglyargaspilealaaspthrthraspalaval
565570575
argaspproglnthrleugluileleuaspilethrprocysserphe
580585590
glyglyvalservalilethrproglythrasnthrserasnglnval
595600605
alavalleutyrglnaspvalasncysthrgluvalprovalalaile
610615620
hisalaaspglnleuthrprothrtrpargvaltyrserthrglyser
625630635640
asnvalpheglnthrargalaglycysleuileglyalagluhisval
645650655
asnasnsertyrglucysaspileproileglyalaglyilecysala
660665670
sertyrglnthrglnthrasnserproargargalaargservalala
675680685
serglnserileilealatyrthrmetserleuglyalagluasnser
690695700
valalatyrserasnasnserilealaileprothrasnphethrile
705710715720
servalthrthrgluileleuprovalsermetthrlysthrserval
725730735
aspcysthrmettyrilecysglyaspserthrglucysserasnleu
740745750
leuleuglntyrglyserphecysthrglnleuasnargalaleuthr
755760765
glyilealavalgluglnasplysasnthrglngluvalphealagln
770775780
vallysglnproaspproserlysproserlysargserpheileglu
785790795800
aspleuleupheasnlysvalthrleualaaspalaglypheilelys
805810815
glntyrglyaspcysleuglyaspilealaalaargaspleuilecys
820825830
alaglnlyspheasnglyleuthrvalleuproproleuleuthrasp
835840845
glumetilealaglntyrthrseralaleuleualaglythrilethr
850855860
serglytrpthrpheglyalaglyalaalaleuglnilepropheala
865870875880
metglnmetalatyrargpheasnglyileglyvalthrglnasnval
885890895
leutyrgluasnglnlysleuilealaasnglnpheasnseralaile
900905910
glylysileglnaspserleuserserthralaseralaleuglylys
915920925
leuglnaspvalvalasnglnasnalaglnalaleuasnthrleuval
930935940
lysglnleuserserasnpheglyalaileserservalleuasnasp
945950955960
ileleuserargleuaspprovalglualagluvalglnileasparg
965970975
leuilethrglyargleuglnserleuglnthrtyrvalthrglngln
980985990
leuileargalaalagluileargalaseralaasnleualaalathr
99510001005
lysmetserglucysvalleuglyglnserlysargvalaspphe
101010151020
cysglylysglytyrhisleumetserpheproglnseralapro
102510301035
hisglyvalvalpheleuhisvalthrtyrvalproalaglnglu
104010451050
lysasnphethrthralaproalailecyshisaspglylysala
105510601065
hispheproarggluglyvalphevalserasnglythrhistrp
107010751080
phevalthrglnargasnphetyrgluproglnileilethrthr
108510901095
aspasnthrphevalserglyasncysaspvalvalileglyile
110011051110
valasnasnthrvaltyraspproleuglnprogluleuaspser
111511201125
phelysglugluleuasplystyrphelysasnhisthrserpro
113011351140
aspvalaspleuglyaspileserglyileasnalaservalval
114511501155
asnileglnlysgluileaspargleuasngluvalalalysasn
116011651170
leuasngluserleuileaspleuglngluleuglylystyrglu
117511801185
glntyrilelystrpprotrptyriletrpleuglypheileala
119011951200
glyleuilealailevalmetvalthrilemetleucyscysmet
120512101215
<210>13
<211>1218
<212>prt
<213>人工序列
<220>
<223>重组sars-cov-2s蛋白
<400>13
metphevalpheleuvalleuleuproleuvalserserglncysval
151015
asnleuthrthrargthrglnleuproproalatyrthrasnserphe
202530
thrargglyvaltyrtyrproasplysvalpheargserservalleu
354045
hisserthrglnaspleupheleuprophepheserasnvalthrtrp
505560
phehisalailehisvalserglythrasnglythrlysargpheasp
65707580
asnprovalleupropheasnaspglyvaltyrphealaserthrglu
859095
lysserasnileileargglytrpilepheglythrthrleuaspser
100105110
lysthrglnserleuleuilevalasnasnalathrasnvalvalile
115120125
lysvalcysglupheglnphecysasnasppropheleuglyvaltyr
130135140
tyrhislysasnasnlyssertrpmetgluserglupheargvaltyr
145150155160
serseralaasnasncysthrpheglutyrvalserglnpropheleu
165170175
metaspleugluglylysglnglyasnphelysasnleuarggluphe
180185190
valphelysasnileaspglytyrphelysiletyrserlyshisthr
195200205
proileasnleuvalargaspleuproglnglypheseralaleuglu
210215220
proleuvalaspleuproileglyileasnilethrargpheglnthr
225230235240
leuleualaleuhisargsertyrleuthrproglyaspserserser
245250255
glytrpthralaglyalaalaalatyrtyrvalglytyrleuglnpro
260265270
argthrpheleuleulystyrasngluasnglythrilethraspala
275280285
valaspcysalaleuaspproleusergluthrlyscysthrleulys
290295300
serphethrvalglulysglyiletyrglnthrserasnpheargval
305310315320
glnprothrgluserilevalargpheproasnilethrasnleucys
325330335
propheglygluvalpheasnalathrargphealaservaltyrala
340345350
trpasnarglysargileserasncysvalalaasptyrservalleu
355360365
tyrasnseralaserpheserthrphelyscystyrglyvalserpro
370375380
thrlysleuasnaspleucysphethrasnvaltyralaaspserphe
385390395400
valileargglyaspgluvalargglnilealaproglyglnthrgly
405410415
lysilealaasptyrasntyrlysleuproaspaspphethrglycys
420425430
valilealatrpasnserasnasnleuaspserlysvalglyglyasn
435440445
tyrasntyrleutyrargleuphearglysserasnleulysprophe
450455460
gluargaspileserthrgluiletyrglnalaglyserthrprocys
465470475480
asnglyvalgluglypheasncystyrpheproleuglnsertyrgly
485490495
pheglnprothrasnglyvalglytyrglnprotyrargvalvalval
500505510
leuserphegluleuleuhisalaproalathrvalcysglyprolys
515520525
lysserthrasnleuvallysasnlyscysvalasnpheasnpheasn
530535540
glyleuthrglythrglyvalleuthrgluserasnlyslyspheleu
545550555560
propheglnglnpheglyargaspilealaaspthrthraspalaval
565570575
argaspproglnthrleugluileleuaspilethrprocysserphe
580585590
glyglyvalservalilethrproglythrasnthrserasnglnval
595600605
alavalleutyrglnaspvalasncysthrgluvalprovalalaile
610615620
hisalaaspglnleuthrprothrtrpargvaltyrserthrglyser
625630635640
asnvalpheglnthrargalaglycysleuileglyalagluhisval
645650655
asnasnsertyrglucysaspileproileglyalaglyilecysala
660665670
sertyrglnthrglnthrasnserproargargalaargservalala
675680685
serglnserileilealatyrthrmetserleuglyalagluasnser
690695700
valalatyrserasnasnserilealaileprothrasnphethrile
705710715720
servalthrthrgluileleuprovalsermetthrlysthrserval
725730735
aspcysthrmettyrilecysglyaspserthrglucysserasnleu
740745750
leuleuglntyrglyserphecysthrglnleuasnargalaleuthr
755760765
glyilealavalgluglnasplysasnthrglngluvalphealagln
770775780
vallysglnproaspproserlysproserlysargserpheileglu
785790795800
aspleuleupheasnlysvalthrleualaaspalaglypheilelys
805810815
glntyrglyaspcysleuglyaspilealaalaargaspleuilecys
820825830
alaglnlyspheasnglyleuthrvalleuproproleuleuthrasp
835840845
glumetilealaglntyrthrseralaleuleualaglythrilethr
850855860
serglytrpthrpheglyalaglyalaalaleuglnilepropheala
865870875880
metglnmetalatyrargpheasnglyileglyvalthrglnasnval
885890895
leutyrgluasnglnlysleuilealaasnglnpheasnseralaile
900905910
glylysileglnaspserleuserserthralaseralaleuglylys
915920925
leuglnaspvalvalasnglnasnalaglnalaleuasnthrleuval
930935940
lysglnleuserserasnpheglyalaileserservalleuasnasp
945950955960
ileleuserargleuasplysproglualagluvalglnileasparg
965970975
leuilethrglyargleuglnserleuglnthrtyrvalthrglngln
980985990
leuileargalaalagluileargalaseralaasnleualaalathr
99510001005
lysmetserglucysvalleuglyglnserlysargvalaspphe
101010151020
cysglylysglytyrhisleumetserpheproglnseralapro
102510301035
hisglyvalvalpheleuhisvalthrtyrvalproalaglnglu
104010451050
lysasnphethrthralaproalailecyshisaspglylysala
105510601065
hispheproarggluglyvalphevalserasnglythrhistrp
107010751080
phevalthrglnargasnphetyrgluproglnileilethrthr
108510901095
aspasnthrphevalserglyasncysaspvalvalileglyile
110011051110
valasnasnthrvaltyraspproleuglnprogluleuaspser
111511201125
phelysglugluleuasplystyrphelysasnhisthrserpro
113011351140
aspvalaspleuglyaspileserglyileasnalaservalval
114511501155
asnileglnlysgluileaspargleuasngluvalalalysasn
116011651170
leuasngluserleuileaspleuglngluleuglylystyrglu
117511801185
glntyrilelystrpprotrptyriletrpleuglypheileala
119011951200
glyleuilealailevalmetvalthrilemetleucyscysmet
120512101215
<210>14
<211>1218
<212>prt
<213>人工序列
<220>
<223>重组sars-cov-2s蛋白
<400>14
metphevalpheleuvalleuleuproleuvalserserglncysval
151015
asnleuthrthrargthrglnleuproproalatyrthrasnserphe
202530
thrargglyvaltyrtyrproasplysvalpheargserservalleu
354045
hisserthrglnaspleupheleuprophepheserasnvalthrtrp
505560
phehisalailehisvalserglythrasnglythrlysargpheasp
65707580
asnprovalleupropheasnaspglyvaltyrphealaserthrglu
859095
lysserasnileileargglytrpilepheglythrthrleuaspser
100105110
lysthrglnserleuleuilevalasnasnalathrasnvalvalile
115120125
lysvalcysglupheglnphecysasnasppropheleuglyvaltyr
130135140
tyrhislysasnasnlyssertrpmetgluserglupheargvaltyr
145150155160
serseralaasnasncysthrpheglutyrvalserglnpropheleu
165170175
metaspleugluglylysglnglyasnphelysasnleuarggluphe
180185190
valphelysasnileaspglytyrphelysiletyrserlyshisthr
195200205
proileasnleuvalargaspleuproglnglypheseralaleuglu
210215220
proleuvalaspleuproileglyileasnilethrargpheglnthr
225230235240
leuleualaleuhisargsertyrleuthrproglyaspserserser
245250255
glytrpthralaglyalaalaalatyrtyrvalglytyrleuglnpro
260265270
argthrpheleuleulystyrasngluasnglythrilethraspala
275280285
valaspcysalaleuaspproleusergluthrlyscysthrleulys
290295300
serphethrvalglulysglyiletyrglnthrserasnpheargval
305310315320
glnprothrgluserilevalargpheproasnilethrasnleucys
325330335
propheglygluvalpheasnalathrargphealaservaltyrala
340345350
trpasnarglysargileserasncysvalalaasptyrservalleu
355360365
tyrasnseralaserpheserthrphelyscystyrglyvalserpro
370375380
thrlysleuasnaspleucysphethrasnvaltyralaaspserphe
385390395400
valileargglyaspgluvalargglnilealaproglyglnthrgly
405410415
lysilealaasptyrasntyrlysleuproaspaspphethrglycys
420425430
valilealatrpasnserasnasnleuaspserlysvalglyglyasn
435440445
tyrasntyrleutyrargleuphearglysserasnleulysprophe
450455460
gluargaspileserthrgluiletyrglnalaglyserthrprocys
465470475480
asnglyvalgluglypheasncystyrpheproleuglnsertyrgly
485490495
pheglnprothrasnglyvalglytyrglnprotyrargvalvalval
500505510
leuserphegluleuleuhisalaproalathrvalcysglyprolys
515520525
lysserthrasnleuvallysasnlyscysvalasnpheasnpheasn
530535540
glyleuthrglythrglyvalleuthrgluserasnlyslyspheleu
545550555560
propheglnglnpheglyargaspilealaaspthrthraspalaval
565570575
argaspproglnthrleugluileleuaspilethrprocysserphe
580585590
glyglyvalservalilethrproglythrasnthrserasnglnval
595600605
alavalleutyrglnaspvalasncysthrgluvalprovalalaile
610615620
hisalaaspglnleuthrprothrtrpargvaltyrserthrglyser
625630635640
asnvalpheglnthrargalaglycysleuileglyalagluhisval
645650655
asnasnsertyrglucysaspileproileglyalaglyilecysala
660665670
sertyrglnthrglnthrasnserproargargalaargservalala
675680685
serglnserileilealatyrthrmetserleuglyalagluasnser
690695700
valalatyrserasnasnserilealaileprothrasnphethrile
705710715720
servalthrthrgluileleuprovalsermetthrlysthrserval
725730735
aspcysthrmettyrilecysglyaspserthrglucysserasnleu
740745750
leuleuglntyrglyserphecysthrglnleuasnargalaleuthr
755760765
glyilealavalgluglnasplysasnthrglngluvalphealagln
770775780
vallysglnproaspproserlysproserlysargserpheileglu
785790795800
aspleuleupheasnlysvalthrleualaaspalaglypheilelys
805810815
glntyrglyaspcysleuglyaspilealaalaargaspleuilecys
820825830
alaglnlyspheasnglyleuthrvalleuproproleuleuthrasp
835840845
glumetilealaglntyrthrseralaleuleualaglythrilethr
850855860
serglytrpthrpheglyalaglyalaalaleuglnilepropheala
865870875880
metglnmetalatyrargpheasnglyileglyvalthrglnasnval
885890895
leutyrgluasnglnlysleuilealaasnglnpheasnseralaile
900905910
glylysileglnaspserleuserserthralaseralaleuglylys
915920925
leuglnaspvalvalasnglnasnalaglnalaleuasnthrleuval
930935940
lysglnleuserserasnpheglyalaileserservalleuasnasp
945950955960
ileleuserargleuaspproproglualagluvalglnileasparg
965970975
leuilethrglyargleuglnserleuglnthrtyrvalthrglngln
980985990
leuileargalaalagluileargalaseralaasnleualaalathr
99510001005
lysmetserglucysvalleuglyglnserlysargvalaspphe
101010151020
cysglylysglytyrhisleumetserpheproglnseralapro
102510301035
hisglyvalvalpheleuhisvalthrtyrvalproalaglnglu
104010451050
lysasnphethrthralaproalailecyshisaspglylysala
105510601065
hispheproarggluglyvalphevalserasnglythrhistrp
107010751080
phevalthrglnargasnphetyrgluproglnileilethrthr
108510901095
aspasnthrphevalserglyasncysaspvalvalileglyile
110011051110
valasnasnthrvaltyraspproleuglnprogluleuaspser
111511201125
phelysglugluleuasplystyrphelysasnhisthrserpro
113011351140
aspvalaspleuglyaspileserglyileasnalaservalval
114511501155
asnileglnlysgluileaspargleuasngluvalalalysasn
116011651170
leuasngluserleuileaspleuglngluleuglylystyrglu
117511801185
glntyrilelystrpprotrptyriletrpleuglypheileala
119011951200
glyleuilealailevalmetvalthrilemetleucyscysmet
120512101215
<210>15
<211>1213
<212>prt
<213>人工序列
<220>
<223>重组sars-cov-2s蛋白
<400>15
metphevalpheleuvalleuleuproleuvalserserglncysval
151015
asnleuthrthrargthrglnleuproproalatyrthrasnserphe
202530
thrargglyvaltyrtyrproasplysvalpheargserservalleu
354045
hisserthrglnaspleupheleuprophepheserasnvalthrtrp
505560
phehisalailehisvalserglythrasnglythrlysargpheasp
65707580
asnprovalleupropheasnaspglyvaltyrphealaserthrglu
859095
lysserasnileileargglytrpilepheglythrthrleuaspser
100105110
lysthrglnserleuleuilevalasnasnalathrasnvalvalile
115120125
lysvalcysglupheglnphecysasnasppropheleuglyvaltyr
130135140
tyrhislysasnasnlyssertrpmetgluserglupheargvaltyr
145150155160
serseralaasnasncysthrpheglutyrvalserglnpropheleu
165170175
metaspleugluglylysglnglyasnphelysasnleuarggluphe
180185190
valphelysasnileaspglytyrphelysiletyrserlyshisthr
195200205
proileasnleuvalargaspleuproglnglypheseralaleuglu
210215220
proleuvalaspleuproileglyileasnilethrargpheglnthr
225230235240
leuleualaleuhisargsertyrleuthrproglyaspserserser
245250255
glytrpthralaglyalaalaalatyrtyrvalglytyrleuglnpro
260265270
argthrpheleuleulystyrasngluasnglythrilethraspala
275280285
valaspcysalaleuaspproleusergluthrlyscysthrleulys
290295300
serphethrvalglulysglyiletyrglnthrserasnpheargval
305310315320
glnprothrgluserilevalargpheproasnilethrasnleucys
325330335
propheglygluvalpheasnalathrargphealaservaltyrala
340345350
trpasnarglysargileserasncysvalalaasptyrservalleu
355360365
tyrasnseralaserpheserthrphelyscystyrglyvalserpro
370375380
thrlysleuasnaspleucysphethrasnvaltyralaaspserphe
385390395400
valileargglyaspgluvalargglnilealaproglyglnthrgly
405410415
lysilealaasptyrasntyrlysleuproaspaspphethrglycys
420425430
valilealatrpasnserasnasnleuaspserlysvalglyglyasn
435440445
tyrasntyrleutyrargleuphearglysserasnleulysprophe
450455460
gluargaspileserthrgluiletyrglnalaglyserthrprocys
465470475480
asnglyvalgluglypheasncystyrpheproleuglnsertyrgly
485490495
pheglnprothrasnglyvalglytyrglnprotyrargvalvalval
500505510
leuserphegluleuleuhisalaproalathrvalcysglyprolys
515520525
lysserthrasnleuvallysasnlyscysvalasnpheasnpheasn
530535540
glyleuthrglythrglyvalleuthrgluserasnlyslyspheleu
545550555560
propheglnglnpheglyargaspilealaaspthrthraspalaval
565570575
argaspproglnthrleugluileleuaspilethrprocysserphe
580585590
glyglyvalservalilethrproglythrasnthrserasnglnval
595600605
alavalleutyrglnaspvalasncysthrgluvalprovalalaile
610615620
hisalaaspglnleuthrprothrtrpargvaltyrserthrglyser
625630635640
asnvalpheglnthrargalaglycysleuileglyalagluhisval
645650655
asnasnsertyrglucysaspileproileglyalaglyilecysala
660665670
sertyrglnthrglnthrasnserproglyglyserglyservalala
675680685
serglnserileilealatyrthrmetserleuglyalagluasnser
690695700
valalatyrserasnasnserilealaileprothrasnphethrile
705710715720
servalthrthrgluileleuprovalsermetthrlysthrserval
725730735
aspcysthrmettyrilecysglyaspserthrglucysserasnleu
740745750
leuleuglntyrglyserphecysthrglnleuasnargalaleuthr
755760765
glyilealavalgluglnasplysasnthrglngluvalphealagln
770775780
vallysglniletyrlysthrproproilelysasppheglyglyphe
785790795800
asnpheserglnileleuproaspproserlysproserlysargser
805810815
pheilegluaspleuleupheasnlysvalthrleualaaspalagly
820825830
pheilelysglntyrglyaspcysleuglyaspilealaalaargasp
835840845
leuilecysalaglnlyspheasnglyleuthrvalleuproproleu
850855860
leuthraspglumetilealaglntyrthrseralaleuleualagly
865870875880
thrilethrserglytrpthrpheglyalaglyalaalaleuglnile
885890895
prophealametglnmetalatyrargpheasnglyileglyvalthr
900905910
glnasnvalleutyrgluasnglnlysleuilealaasnglnpheasn
915920925
seralaileglylysileglnaspserleuserserthralaserala
930935940
leuglylysleuglnaspvalvalasnglnasnalaglnalaleuasn
945950955960
thrleuvallysglnleuserserasnpheglyalaileserserval
965970975
leuasnaspileleuserargleuaspprovalglualagluvalgln
980985990
ileaspargleuilethrglyargleuglnserleuglnthrtyrval
99510001005
thrglnglnleuileargalaalagluileargalaseralaasn
101010151020
leualaalathrlysmetserglucysvalleuglyglnserlys
102510301035
argvalaspphecysglylysglytyrhisleumetserphepro
104010451050
glnseralaprohisglyvalvalpheleuhisvalthrtyrval
105510601065
proalaglnglulysasnphethrthralaproalailecyshis
107010751080
aspglylysalahispheproarggluglyvalphevalserasn
108510901095
glythrhistrpphevalthrglnargasnphetyrgluprogln
110011051110
ileilethrthraspasnthrphevalserglyasncysaspval
111511201125
valileglyilevalasnasnthrvaltyraspproleuglnpro
113011351140
gluleuaspserphelysglugluleuasplystyrphelysasn
114511501155
histhrserproaspvalaspleuglyaspileserglyileasn
116011651170
alaservalvalasnileglnlysgluileaspargleuasnglu
117511801185
valalalysasnleuasngluserleuileaspleuglngluleu
119011951200
glylystyrgluglntyrilelystrppro
12051210
<210>16
<211>1213
<212>prt
<213>人工序列
<220>
<223>重组sars-cov-2s蛋白
<400>16
metphevalpheleuvalleuleuproleuvalserserglncysval
151015
asnleuthrthrargthrglnleuproproalatyrthrasnserphe
202530
thrargglyvaltyrtyrproasplysvalpheargserservalleu
354045
hisserthrglnaspleupheleuprophepheserasnvalthrtrp
505560
phehisalailehisvalserglythrasnglythrlysargpheasp
65707580
asnprovalleupropheasnaspglyvaltyrphealaserthrglu
859095
lysserasnileileargglytrpilepheglythrthrleuaspser
100105110
lysthrglnserleuleuilevalasnasnalathrasnvalvalile
115120125
lysvalcysglupheglnphecysasnasppropheleuglyvaltyr
130135140
tyrhislysasnasnlyssertrpmetgluserglupheargvaltyr
145150155160
serseralaasnasncysthrpheglutyrvalserglnpropheleu
165170175
metaspleugluglylysglnglyasnphelysasnleuarggluphe
180185190
valphelysasnileaspglytyrphelysiletyrserlyshisthr
195200205
proileasnleuvalargaspleuproglnglypheseralaleuglu
210215220
proleuvalaspleuproileglyileasnilethrargpheglnthr
225230235240
leuleualaleuhisargsertyrleuthrproglyaspserserser
245250255
glytrpthralaglyalaalaalatyrtyrvalglytyrleuglnpro
260265270
argthrpheleuleulystyrasngluasnglythrilethraspala
275280285
valaspcysalaleuaspproleusergluthrlyscysthrleulys
290295300
serphethrvalglulysglyiletyrglnthrserasnpheargval
305310315320
glnprothrgluserilevalargpheproasnilethrasnleucys
325330335
propheglygluvalpheasnalathrargphealaservaltyrala
340345350
trpasnarglysargileserasncysvalalaasptyrservalleu
355360365
tyrasnseralaserpheserthrphelyscystyrglyvalserpro
370375380
thrlysleuasnaspleucysphethrasnvaltyralaaspserphe
385390395400
valileargglyaspgluvalargglnilealaproglyglnthrgly
405410415
lysilealaasptyrasntyrlysleuproaspaspphethrglycys
420425430
valilealatrpasnserasnasnleuaspserlysvalglyglyasn
435440445
tyrasntyrleutyrargleuphearglysserasnleulysprophe
450455460
gluargaspileserthrgluiletyrglnalaglyserthrprocys
465470475480
asnglyvalgluglypheasncystyrpheproleuglnsertyrgly
485490495
pheglnprothrasnglyvalglytyrglnprotyrargvalvalval
500505510
leuserphegluleuleuhisalaproalathrvalcysglyprolys
515520525
lysserthrasnleuvallysasnlyscysvalasnpheasnpheasn
530535540
glyleuthrglythrglyvalleuthrgluserasnlyslyspheleu
545550555560
propheglnglnpheglyargaspilealaaspthrthraspalaval
565570575
argaspproglnthrleugluileleuaspilethrprocysserphe
580585590
glyglyvalservalilethrproglythrasnthrserasnglnval
595600605
alavalleutyrglnaspvalasncysthrgluvalprovalalaile
610615620
hisalaaspglnleuthrprothrtrpargvaltyrserthrglyser
625630635640
asnvalpheglnthrargalaglycysleuileglyalagluhisval
645650655
asnasnsertyrglucysaspileproileglyalaglyilecysala
660665670
sertyrglnthrglnthrasnserproglyglyserglyservalala
675680685
serglnserileilealatyrthrmetserleuglyalagluasnser
690695700
valalatyrserasnasnserilealaileprothrasnphethrile
705710715720
servalthrthrgluileleuprovalsermetthrlysthrserval
725730735
aspcysthrmettyrilecysglyaspserthrglucysserasnleu
740745750
leuleuglntyrglyserphecysthrglnleuasnargalaleuthr
755760765
glyilealavalgluglnasplysasnthrglngluvalphealagln
770775780
vallysglniletyrlysthrproproilelysasppheglyglyphe
785790795800
asnpheserglnileleuproaspproserlysproserlysargser
805810815
pheilegluaspleuleupheasnlysvalthrleualaaspalagly
820825830
pheilelysglntyrglyaspcysleuglyaspilealaalaargasp
835840845
leuilecysalaglnlyspheasnglyleuthrvalleuproproleu
850855860
leuthraspglumetilealaglntyrthrseralaleuleualagly
865870875880
thrilethrserglytrpthrpheglyalaglyalaalaleuglnile
885890895
prophealametglnmetalatyrargpheasnglyileglyvalthr
900905910
glnasnvalleutyrgluasnglnlysleuilealaasnglnpheasn
915920925
seralaileglylysileglnaspserleuserserthralaserala
930935940
leuglylysleuglnaspvalvalasnglnasnalaglnalaleuasn
945950955960
thrleuvallysglnleuserserasnpheglyalaileserserval
965970975
leuasnaspileleuserargleuasplysproglualagluvalgln
980985990
ileaspargleuilethrglyargleuglnserleuglnthrtyrval
99510001005
thrglnglnleuileargalaalagluileargalaseralaasn
101010151020
leualaalathrlysmetserglucysvalleuglyglnserlys
102510301035
argvalaspphecysglylysglytyrhisleumetserphepro
104010451050
glnseralaprohisglyvalvalpheleuhisvalthrtyrval
105510601065
proalaglnglulysasnphethrthralaproalailecyshis
107010751080
aspglylysalahispheproarggluglyvalphevalserasn
108510901095
glythrhistrpphevalthrglnargasnphetyrgluprogln
110011051110
ileilethrthraspasnthrphevalserglyasncysaspval
111511201125
valileglyilevalasnasnthrvaltyraspproleuglnpro
113011351140
gluleuaspserphelysglugluleuasplystyrphelysasn
114511501155
histhrserproaspvalaspleuglyaspileserglyileasn
116011651170
alaservalvalasnileglnlysgluileaspargleuasnglu
117511801185
valalalysasnleuasngluserleuileaspleuglngluleu
119011951200
glylystyrgluglntyrilelystrppro
12051210
<210>17
<211>1213
<212>prt
<213>人工序列
<220>
<223>重组sars-cov-2s蛋白
<400>17
metphevalpheleuvalleuleuproleuvalserserglncysval
151015
asnleuthrthrargthrglnleuproproalatyrthrasnserphe
202530
thrargglyvaltyrtyrproasplysvalpheargserservalleu
354045
hisserthrglnaspleupheleuprophepheserasnvalthrtrp
505560
phehisalailehisvalserglythrasnglythrlysargpheasp
65707580
asnprovalleupropheasnaspglyvaltyrphealaserthrglu
859095
lysserasnileileargglytrpilepheglythrthrleuaspser
100105110
lysthrglnserleuleuilevalasnasnalathrasnvalvalile
115120125
lysvalcysglupheglnphecysasnasppropheleuglyvaltyr
130135140
tyrhislysasnasnlyssertrpmetgluserglupheargvaltyr
145150155160
serseralaasnasncysthrpheglutyrvalserglnpropheleu
165170175
metaspleugluglylysglnglyasnphelysasnleuarggluphe
180185190
valphelysasnileaspglytyrphelysiletyrserlyshisthr
195200205
proileasnleuvalargaspleuproglnglypheseralaleuglu
210215220
proleuvalaspleuproileglyileasnilethrargpheglnthr
225230235240
leuleualaleuhisargsertyrleuthrproglyaspserserser
245250255
glytrpthralaglyalaalaalatyrtyrvalglytyrleuglnpro
260265270
argthrpheleuleulystyrasngluasnglythrilethraspala
275280285
valaspcysalaleuaspproleusergluthrlyscysthrleulys
290295300
serphethrvalglulysglyiletyrglnthrserasnpheargval
305310315320
glnprothrgluserilevalargpheproasnilethrasnleucys
325330335
propheglygluvalpheasnalathrargphealaservaltyrala
340345350
trpasnarglysargileserasncysvalalaasptyrservalleu
355360365
tyrasnseralaserpheserthrphelyscystyrglyvalserpro
370375380
thrlysleuasnaspleucysphethrasnvaltyralaaspserphe
385390395400
valileargglyaspgluvalargglnilealaproglyglnthrgly
405410415
lysilealaasptyrasntyrlysleuproaspaspphethrglycys
420425430
valilealatrpasnserasnasnleuaspserlysvalglyglyasn
435440445
tyrasntyrleutyrargleuphearglysserasnleulysprophe
450455460
gluargaspileserthrgluiletyrglnalaglyserthrprocys
465470475480
asnglyvalgluglypheasncystyrpheproleuglnsertyrgly
485490495
pheglnprothrasnglyvalglytyrglnprotyrargvalvalval
500505510
leuserphegluleuleuhisalaproalathrvalcysglyprolys
515520525
lysserthrasnleuvallysasnlyscysvalasnpheasnpheasn
530535540
glyleuthrglythrglyvalleuthrgluserasnlyslyspheleu
545550555560
propheglnglnpheglyargaspilealaaspthrthraspalaval
565570575
argaspproglnthrleugluileleuaspilethrprocysserphe
580585590
glyglyvalservalilethrproglythrasnthrserasnglnval
595600605
alavalleutyrglnaspvalasncysthrgluvalprovalalaile
610615620
hisalaaspglnleuthrprothrtrpargvaltyrserthrglyser
625630635640
asnvalpheglnthrargalaglycysleuileglyalagluhisval
645650655
asnasnsertyrglucysaspileproileglyalaglyilecysala
660665670
sertyrglnthrglnthrasnserproglyglyserglyservalala
675680685
serglnserileilealatyrthrmetserleuglyalagluasnser
690695700
valalatyrserasnasnserilealaileprothrasnphethrile
705710715720
servalthrthrgluileleuprovalsermetthrlysthrserval
725730735
aspcysthrmettyrilecysglyaspserthrglucysserasnleu
740745750
leuleuglntyrglyserphecysthrglnleuasnargalaleuthr
755760765
glyilealavalgluglnasplysasnthrglngluvalphealagln
770775780
vallysglniletyrlysthrproproilelysasppheglyglyphe
785790795800
asnpheserglnileleuproaspproserlysproserlysargser
805810815
pheilegluaspleuleupheasnlysvalthrleualaaspalagly
820825830
pheilelysglntyrglyaspcysleuglyaspilealaalaargasp
835840845
leuilecysalaglnlyspheasnglyleuthrvalleuproproleu
850855860
leuthraspglumetilealaglntyrthrseralaleuleualagly
865870875880
thrilethrserglytrpthrpheglyalaglyalaalaleuglnile
885890895
prophealametglnmetalatyrargpheasnglyileglyvalthr
900905910
glnasnvalleutyrgluasnglnlysleuilealaasnglnpheasn
915920925
seralaileglylysileglnaspserleuserserthralaserala
930935940
leuglylysleuglnaspvalvalasnglnasnalaglnalaleuasn
945950955960
thrleuvallysglnleuserserasnpheglyalaileserserval
965970975
leuasnaspileleuserargleuaspproproglualagluvalgln
980985990
ileaspargleuilethrglyargleuglnserleuglnthrtyrval
99510001005
thrglnglnleuileargalaalagluileargalaseralaasn
101010151020
leualaalathrlysmetserglucysvalleuglyglnserlys
102510301035
argvalaspphecysglylysglytyrhisleumetserphepro
104010451050
glnseralaprohisglyvalvalpheleuhisvalthrtyrval
105510601065
proalaglnglulysasnphethrthralaproalailecyshis
107010751080
aspglylysalahispheproarggluglyvalphevalserasn
108510901095
glythrhistrpphevalthrglnargasnphetyrgluprogln
110011051110
ileilethrthraspasnthrphevalserglyasncysaspval
111511201125
valileglyilevalasnasnthrvaltyraspproleuglnpro
113011351140
gluleuaspserphelysglugluleuasplystyrphelysasn
114511501155
histhrserproaspvalaspleuglyaspileserglyileasn
116011651170
alaservalvalasnileglnlysgluileaspargleuasnglu
117511801185
valalalysasnleuasngluserleuileaspleuglngluleu
119011951200
glylystyrgluglntyrilelystrppro
12051210
<210>18
<211>1194
<212>prt
<213>人工序列
<220>
<223>重组sars-cov-2s蛋白
<400>18
metphevalpheleuvalleuleuproleuvalserserglncysval
151015
asnleuthrthrargthrglnleuproproalatyrthrasnserphe
202530
thrargglyvaltyrtyrproasplysvalpheargserservalleu
354045
hisserthrglnaspleupheleuprophepheserasnvalthrtrp
505560
phehisalailehisvalserglythrasnglythrlysargpheasp
65707580
asnprovalleupropheasnaspglyvaltyrphealaserthrglu
859095
lysserasnileileargglytrpilepheglythrthrleuaspser
100105110
lysthrglnserleuleuilevalasnasnalathrasnvalvalile
115120125
lysvalcysglupheglnphecysasnasppropheleuglyvaltyr
130135140
tyrhislysasnasnlyssertrpmetgluserglupheargvaltyr
145150155160
serseralaasnasncysthrpheglutyrvalserglnpropheleu
165170175
metaspleugluglylysglnglyasnphelysasnleuarggluphe
180185190
valphelysasnileaspglytyrphelysiletyrserlyshisthr
195200205
proileasnleuvalargaspleuproglnglypheseralaleuglu
210215220
proleuvalaspleuproileglyileasnilethrargpheglnthr
225230235240
leuleualaleuhisargsertyrleuthrproglyaspserserser
245250255
glytrpthralaglyalaalaalatyrtyrvalglytyrleuglnpro
260265270
argthrpheleuleulystyrasngluasnglythrilethraspala
275280285
valaspcysalaleuaspproleusergluthrlyscysthrleulys
290295300
serphethrvalglulysglyiletyrglnthrserasnpheargval
305310315320
glnprothrgluserilevalargpheproasnilethrasnleucys
325330335
propheglygluvalpheasnalathrargphealaservaltyrala
340345350
trpasnarglysargileserasncysvalalaasptyrservalleu
355360365
tyrasnseralaserpheserthrphelyscystyrglyvalserpro
370375380
thrlysleuasnaspleucysphethrasnvaltyralaaspserphe
385390395400
valileargglyaspgluvalargglnilealaproglyglnthrgly
405410415
lysilealaasptyrasntyrlysleuproaspaspphethrglycys
420425430
valilealatrpasnserasnasnleuaspserlysvalglyglyasn
435440445
tyrasntyrleutyrargleuphearglysserasnleulysprophe
450455460
gluargaspileserthrgluiletyrglnalaglyserthrprocys
465470475480
asnglyvalgluglypheasncystyrpheproleuglnsertyrgly
485490495
pheglnprothrasnglyvalglytyrglnprotyrargvalvalval
500505510
leuserphegluleuleuhisalaproalathrvalcysglyprolys
515520525
lysserthrasnleuvallysasnlyscysvalasnpheasnpheasn
530535540
glyleuthrglythrglyvalleuthrgluserasnlyslyspheleu
545550555560
propheglnglnpheglyargaspilealaaspthrthraspalaval
565570575
argaspproglnthrleugluileleuaspilethrprocysserphe
580585590
glyglyvalservalilethrproglythrasnthrserasnglnval
595600605
alavalleutyrglnaspvalasncysthrgluvalprovalalaile
610615620
hisalaaspglnleuthrprothrtrpargvaltyrserthrglyser
625630635640
asnvalpheglnthrargalaglycysleuileglyalagluhisval
645650655
asnasnsertyrglucysaspileproileglyalaglyilecysala
660665670
sertyrglnthrglnthrasnserproglyglyserglyservalala
675680685
serglnserileilealatyrthrmetserleuglyalagluasnser
690695700
valalatyrserasnasnserilealaileprothrasnphethrile
705710715720
servalthrthrgluileleuprovalsermetthrlysthrserval
725730735
aspcysthrmettyrilecysglyaspserthrglucysserasnleu
740745750
leuleuglntyrglyserphecysthrglnleuasnargalaleuthr
755760765
glyilealavalgluglnasplysasnthrglngluvalphealagln
770775780
vallysglnproaspproserlysproserlysargserpheileglu
785790795800
aspleuleupheasnlysvalthrleualaaspalaglypheilelys
805810815
glntyrglyaspcysleuglyaspilealaalaargaspleuilecys
820825830
alaglnlyspheasnglyleuthrvalleuproproleuleuthrasp
835840845
glumetilealaglntyrthrseralaleuleualaglythrilethr
850855860
serglytrpthrpheglyalaglyalaalaleuglnilepropheala
865870875880
metglnmetalatyrargpheasnglyileglyvalthrglnasnval
885890895
leutyrgluasnglnlysleuilealaasnglnpheasnseralaile
900905910
glylysileglnaspserleuserserthralaseralaleuglylys
915920925
leuglnaspvalvalasnglnasnalaglnalaleuasnthrleuval
930935940
lysglnleuserserasnpheglyalaileserservalleuasnasp
945950955960
ileleuserargleuaspprovalglualagluvalglnileasparg
965970975
leuilethrglyargleuglnserleuglnthrtyrvalthrglngln
980985990
leuileargalaalagluileargalaseralaasnleualaalathr
99510001005
lysmetserglucysvalleuglyglnserlysargvalaspphe
101010151020
cysglylysglytyrhisleumetserpheproglnseralapro
102510301035
hisglyvalvalpheleuhisvalthrtyrvalproalaglnglu
104010451050
lysasnphethrthralaproalailecyshisaspglylysala
105510601065
hispheproarggluglyvalphevalserasnglythrhistrp
107010751080
phevalthrglnargasnphetyrgluproglnileilethrthr
108510901095
aspasnthrphevalserglyasncysaspvalvalileglyile
110011051110
valasnasnthrvaltyraspproleuglnprogluleuaspser
111511201125
phelysglugluleuasplystyrphelysasnhisthrserpro
113011351140
aspvalaspleuglyaspileserglyileasnalaservalval
114511501155
asnileglnlysgluileaspargleuasngluvalalalysasn
116011651170
leuasngluserleuileaspleuglngluleuglylystyrglu
117511801185
glntyrilelystrppro
1190
<210>19
<211>1194
<212>prt
<213>人工序列
<220>
<223>重组sars-cov-2s蛋白
<400>19
metphevalpheleuvalleuleuproleuvalserserglncysval
151015
asnleuthrthrargthrglnleuproproalatyrthrasnserphe
202530
thrargglyvaltyrtyrproasplysvalpheargserservalleu
354045
hisserthrglnaspleupheleuprophepheserasnvalthrtrp
505560
phehisalailehisvalserglythrasnglythrlysargpheasp
65707580
asnprovalleupropheasnaspglyvaltyrphealaserthrglu
859095
lysserasnileileargglytrpilepheglythrthrleuaspser
100105110
lysthrglnserleuleuilevalasnasnalathrasnvalvalile
115120125
lysvalcysglupheglnphecysasnasppropheleuglyvaltyr
130135140
tyrhislysasnasnlyssertrpmetgluserglupheargvaltyr
145150155160
serseralaasnasncysthrpheglutyrvalserglnpropheleu
165170175
metaspleugluglylysglnglyasnphelysasnleuarggluphe
180185190
valphelysasnileaspglytyrphelysiletyrserlyshisthr
195200205
proileasnleuvalargaspleuproglnglypheseralaleuglu
210215220
proleuvalaspleuproileglyileasnilethrargpheglnthr
225230235240
leuleualaleuhisargsertyrleuthrproglyaspserserser
245250255
glytrpthralaglyalaalaalatyrtyrvalglytyrleuglnpro
260265270
argthrpheleuleulystyrasngluasnglythrilethraspala
275280285
valaspcysalaleuaspproleusergluthrlyscysthrleulys
290295300
serphethrvalglulysglyiletyrglnthrserasnpheargval
305310315320
glnprothrgluserilevalargpheproasnilethrasnleucys
325330335
propheglygluvalpheasnalathrargphealaservaltyrala
340345350
trpasnarglysargileserasncysvalalaasptyrservalleu
355360365
tyrasnseralaserpheserthrphelyscystyrglyvalserpro
370375380
thrlysleuasnaspleucysphethrasnvaltyralaaspserphe
385390395400
valileargglyaspgluvalargglnilealaproglyglnthrgly
405410415
lysilealaasptyrasntyrlysleuproaspaspphethrglycys
420425430
valilealatrpasnserasnasnleuaspserlysvalglyglyasn
435440445
tyrasntyrleutyrargleuphearglysserasnleulysprophe
450455460
gluargaspileserthrgluiletyrglnalaglyserthrprocys
465470475480
asnglyvalgluglypheasncystyrpheproleuglnsertyrgly
485490495
pheglnprothrasnglyvalglytyrglnprotyrargvalvalval
500505510
leuserphegluleuleuhisalaproalathrvalcysglyprolys
515520525
lysserthrasnleuvallysasnlyscysvalasnpheasnpheasn
530535540
glyleuthrglythrglyvalleuthrgluserasnlyslyspheleu
545550555560
propheglnglnpheglyargaspilealaaspthrthraspalaval
565570575
argaspproglnthrleugluileleuaspilethrprocysserphe
580585590
glyglyvalservalilethrproglythrasnthrserasnglnval
595600605
alavalleutyrglnaspvalasncysthrgluvalprovalalaile
610615620
hisalaaspglnleuthrprothrtrpargvaltyrserthrglyser
625630635640
asnvalpheglnthrargalaglycysleuileglyalagluhisval
645650655
asnasnsertyrglucysaspileproileglyalaglyilecysala
660665670
sertyrglnthrglnthrasnserproglyglyserglyservalala
675680685
serglnserileilealatyrthrmetserleuglyalagluasnser
690695700
valalatyrserasnasnserilealaileprothrasnphethrile
705710715720
servalthrthrgluileleuprovalsermetthrlysthrserval
725730735
aspcysthrmettyrilecysglyaspserthrglucysserasnleu
740745750
leuleuglntyrglyserphecysthrglnleuasnargalaleuthr
755760765
glyilealavalgluglnasplysasnthrglngluvalphealagln
770775780
vallysglnproaspproserlysproserlysargserpheileglu
785790795800
aspleuleupheasnlysvalthrleualaaspalaglypheilelys
805810815
glntyrglyaspcysleuglyaspilealaalaargaspleuilecys
820825830
alaglnlyspheasnglyleuthrvalleuproproleuleuthrasp
835840845
glumetilealaglntyrthrseralaleuleualaglythrilethr
850855860
serglytrpthrpheglyalaglyalaalaleuglnilepropheala
865870875880
metglnmetalatyrargpheasnglyileglyvalthrglnasnval
885890895
leutyrgluasnglnlysleuilealaasnglnpheasnseralaile
900905910
glylysileglnaspserleuserserthralaseralaleuglylys
915920925
leuglnaspvalvalasnglnasnalaglnalaleuasnthrleuval
930935940
lysglnleuserserasnpheglyalaileserservalleuasnasp
945950955960
ileleuserargleuasplysproglualagluvalglnileasparg
965970975
leuilethrglyargleuglnserleuglnthrtyrvalthrglngln
980985990
leuileargalaalagluileargalaseralaasnleualaalathr
99510001005
lysmetserglucysvalleuglyglnserlysargvalaspphe
101010151020
cysglylysglytyrhisleumetserpheproglnseralapro
102510301035
hisglyvalvalpheleuhisvalthrtyrvalproalaglnglu
104010451050
lysasnphethrthralaproalailecyshisaspglylysala
105510601065
hispheproarggluglyvalphevalserasnglythrhistrp
107010751080
phevalthrglnargasnphetyrgluproglnileilethrthr
108510901095
aspasnthrphevalserglyasncysaspvalvalileglyile
110011051110
valasnasnthrvaltyraspproleuglnprogluleuaspser
111511201125
phelysglugluleuasplystyrphelysasnhisthrserpro
113011351140
aspvalaspleuglyaspileserglyileasnalaservalval
114511501155
asnileglnlysgluileaspargleuasngluvalalalysasn
116011651170
leuasngluserleuileaspleuglngluleuglylystyrglu
117511801185
glntyrilelystrppro
1190
<210>20
<211>1194
<212>prt
<213>人工序列
<220>
<223>重组sars-cov-2s蛋白
<400>20
metphevalpheleuvalleuleuproleuvalserserglncysval
151015
asnleuthrthrargthrglnleuproproalatyrthrasnserphe
202530
thrargglyvaltyrtyrproasplysvalpheargserservalleu
354045
hisserthrglnaspleupheleuprophepheserasnvalthrtrp
505560
phehisalailehisvalserglythrasnglythrlysargpheasp
65707580
asnprovalleupropheasnaspglyvaltyrphealaserthrglu
859095
lysserasnileileargglytrpilepheglythrthrleuaspser
100105110
lysthrglnserleuleuilevalasnasnalathrasnvalvalile
115120125
lysvalcysglupheglnphecysasnasppropheleuglyvaltyr
130135140
tyrhislysasnasnlyssertrpmetgluserglupheargvaltyr
145150155160
serseralaasnasncysthrpheglutyrvalserglnpropheleu
165170175
metaspleugluglylysglnglyasnphelysasnleuarggluphe
180185190
valphelysasnileaspglytyrphelysiletyrserlyshisthr
195200205
proileasnleuvalargaspleuproglnglypheseralaleuglu
210215220
proleuvalaspleuproileglyileasnilethrargpheglnthr
225230235240
leuleualaleuhisargsertyrleuthrproglyaspserserser
245250255
glytrpthralaglyalaalaalatyrtyrvalglytyrleuglnpro
260265270
argthrpheleuleulystyrasngluasnglythrilethraspala
275280285
valaspcysalaleuaspproleusergluthrlyscysthrleulys
290295300
serphethrvalglulysglyiletyrglnthrserasnpheargval
305310315320
glnprothrgluserilevalargpheproasnilethrasnleucys
325330335
propheglygluvalpheasnalathrargphealaservaltyrala
340345350
trpasnarglysargileserasncysvalalaasptyrservalleu
355360365
tyrasnseralaserpheserthrphelyscystyrglyvalserpro
370375380
thrlysleuasnaspleucysphethrasnvaltyralaaspserphe
385390395400
valileargglyaspgluvalargglnilealaproglyglnthrgly
405410415
lysilealaasptyrasntyrlysleuproaspaspphethrglycys
420425430
valilealatrpasnserasnasnleuaspserlysvalglyglyasn
435440445
tyrasntyrleutyrargleuphearglysserasnleulysprophe
450455460
gluargaspileserthrgluiletyrglnalaglyserthrprocys
465470475480
asnglyvalgluglypheasncystyrpheproleuglnsertyrgly
485490495
pheglnprothrasnglyvalglytyrglnprotyrargvalvalval
500505510
leuserphegluleuleuhisalaproalathrvalcysglyprolys
515520525
lysserthrasnleuvallysasnlyscysvalasnpheasnpheasn
530535540
glyleuthrglythrglyvalleuthrgluserasnlyslyspheleu
545550555560
propheglnglnpheglyargaspilealaaspthrthraspalaval
565570575
argaspproglnthrleugluileleuaspilethrprocysserphe
580585590
glyglyvalservalilethrproglythrasnthrserasnglnval
595600605
alavalleutyrglnaspvalasncysthrgluvalprovalalaile
610615620
hisalaaspglnleuthrprothrtrpargvaltyrserthrglyser
625630635640
asnvalpheglnthrargalaglycysleuileglyalagluhisval
645650655
asnasnsertyrglucysaspileproileglyalaglyilecysala
660665670
sertyrglnthrglnthrasnserproglyglyserglyservalala
675680685
serglnserileilealatyrthrmetserleuglyalagluasnser
690695700
valalatyrserasnasnserilealaileprothrasnphethrile
705710715720
servalthrthrgluileleuprovalsermetthrlysthrserval
725730735
aspcysthrmettyrilecysglyaspserthrglucysserasnleu
740745750
leuleuglntyrglyserphecysthrglnleuasnargalaleuthr
755760765
glyilealavalgluglnasplysasnthrglngluvalphealagln
770775780
vallysglnproaspproserlysproserlysargserpheileglu
785790795800
aspleuleupheasnlysvalthrleualaaspalaglypheilelys
805810815
glntyrglyaspcysleuglyaspilealaalaargaspleuilecys
820825830
alaglnlyspheasnglyleuthrvalleuproproleuleuthrasp
835840845
glumetilealaglntyrthrseralaleuleualaglythrilethr
850855860
serglytrpthrpheglyalaglyalaalaleuglnilepropheala
865870875880
metglnmetalatyrargpheasnglyileglyvalthrglnasnval
885890895
leutyrgluasnglnlysleuilealaasnglnpheasnseralaile
900905910
glylysileglnaspserleuserserthralaseralaleuglylys
915920925
leuglnaspvalvalasnglnasnalaglnalaleuasnthrleuval
930935940
lysglnleuserserasnpheglyalaileserservalleuasnasp
945950955960
ileleuserargleuaspproproglualagluvalglnileasparg
965970975
leuilethrglyargleuglnserleuglnthrtyrvalthrglngln
980985990
leuileargalaalagluileargalaseralaasnleualaalathr
99510001005
lysmetserglucysvalleuglyglnserlysargvalaspphe
101010151020
cysglylysglytyrhisleumetserpheproglnseralapro
102510301035
hisglyvalvalpheleuhisvalthrtyrvalproalaglnglu
104010451050
lysasnphethrthralaproalailecyshisaspglylysala
105510601065
hispheproarggluglyvalphevalserasnglythrhistrp
107010751080
phevalthrglnargasnphetyrgluproglnileilethrthr
108510901095
aspasnthrphevalserglyasncysaspvalvalileglyile
110011051110
valasnasnthrvaltyraspproleuglnprogluleuaspser
111511201125
phelysglugluleuasplystyrphelysasnhisthrserpro
113011351140
aspvalaspleuglyaspileserglyileasnalaservalval
114511501155
asnileglnlysgluileaspargleuasngluvalalalysasn
116011651170
leuasngluserleuileaspleuglngluleuglylystyrglu
117511801185
glntyrilelystrppro
1190
<210>21
<211>1237
<212>prt
<213>人工序列
<220>
<223>重组sars-cov-2s蛋白
<400>21
metphevalpheleuvalleuleuproleuvalserserglncysval
151015
asnleuthrthrargthrglnleuproproalatyrthrasnserphe
202530
thrargglyvaltyrtyrproasplysvalpheargserservalleu
354045
hisserthrglnaspleupheleuprophepheserasnvalthrtrp
505560
phehisalailehisvalserglythrasnglythrlysargpheasp
65707580
asnprovalleupropheasnaspglyvaltyrphealaserthrglu
859095
lysserasnileileargglytrpilepheglythrthrleuaspser
100105110
lysthrglnserleuleuilevalasnasnalathrasnvalvalile
115120125
lysvalcysglupheglnphecysasnasppropheleuglyvaltyr
130135140
tyrhislysasnasnlyssertrpmetgluserglupheargvaltyr
145150155160
serseralaasnasncysthrpheglutyrvalserglnpropheleu
165170175
metaspleugluglylysglnglyasnphelysasnleuarggluphe
180185190
valphelysasnileaspglytyrphelysiletyrserlyshisthr
195200205
proileasnleuvalargaspleuproglnglypheseralaleuglu
210215220
proleuvalaspleuproileglyileasnilethrargpheglnthr
225230235240
leuleualaleuhisargsertyrleuthrproglyaspserserser
245250255
glytrpthralaglyalaalaalatyrtyrvalglytyrleuglnpro
260265270
argthrpheleuleulystyrasngluasnglythrilethraspala
275280285
valaspcysalaleuaspproleusergluthrlyscysthrleulys
290295300
serphethrvalglulysglyiletyrglnthrserasnpheargval
305310315320
glnprothrgluserilevalargpheproasnilethrasnleucys
325330335
propheglygluvalpheasnalathrargphealaservaltyrala
340345350
trpasnarglysargileserasncysvalalaasptyrservalleu
355360365
tyrasnseralaserpheserthrphelyscystyrglyvalserpro
370375380
thrlysleuasnaspleucysphethrasnvaltyralaaspserphe
385390395400
valileargglyaspgluvalargglnilealaproglyglnthrgly
405410415
lysilealaasptyrasntyrlysleuproaspaspphethrglycys
420425430
valilealatrpasnserasnasnleuaspserlysvalglyglyasn
435440445
tyrasntyrleutyrargleuphearglysserasnleulysprophe
450455460
gluargaspileserthrgluiletyrglnalaglyserthrprocys
465470475480
asnglyvalgluglypheasncystyrpheproleuglnsertyrgly
485490495
pheglnprothrasnglyvalglytyrglnprotyrargvalvalval
500505510
leuserphegluleuleuhisalaproalathrvalcysglyprolys
515520525
lysserthrasnleuvallysasnlyscysvalasnpheasnpheasn
530535540
glyleuthrglythrglyvalleuthrgluserasnlyslyspheleu
545550555560
propheglnglnpheglyargaspilealaaspthrthraspalaval
565570575
argaspproglnthrleugluileleuaspilethrprocysserphe
580585590
glyglyvalservalilethrproglythrasnthrserasnglnval
595600605
alavalleutyrglnaspvalasncysthrgluvalprovalalaile
610615620
hisalaaspglnleuthrprothrtrpargvaltyrserthrglyser
625630635640
asnvalpheglnthrargalaglycysleuileglyalagluhisval
645650655
asnasnsertyrglucysaspileproileglyalaglyilecysala
660665670
sertyrglnthrglnthrasnserproglyglyserglyservalala
675680685
serglnserileilealatyrthrmetserleuglyalagluasnser
690695700
valalatyrserasnasnserilealaileprothrasnphethrile
705710715720
servalthrthrgluileleuprovalsermetthrlysthrserval
725730735
aspcysthrmettyrilecysglyaspserthrglucysserasnleu
740745750
leuleuglntyrglyserphecysthrglnleuasnargalaleuthr
755760765
glyilealavalgluglnasplysasnthrglngluvalphealagln
770775780
vallysglniletyrlysthrproproilelysasppheglyglyphe
785790795800
asnpheserglnileleuproaspproserlysproserlysargser
805810815
pheilegluaspleuleupheasnlysvalthrleualaaspalagly
820825830
pheilelysglntyrglyaspcysleuglyaspilealaalaargasp
835840845
leuilecysalaglnlyspheasnglyleuthrvalleuproproleu
850855860
leuthraspglumetilealaglntyrthrseralaleuleualagly
865870875880
thrilethrserglytrpthrpheglyalaglyalaalaleuglnile
885890895
prophealametglnmetalatyrargpheasnglyileglyvalthr
900905910
glnasnvalleutyrgluasnglnlysleuilealaasnglnpheasn
915920925
seralaileglylysileglnaspserleuserserthralaserala
930935940
leuglylysleuglnaspvalvalasnglnasnalaglnalaleuasn
945950955960
thrleuvallysglnleuserserasnpheglyalaileserserval
965970975
leuasnaspileleuserargleuasplysproglualagluvalgln
980985990
ileaspargleuilethrglyargleuglnserleuglnthrtyrval
99510001005
thrglnglnleuileargalaalagluileargalaseralaasn
101010151020
leualaalathrlysmetserglucysvalleuglyglnserlys
102510301035
argvalaspphecysglylysglytyrhisleumetserphepro
104010451050
glnseralaprohisglyvalvalpheleuhisvalthrtyrval
105510601065
proalaglnglulysasnphethrthralaproalailecyshis
107010751080
aspglylysalahispheproarggluglyvalphevalserasn
108510901095
glythrhistrpphevalthrglnargasnphetyrgluprogln
110011051110
ileilethrthraspasnthrphevalserglyasncysaspval
111511201125
valileglyilevalasnasnthrvaltyraspproleuglnpro
113011351140
gluleuaspserphelysglugluleuasplystyrphelysasn
114511501155
histhrserproaspvalaspleuglyaspileserglyileasn
116011651170
alaservalvalasnileglnlysgluileaspargleuasnglu
117511801185
valalalysasnleuasngluserleuileaspleuglngluleu
119011951200
glylystyrgluglntyrilelystrpprotrptyriletrpleu
120512101215
glypheilealaglyleuilealailevalmetvalthrilemet
122012251230
leucyscysmet
1235
<210>22
<211>1237
<212>prt
<213>人工序列
<220>
<223>重组sars-cov-2s蛋白
<400>22
metphevalpheleuvalleuleuproleuvalserserglncysval
151015
asnleuthrthrargthrglnleuproproalatyrthrasnserphe
202530
thrargglyvaltyrtyrproasplysvalpheargserservalleu
354045
hisserthrglnaspleupheleuprophepheserasnvalthrtrp
505560
phehisalailehisvalserglythrasnglythrlysargpheasp
65707580
asnprovalleupropheasnaspglyvaltyrphealaserthrglu
859095
lysserasnileileargglytrpilepheglythrthrleuaspser
100105110
lysthrglnserleuleuilevalasnasnalathrasnvalvalile
115120125
lysvalcysglupheglnphecysasnasppropheleuglyvaltyr
130135140
tyrhislysasnasnlyssertrpmetgluserglupheargvaltyr
145150155160
serseralaasnasncysthrpheglutyrvalserglnpropheleu
165170175
metaspleugluglylysglnglyasnphelysasnleuarggluphe
180185190
valphelysasnileaspglytyrphelysiletyrserlyshisthr
195200205
proileasnleuvalargaspleuproglnglypheseralaleuglu
210215220
proleuvalaspleuproileglyileasnilethrargpheglnthr
225230235240
leuleualaleuhisargsertyrleuthrproglyaspserserser
245250255
glytrpthralaglyalaalaalatyrtyrvalglytyrleuglnpro
260265270
argthrpheleuleulystyrasngluasnglythrilethraspala
275280285
valaspcysalaleuaspproleusergluthrlyscysthrleulys
290295300
serphethrvalglulysglyiletyrglnthrserasnpheargval
305310315320
glnprothrgluserilevalargpheproasnilethrasnleucys
325330335
propheglygluvalpheasnalathrargphealaservaltyrala
340345350
trpasnarglysargileserasncysvalalaasptyrservalleu
355360365
tyrasnseralaserpheserthrphelyscystyrglyvalserpro
370375380
thrlysleuasnaspleucysphethrasnvaltyralaaspserphe
385390395400
valileargglyaspgluvalargglnilealaproglyglnthrgly
405410415
lysilealaasptyrasntyrlysleuproaspaspphethrglycys
420425430
valilealatrpasnserasnasnleuaspserlysvalglyglyasn
435440445
tyrasntyrleutyrargleuphearglysserasnleulysprophe
450455460
gluargaspileserthrgluiletyrglnalaglyserthrprocys
465470475480
asnglyvalgluglypheasncystyrpheproleuglnsertyrgly
485490495
pheglnprothrasnglyvalglytyrglnprotyrargvalvalval
500505510
leuserphegluleuleuhisalaproalathrvalcysglyprolys
515520525
lysserthrasnleuvallysasnlyscysvalasnpheasnpheasn
530535540
glyleuthrglythrglyvalleuthrgluserasnlyslyspheleu
545550555560
propheglnglnpheglyargaspilealaaspthrthraspalaval
565570575
argaspproglnthrleugluileleuaspilethrprocysserphe
580585590
glyglyvalservalilethrproglythrasnthrserasnglnval
595600605
alavalleutyrglnaspvalasncysthrgluvalprovalalaile
610615620
hisalaaspglnleuthrprothrtrpargvaltyrserthrglyser
625630635640
asnvalpheglnthrargalaglycysleuileglyalagluhisval
645650655
asnasnsertyrglucysaspileproileglyalaglyilecysala
660665670
sertyrglnthrglnthrasnserproglyglyserglyservalala
675680685
serglnserileilealatyrthrmetserleuglyalagluasnser
690695700
valalatyrserasnasnserilealaileprothrasnphethrile
705710715720
servalthrthrgluileleuprovalsermetthrlysthrserval
725730735
aspcysthrmettyrilecysglyaspserthrglucysserasnleu
740745750
leuleuglntyrglyserphecysthrglnleuasnargalaleuthr
755760765
glyilealavalgluglnasplysasnthrglngluvalphealagln
770775780
vallysglniletyrlysthrproproilelysasppheglyglyphe
785790795800
asnpheserglnileleuproaspproserlysproserlysargser
805810815
pheilegluaspleuleupheasnlysvalthrleualaaspalagly
820825830
pheilelysglntyrglyaspcysleuglyaspilealaalaargasp
835840845
leuilecysalaglnlyspheasnglyleuthrvalleuproproleu
850855860
leuthraspglumetilealaglntyrthrseralaleuleualagly
865870875880
thrilethrserglytrpthrpheglyalaglyalaalaleuglnile
885890895
prophealametglnmetalatyrargpheasnglyileglyvalthr
900905910
glnasnvalleutyrgluasnglnlysleuilealaasnglnpheasn
915920925
seralaileglylysileglnaspserleuserserthralaserala
930935940
leuglylysleuglnaspvalvalasnglnasnalaglnalaleuasn
945950955960
thrleuvallysglnleuserserasnpheglyalaileserserval
965970975
leuasnaspileleuserargleuaspprovalglualagluvalgln
980985990
ileaspargleuilethrglyargleuglnserleuglnthrtyrval
99510001005
thrglnglnleuileargalaalagluileargalaseralaasn
101010151020
leualaalathrlysmetserglucysvalleuglyglnserlys
102510301035
argvalaspphecysglylysglytyrhisleumetserphepro
104010451050
glnseralaprohisglyvalvalpheleuhisvalthrtyrval
105510601065
proalaglnglulysasnphethrthralaproalailecyshis
107010751080
aspglylysalahispheproarggluglyvalphevalserasn
108510901095
glythrhistrpphevalthrglnargasnphetyrgluprogln
110011051110
ileilethrthraspasnthrphevalserglyasncysaspval
111511201125
valileglyilevalasnasnthrvaltyraspproleuglnpro
113011351140
gluleuaspserphelysglugluleuasplystyrphelysasn
114511501155
histhrserproaspvalaspleuglyaspileserglyileasn
116011651170
alaservalvalasnileglnlysgluileaspargleuasnglu
117511801185
valalalysasnleuasngluserleuileaspleuglngluleu
119011951200
glylystyrgluglntyrilelystrpprotrptyriletrpleu
120512101215
glypheilealaglyleuilealailevalmetvalthrilemet
122012251230
leucyscysmet
1235
<210>23
<211>1237
<212>prt
<213>人工序列
<220>
<223>重组sars-cov-2s蛋白
<400>23
metphevalpheleuvalleuleuproleuvalserserglncysval
151015
asnleuthrthrargthrglnleuproproalatyrthrasnserphe
202530
thrargglyvaltyrtyrproasplysvalpheargserservalleu
354045
hisserthrglnaspleupheleuprophepheserasnvalthrtrp
505560
phehisalailehisvalserglythrasnglythrlysargpheasp
65707580
asnprovalleupropheasnaspglyvaltyrphealaserthrglu
859095
lysserasnileileargglytrpilepheglythrthrleuaspser
100105110
lysthrglnserleuleuilevalasnasnalathrasnvalvalile
115120125
lysvalcysglupheglnphecysasnasppropheleuglyvaltyr
130135140
tyrhislysasnasnlyssertrpmetgluserglupheargvaltyr
145150155160
serseralaasnasncysthrpheglutyrvalserglnpropheleu
165170175
metaspleugluglylysglnglyasnphelysasnleuarggluphe
180185190
valphelysasnileaspglytyrphelysiletyrserlyshisthr
195200205
proileasnleuvalargaspleuproglnglypheseralaleuglu
210215220
proleuvalaspleuproileglyileasnilethrargpheglnthr
225230235240
leuleualaleuhisargsertyrleuthrproglyaspserserser
245250255
glytrpthralaglyalaalaalatyrtyrvalglytyrleuglnpro
260265270
argthrpheleuleulystyrasngluasnglythrilethraspala
275280285
valaspcysalaleuaspproleusergluthrlyscysthrleulys
290295300
serphethrvalglulysglyiletyrglnthrserasnpheargval
305310315320
glnprothrgluserilevalargpheproasnilethrasnleucys
325330335
propheglygluvalpheasnalathrargphealaservaltyrala
340345350
trpasnarglysargileserasncysvalalaasptyrservalleu
355360365
tyrasnseralaserpheserthrphelyscystyrglyvalserpro
370375380
thrlysleuasnaspleucysphethrasnvaltyralaaspserphe
385390395400
valileargglyaspgluvalargglnilealaproglyglnthrgly
405410415
lysilealaasptyrasntyrlysleuproaspaspphethrglycys
420425430
valilealatrpasnserasnasnleuaspserlysvalglyglyasn
435440445
tyrasntyrleutyrargleuphearglysserasnleulysprophe
450455460
gluargaspileserthrgluiletyrglnalaglyserthrprocys
465470475480
asnglyvalgluglypheasncystyrpheproleuglnsertyrgly
485490495
pheglnprothrasnglyvalglytyrglnprotyrargvalvalval
500505510
leuserphegluleuleuhisalaproalathrvalcysglyprolys
515520525
lysserthrasnleuvallysasnlyscysvalasnpheasnpheasn
530535540
glyleuthrglythrglyvalleuthrgluserasnlyslyspheleu
545550555560
propheglnglnpheglyargaspilealaaspthrthraspalaval
565570575
argaspproglnthrleugluileleuaspilethrprocysserphe
580585590
glyglyvalservalilethrproglythrasnthrserasnglnval
595600605
alavalleutyrglnaspvalasncysthrgluvalprovalalaile
610615620
hisalaaspglnleuthrprothrtrpargvaltyrserthrglyser
625630635640
asnvalpheglnthrargalaglycysleuileglyalagluhisval
645650655
asnasnsertyrglucysaspileproileglyalaglyilecysala
660665670
sertyrglnthrglnthrasnserproglyglyserglyservalala
675680685
serglnserileilealatyrthrmetserleuglyalagluasnser
690695700
valalatyrserasnasnserilealaileprothrasnphethrile
705710715720
servalthrthrgluileleuprovalsermetthrlysthrserval
725730735
aspcysthrmettyrilecysglyaspserthrglucysserasnleu
740745750
leuleuglntyrglyserphecysthrglnleuasnargalaleuthr
755760765
glyilealavalgluglnasplysasnthrglngluvalphealagln
770775780
vallysglniletyrlysthrproproilelysasppheglyglyphe
785790795800
asnpheserglnileleuproaspproserlysproserlysargser
805810815
pheilegluaspleuleupheasnlysvalthrleualaaspalagly
820825830
pheilelysglntyrglyaspcysleuglyaspilealaalaargasp
835840845
leuilecysalaglnlyspheasnglyleuthrvalleuproproleu
850855860
leuthraspglumetilealaglntyrthrseralaleuleualagly
865870875880
thrilethrserglytrpthrpheglyalaglyalaalaleuglnile
885890895
prophealametglnmetalatyrargpheasnglyileglyvalthr
900905910
glnasnvalleutyrgluasnglnlysleuilealaasnglnpheasn
915920925
seralaileglylysileglnaspserleuserserthralaserala
930935940
leuglylysleuglnaspvalvalasnglnasnalaglnalaleuasn
945950955960
thrleuvallysglnleuserserasnpheglyalaileserserval
965970975
leuasnaspileleuserargleuaspproproglualagluvalgln
980985990
ileaspargleuilethrglyargleuglnserleuglnthrtyrval
99510001005
thrglnglnleuileargalaalagluileargalaseralaasn
101010151020
leualaalathrlysmetserglucysvalleuglyglnserlys
102510301035
argvalaspphecysglylysglytyrhisleumetserphepro
104010451050
glnseralaprohisglyvalvalpheleuhisvalthrtyrval
105510601065
proalaglnglulysasnphethrthralaproalailecyshis
107010751080
aspglylysalahispheproarggluglyvalphevalserasn
108510901095
glythrhistrpphevalthrglnargasnphetyrgluprogln
110011051110
ileilethrthraspasnthrphevalserglyasncysaspval
111511201125
valileglyilevalasnasnthrvaltyraspproleuglnpro
113011351140
gluleuaspserphelysglugluleuasplystyrphelysasn
114511501155
histhrserproaspvalaspleuglyaspileserglyileasn
116011651170
alaservalvalasnileglnlysgluileaspargleuasnglu
117511801185
valalalysasnleuasngluserleuileaspleuglngluleu
119011951200
glylystyrgluglntyrilelystrpprotrptyriletrpleu
120512101215
glypheilealaglyleuilealailevalmetvalthrilemet
122012251230
leucyscysmet
1235
<210>24
<211>1218
<212>prt
<213>人工序列
<220>
<223>重组sars-cov-2s蛋白
<400>24
metphevalpheleuvalleuleuproleuvalserserglncysval
151015
asnleuthrthrargthrglnleuproproalatyrthrasnserphe
202530
thrargglyvaltyrtyrproasplysvalpheargserservalleu
354045
hisserthrglnaspleupheleuprophepheserasnvalthrtrp
505560
phehisalailehisvalserglythrasnglythrlysargpheasp
65707580
asnprovalleupropheasnaspglyvaltyrphealaserthrglu
859095
lysserasnileileargglytrpilepheglythrthrleuaspser
100105110
lysthrglnserleuleuilevalasnasnalathrasnvalvalile
115120125
lysvalcysglupheglnphecysasnasppropheleuglyvaltyr
130135140
tyrhislysasnasnlyssertrpmetgluserglupheargvaltyr
145150155160
serseralaasnasncysthrpheglutyrvalserglnpropheleu
165170175
metaspleugluglylysglnglyasnphelysasnleuarggluphe
180185190
valphelysasnileaspglytyrphelysiletyrserlyshisthr
195200205
proileasnleuvalargaspleuproglnglypheseralaleuglu
210215220
proleuvalaspleuproileglyileasnilethrargpheglnthr
225230235240
leuleualaleuhisargsertyrleuthrproglyaspserserser
245250255
glytrpthralaglyalaalaalatyrtyrvalglytyrleuglnpro
260265270
argthrpheleuleulystyrasngluasnglythrilethraspala
275280285
valaspcysalaleuaspproleusergluthrlyscysthrleulys
290295300
serphethrvalglulysglyiletyrglnthrserasnpheargval
305310315320
glnprothrgluserilevalargpheproasnilethrasnleucys
325330335
propheglygluvalpheasnalathrargphealaservaltyrala
340345350
trpasnarglysargileserasncysvalalaasptyrservalleu
355360365
tyrasnseralaserpheserthrphelyscystyrglyvalserpro
370375380
thrlysleuasnaspleucysphethrasnvaltyralaaspserphe
385390395400
valileargglyaspgluvalargglnilealaproglyglnthrgly
405410415
lysilealaasptyrasntyrlysleuproaspaspphethrglycys
420425430
valilealatrpasnserasnasnleuaspserlysvalglyglyasn
435440445
tyrasntyrleutyrargleuphearglysserasnleulysprophe
450455460
gluargaspileserthrgluiletyrglnalaglyserthrprocys
465470475480
asnglyvalgluglypheasncystyrpheproleuglnsertyrgly
485490495
pheglnprothrasnglyvalglytyrglnprotyrargvalvalval
500505510
leuserphegluleuleuhisalaproalathrvalcysglyprolys
515520525
lysserthrasnleuvallysasnlyscysvalasnpheasnpheasn
530535540
glyleuthrglythrglyvalleuthrgluserasnlyslyspheleu
545550555560
propheglnglnpheglyargaspilealaaspthrthraspalaval
565570575
argaspproglnthrleugluileleuaspilethrprocysserphe
580585590
glyglyvalservalilethrproglythrasnthrserasnglnval
595600605
alavalleutyrglnaspvalasncysthrgluvalprovalalaile
610615620
hisalaaspglnleuthrprothrtrpargvaltyrserthrglyser
625630635640
asnvalpheglnthrargalaglycysleuileglyalagluhisval
645650655
asnasnsertyrglucysaspileproileglyalaglyilecysala
660665670
sertyrglnthrglnthrasnserproglyglyserglyservalala
675680685
serglnserileilealatyrthrmetserleuglyalagluasnser
690695700
valalatyrserasnasnserilealaileprothrasnphethrile
705710715720
servalthrthrgluileleuprovalsermetthrlysthrserval
725730735
aspcysthrmettyrilecysglyaspserthrglucysserasnleu
740745750
leuleuglntyrglyserphecysthrglnleuasnargalaleuthr
755760765
glyilealavalgluglnasplysasnthrglngluvalphealagln
770775780
vallysglnproaspproserlysproserlysargserpheileglu
785790795800
aspleuleupheasnlysvalthrleualaaspalaglypheilelys
805810815
glntyrglyaspcysleuglyaspilealaalaargaspleuilecys
820825830
alaglnlyspheasnglyleuthrvalleuproproleuleuthrasp
835840845
glumetilealaglntyrthrseralaleuleualaglythrilethr
850855860
serglytrpthrpheglyalaglyalaalaleuglnilepropheala
865870875880
metglnmetalatyrargpheasnglyileglyvalthrglnasnval
885890895
leutyrgluasnglnlysleuilealaasnglnpheasnseralaile
900905910
glylysileglnaspserleuserserthralaseralaleuglylys
915920925
leuglnaspvalvalasnglnasnalaglnalaleuasnthrleuval
930935940
lysglnleuserserasnpheglyalaileserservalleuasnasp
945950955960
ileleuserargleuaspprovalglualagluvalglnileasparg
965970975
leuilethrglyargleuglnserleuglnthrtyrvalthrglngln
980985990
leuileargalaalagluileargalaseralaasnleualaalathr
99510001005
lysmetserglucysvalleuglyglnserlysargvalaspphe
101010151020
cysglylysglytyrhisleumetserpheproglnseralapro
102510301035
hisglyvalvalpheleuhisvalthrtyrvalproalaglnglu
104010451050
lysasnphethrthralaproalailecyshisaspglylysala
105510601065
hispheproarggluglyvalphevalserasnglythrhistrp
107010751080
phevalthrglnargasnphetyrgluproglnileilethrthr
108510901095
aspasnthrphevalserglyasncysaspvalvalileglyile
110011051110
valasnasnthrvaltyraspproleuglnprogluleuaspser
111511201125
phelysglugluleuasplystyrphelysasnhisthrserpro
113011351140
aspvalaspleuglyaspileserglyileasnalaservalval
114511501155
asnileglnlysgluileaspargleuasngluvalalalysasn
116011651170
leuasngluserleuileaspleuglngluleuglylystyrglu
117511801185
glntyrilelystrpprotrptyriletrpleuglypheileala
119011951200
glyleuilealailevalmetvalthrilemetleucyscysmet
120512101215
<210>25
<211>1218
<212>prt
<213>人工序列
<220>
<223>重组sars-cov-2s蛋白
<400>25
metphevalpheleuvalleuleuproleuvalserserglncysval
151015
asnleuthrthrargthrglnleuproproalatyrthrasnserphe
202530
thrargglyvaltyrtyrproasplysvalpheargserservalleu
354045
hisserthrglnaspleupheleuprophepheserasnvalthrtrp
505560
phehisalailehisvalserglythrasnglythrlysargpheasp
65707580
asnprovalleupropheasnaspglyvaltyrphealaserthrglu
859095
lysserasnileileargglytrpilepheglythrthrleuaspser
100105110
lysthrglnserleuleuilevalasnasnalathrasnvalvalile
115120125
lysvalcysglupheglnphecysasnasppropheleuglyvaltyr
130135140
tyrhislysasnasnlyssertrpmetgluserglupheargvaltyr
145150155160
serseralaasnasncysthrpheglutyrvalserglnpropheleu
165170175
metaspleugluglylysglnglyasnphelysasnleuarggluphe
180185190
valphelysasnileaspglytyrphelysiletyrserlyshisthr
195200205
proileasnleuvalargaspleuproglnglypheseralaleuglu
210215220
proleuvalaspleuproileglyileasnilethrargpheglnthr
225230235240
leuleualaleuhisargsertyrleuthrproglyaspserserser
245250255
glytrpthralaglyalaalaalatyrtyrvalglytyrleuglnpro
260265270
argthrpheleuleulystyrasngluasnglythrilethraspala
275280285
valaspcysalaleuaspproleusergluthrlyscysthrleulys
290295300
serphethrvalglulysglyiletyrglnthrserasnpheargval
305310315320
glnprothrgluserilevalargpheproasnilethrasnleucys
325330335
propheglygluvalpheasnalathrargphealaservaltyrala
340345350
trpasnarglysargileserasncysvalalaasptyrservalleu
355360365
tyrasnseralaserpheserthrphelyscystyrglyvalserpro
370375380
thrlysleuasnaspleucysphethrasnvaltyralaaspserphe
385390395400
valileargglyaspgluvalargglnilealaproglyglnthrgly
405410415
lysilealaasptyrasntyrlysleuproaspaspphethrglycys
420425430
valilealatrpasnserasnasnleuaspserlysvalglyglyasn
435440445
tyrasntyrleutyrargleuphearglysserasnleulysprophe
450455460
gluargaspileserthrgluiletyrglnalaglyserthrprocys
465470475480
asnglyvalgluglypheasncystyrpheproleuglnsertyrgly
485490495
pheglnprothrasnglyvalglytyrglnprotyrargvalvalval
500505510
leuserphegluleuleuhisalaproalathrvalcysglyprolys
515520525
lysserthrasnleuvallysasnlyscysvalasnpheasnpheasn
530535540
glyleuthrglythrglyvalleuthrgluserasnlyslyspheleu
545550555560
propheglnglnpheglyargaspilealaaspthrthraspalaval
565570575
argaspproglnthrleugluileleuaspilethrprocysserphe
580585590
glyglyvalservalilethrproglythrasnthrserasnglnval
595600605
alavalleutyrglnaspvalasncysthrgluvalprovalalaile
610615620
hisalaaspglnleuthrprothrtrpargvaltyrserthrglyser
625630635640
asnvalpheglnthrargalaglycysleuileglyalagluhisval
645650655
asnasnsertyrglucysaspileproileglyalaglyilecysala
660665670
sertyrglnthrglnthrasnserproglyglyserglyservalala
675680685
serglnserileilealatyrthrmetserleuglyalagluasnser
690695700
valalatyrserasnasnserilealaileprothrasnphethrile
705710715720
servalthrthrgluileleuprovalsermetthrlysthrserval
725730735
aspcysthrmettyrilecysglyaspserthrglucysserasnleu
740745750
leuleuglntyrglyserphecysthrglnleuasnargalaleuthr
755760765
glyilealavalgluglnasplysasnthrglngluvalphealagln
770775780
vallysglnproaspproserlysproserlysargserpheileglu
785790795800
aspleuleupheasnlysvalthrleualaaspalaglypheilelys
805810815
glntyrglyaspcysleuglyaspilealaalaargaspleuilecys
820825830
alaglnlyspheasnglyleuthrvalleuproproleuleuthrasp
835840845
glumetilealaglntyrthrseralaleuleualaglythrilethr
850855860
serglytrpthrpheglyalaglyalaalaleuglnilepropheala
865870875880
metglnmetalatyrargpheasnglyileglyvalthrglnasnval
885890895
leutyrgluasnglnlysleuilealaasnglnpheasnseralaile
900905910
glylysileglnaspserleuserserthralaseralaleuglylys
915920925
leuglnaspvalvalasnglnasnalaglnalaleuasnthrleuval
930935940
lysglnleuserserasnpheglyalaileserservalleuasnasp
945950955960
ileleuserargleuasplysproglualagluvalglnileasparg
965970975
leuilethrglyargleuglnserleuglnthrtyrvalthrglngln
980985990
leuileargalaalagluileargalaseralaasnleualaalathr
99510001005
lysmetserglucysvalleuglyglnserlysargvalaspphe
101010151020
cysglylysglytyrhisleumetserpheproglnseralapro
102510301035
hisglyvalvalpheleuhisvalthrtyrvalproalaglnglu
104010451050
lysasnphethrthralaproalailecyshisaspglylysala
105510601065
hispheproarggluglyvalphevalserasnglythrhistrp
107010751080
phevalthrglnargasnphetyrgluproglnileilethrthr
108510901095
aspasnthrphevalserglyasncysaspvalvalileglyile
110011051110
valasnasnthrvaltyraspproleuglnprogluleuaspser
111511201125
phelysglugluleuasplystyrphelysasnhisthrserpro
113011351140
aspvalaspleuglyaspileserglyileasnalaservalval
114511501155
asnileglnlysgluileaspargleuasngluvalalalysasn
116011651170
leuasngluserleuileaspleuglngluleuglylystyrglu
117511801185
glntyrilelystrpprotrptyriletrpleuglypheileala
119011951200
glyleuilealailevalmetvalthrilemetleucyscysmet
120512101215
<210>26
<211>1218
<212>prt
<213>人工序列
<220>
<223>重组sars-cov-2s蛋白
<400>26
metphevalpheleuvalleuleuproleuvalserserglncysval
151015
asnleuthrthrargthrglnleuproproalatyrthrasnserphe
202530
thrargglyvaltyrtyrproasplysvalpheargserservalleu
354045
hisserthrglnaspleupheleuprophepheserasnvalthrtrp
505560
phehisalailehisvalserglythrasnglythrlysargpheasp
65707580
asnprovalleupropheasnaspglyvaltyrphealaserthrglu
859095
lysserasnileileargglytrpilepheglythrthrleuaspser
100105110
lysthrglnserleuleuilevalasnasnalathrasnvalvalile
115120125
lysvalcysglupheglnphecysasnasppropheleuglyvaltyr
130135140
tyrhislysasnasnlyssertrpmetgluserglupheargvaltyr
145150155160
serseralaasnasncysthrpheglutyrvalserglnpropheleu
165170175
metaspleugluglylysglnglyasnphelysasnleuarggluphe
180185190
valphelysasnileaspglytyrphelysiletyrserlyshisthr
195200205
proileasnleuvalargaspleuproglnglypheseralaleuglu
210215220
proleuvalaspleuproileglyileasnilethrargpheglnthr
225230235240
leuleualaleuhisargsertyrleuthrproglyaspserserser
245250255
glytrpthralaglyalaalaalatyrtyrvalglytyrleuglnpro
260265270
argthrpheleuleulystyrasngluasnglythrilethraspala
275280285
valaspcysalaleuaspproleusergluthrlyscysthrleulys
290295300
serphethrvalglulysglyiletyrglnthrserasnpheargval
305310315320
glnprothrgluserilevalargpheproasnilethrasnleucys
325330335
propheglygluvalpheasnalathrargphealaservaltyrala
340345350
trpasnarglysargileserasncysvalalaasptyrservalleu
355360365
tyrasnseralaserpheserthrphelyscystyrglyvalserpro
370375380
thrlysleuasnaspleucysphethrasnvaltyralaaspserphe
385390395400
valileargglyaspgluvalargglnilealaproglyglnthrgly
405410415
lysilealaasptyrasntyrlysleuproaspaspphethrglycys
420425430
valilealatrpasnserasnasnleuaspserlysvalglyglyasn
435440445
tyrasntyrleutyrargleuphearglysserasnleulysprophe
450455460
gluargaspileserthrgluiletyrglnalaglyserthrprocys
465470475480
asnglyvalgluglypheasncystyrpheproleuglnsertyrgly
485490495
pheglnprothrasnglyvalglytyrglnprotyrargvalvalval
500505510
leuserphegluleuleuhisalaproalathrvalcysglyprolys
515520525
lysserthrasnleuvallysasnlyscysvalasnpheasnpheasn
530535540
glyleuthrglythrglyvalleuthrgluserasnlyslyspheleu
545550555560
propheglnglnpheglyargaspilealaaspthrthraspalaval
565570575
argaspproglnthrleugluileleuaspilethrprocysserphe
580585590
glyglyvalservalilethrproglythrasnthrserasnglnval
595600605
alavalleutyrglnaspvalasncysthrgluvalprovalalaile
610615620
hisalaaspglnleuthrprothrtrpargvaltyrserthrglyser
625630635640
asnvalpheglnthrargalaglycysleuileglyalagluhisval
645650655
asnasnsertyrglucysaspileproileglyalaglyilecysala
660665670
sertyrglnthrglnthrasnserproglyglyserglyservalala
675680685
serglnserileilealatyrthrmetserleuglyalagluasnser
690695700
valalatyrserasnasnserilealaileprothrasnphethrile
705710715720
servalthrthrgluileleuprovalsermetthrlysthrserval
725730735
aspcysthrmettyrilecysglyaspserthrglucysserasnleu
740745750
leuleuglntyrglyserphecysthrglnleuasnargalaleuthr
755760765
glyilealavalgluglnasplysasnthrglngluvalphealagln
770775780
vallysglnproaspproserlysproserlysargserpheileglu
785790795800
aspleuleupheasnlysvalthrleualaaspalaglypheilelys
805810815
glntyrglyaspcysleuglyaspilealaalaargaspleuilecys
820825830
alaglnlyspheasnglyleuthrvalleuproproleuleuthrasp
835840845
glumetilealaglntyrthrseralaleuleualaglythrilethr
850855860
serglytrpthrpheglyalaglyalaalaleuglnilepropheala
865870875880
metglnmetalatyrargpheasnglyileglyvalthrglnasnval
885890895
leutyrgluasnglnlysleuilealaasnglnpheasnseralaile
900905910
glylysileglnaspserleuserserthralaseralaleuglylys
915920925
leuglnaspvalvalasnglnasnalaglnalaleuasnthrleuval
930935940
lysglnleuserserasnpheglyalaileserservalleuasnasp
945950955960
ileleuserargleuaspproproglualagluvalglnileasparg
965970975
leuilethrglyargleuglnserleuglnthrtyrvalthrglngln
980985990
leuileargalaalagluileargalaseralaasnleualaalathr
99510001005
lysmetserglucysvalleuglyglnserlysargvalaspphe
101010151020
cysglylysglytyrhisleumetserpheproglnseralapro
102510301035
hisglyvalvalpheleuhisvalthrtyrvalproalaglnglu
104010451050
lysasnphethrthralaproalailecyshisaspglylysala
105510601065
hispheproarggluglyvalphevalserasnglythrhistrp
107010751080
phevalthrglnargasnphetyrgluproglnileilethrthr
108510901095
aspasnthrphevalserglyasncysaspvalvalileglyile
110011051110
valasnasnthrvaltyraspproleuglnprogluleuaspser
111511201125
phelysglugluleuasplystyrphelysasnhisthrserpro
113011351140
aspvalaspleuglyaspileserglyileasnalaservalval
114511501155
asnileglnlysgluileaspargleuasngluvalalalysasn
116011651170
leuasngluserleuileaspleuglngluleuglylystyrglu
117511801185
glntyrilelystrpprotrptyriletrpleuglypheileala
119011951200
glyleuilealailevalmetvalthrilemetleucyscysmet
120512101215
<210>27
<211>1213
<212>prt
<213>人工序列
<220>
<223>重组sars-cov-2s蛋白
<400>27
metphevalpheleuvalleuleuproleuvalserserglncysval
151015
asnleuthrthrargthrglnleuproproalatyrthrasnserphe
202530
thrargglyvaltyrtyrproasplysvalpheargserservalleu
354045
hisserthrglnaspleupheleuprophepheserasnvalthrtrp
505560
phehisalailehisvalserglythrasnglythrlysargpheasp
65707580
asnprovalleupropheasnaspglyvaltyrphealaserthrglu
859095
lysserasnileileargglytrpilepheglythrthrleuaspser
100105110
lysthrglnserleuleuilevalasnasnalathrasnvalvalile
115120125
lysvalcysglupheglnphecysasnasppropheleuglyvaltyr
130135140
tyrhislysasnasnlyssertrpmetgluserglupheargvaltyr
145150155160
serseralaasnasncysthrpheglutyrvalserglnpropheleu
165170175
metaspleugluglylysglnglyasnphelysasnleuarggluphe
180185190
valphelysasnileaspglytyrphelysiletyrserlyshisthr
195200205
proileasnleuvalargaspleuproglnglypheseralaleuglu
210215220
proleuvalaspleuproileglyileasnilethrargpheglnthr
225230235240
leuleualaleuhisargsertyrleuthrproglyaspserserser
245250255
glytrpthralaglyalaalaalatyrtyrvalglytyrleuglnpro
260265270
argthrpheleuleulystyrasngluasnglythrilethraspala
275280285
valaspcysalaleuaspproleusergluthrlyscysthrleulys
290295300
serphethrvalglulysglyiletyrglnthrserasnpheargval
305310315320
glnprothrgluserilevalargpheproasnilethrasnleucys
325330335
propheglygluvalpheasnalathrargphealaservaltyrala
340345350
trpasnarglysargileserasncysvalalaasptyrservalleu
355360365
tyrasnseralaserpheserthrphelyscystyrglyvalserpro
370375380
thrlysleuasnaspleucysphethrasnvaltyralaaspserphe
385390395400
valileargglyaspgluvalargglnilealaproglyglnthrgly
405410415
lysilealaasptyrasntyrlysleuproaspaspphethrglycys
420425430
valilealatrpasnserasnasnleuaspserlysvalglyglyasn
435440445
tyrasntyrleutyrargleuphearglysserasnleulysprophe
450455460
gluargaspileserthrgluiletyrglnalaglyserthrprocys
465470475480
asnglyvalgluglypheasncystyrpheproleuglnsertyrgly
485490495
pheglnprothrasnglyvalglytyrglnprotyrargvalvalval
500505510
leuserphegluleuleuhisalaproalathrvalcysglyprolys
515520525
lysserthrasnleuvallysasnlyscysvalasnpheasnpheasn
530535540
glyleuthrglythrglyvalleuthrgluserasnlyslyspheleu
545550555560
propheglnglnpheglyargaspilealaaspthrthraspalaval
565570575
argaspproglnthrleugluileleuaspilethrprocysserphe
580585590
glyglyvalservalilethrproglythrasnthrserasnglnval
595600605
alavalleutyrglnaspvalasncysthrgluvalprovalalaile
610615620
hisalaaspglnleuthrprothrtrpargvaltyrserthrglyser
625630635640
asnvalpheglnthrargalaglycysleuileglyalagluhisval
645650655
asnasnsertyrglucysaspileproileglyalaglyilecysala
660665670
sertyrglnthrglnthrasnserproglyglyserglyservalala
675680685
serglnserileilealatyrthrmetserleuglyalagluasnser
690695700
valalatyrserasnasnserilealaileprothrasnphethrile
705710715720
servalthrthrgluileleuprovalsermetthrlysthrserval
725730735
aspcysthrmettyrilecysglyaspserthrglucysserasnleu
740745750
leuleuglntyrglyserphecysthrglnleuasnargalaleuthr
755760765
glyilealavalgluglnasplysasnthrglngluvalphealagln
770775780
vallysglniletyrlysthrproproilelysasppheglyglyphe
785790795800
asnpheserglnileleuproaspproserlysproseralaasnser
805810815
pheilegluaspleuleupheasnlysvalthrleualaaspalagly
820825830
pheilelysglntyrglyaspcysleuglyaspilealaalaargasp
835840845
leuilecysalaglnlyspheasnglyleuthrvalleuproproleu
850855860
leuthraspglumetilealaglntyrthrseralaleuleualagly
865870875880
thrilethrserglytrpthrpheglyalaglyalaalaleuglnile
885890895
prophealametglnmetalatyrargpheasnglyileglyvalthr
900905910
glnasnvalleutyrgluasnglnlysleuilealaasnglnpheasn
915920925
seralaileglylysileglnaspserleuserserthralaserala
930935940
leuglylysleuglnaspvalvalasnglnasnalaglnalaleuasn
945950955960
thrleuvallysglnleuserserasnpheglyalaileserserval
965970975
leuasnaspileleuserargleuaspprovalglualagluvalgln
980985990
ileaspargleuilethrglyargleuglnserleuglnthrtyrval
99510001005
thrglnglnleuileargalaalagluileargalaseralaasn
101010151020
leualaalathrlysmetserglucysvalleuglyglnserlys
102510301035
argvalaspphecysglylysglytyrhisleumetserphepro
104010451050
glnseralaprohisglyvalvalpheleuhisvalthrtyrval
105510601065
proalaglnglulysasnphethrthralaproalailecyshis
107010751080
aspglylysalahispheproarggluglyvalphevalserasn
108510901095
glythrhistrpphevalthrglnargasnphetyrgluprogln
110011051110
ileilethrthraspasnthrphevalserglyasncysaspval
111511201125
valileglyilevalasnasnthrvaltyraspproleuglnpro
113011351140
gluleuaspserphelysglugluleuasplystyrphelysasn
114511501155
histhrserproaspvalaspleuglyaspileserglyileasn
116011651170
alaservalvalasnileglnlysgluileaspargleuasnglu
117511801185
valalalysasnleuasngluserleuileaspleuglngluleu
119011951200
glylystyrgluglntyrilelystrppro
12051210
<210>28
<211>1213
<212>prt
<213>人工序列
<220>
<223>重组sars-cov-2s蛋白
<400>28
metphevalpheleuvalleuleuproleuvalserserglncysval
151015
asnleuthrthrargthrglnleuproproalatyrthrasnserphe
202530
thrargglyvaltyrtyrproasplysvalpheargserservalleu
354045
hisserthrglnaspleupheleuprophepheserasnvalthrtrp
505560
phehisalailehisvalserglythrasnglythrlysargpheasp
65707580
asnprovalleupropheasnaspglyvaltyrphealaserthrglu
859095
lysserasnileileargglytrpilepheglythrthrleuaspser
100105110
lysthrglnserleuleuilevalasnasnalathrasnvalvalile
115120125
lysvalcysglupheglnphecysasnasppropheleuglyvaltyr
130135140
tyrhislysasnasnlyssertrpmetgluserglupheargvaltyr
145150155160
serseralaasnasncysthrpheglutyrvalserglnpropheleu
165170175
metaspleugluglylysglnglyasnphelysasnleuarggluphe
180185190
valphelysasnileaspglytyrphelysiletyrserlyshisthr
195200205
proileasnleuvalargaspleuproglnglypheseralaleuglu
210215220
proleuvalaspleuproileglyileasnilethrargpheglnthr
225230235240
leuleualaleuhisargsertyrleuthrproglyaspserserser
245250255
glytrpthralaglyalaalaalatyrtyrvalglytyrleuglnpro
260265270
argthrpheleuleulystyrasngluasnglythrilethraspala
275280285
valaspcysalaleuaspproleusergluthrlyscysthrleulys
290295300
serphethrvalglulysglyiletyrglnthrserasnpheargval
305310315320
glnprothrgluserilevalargpheproasnilethrasnleucys
325330335
propheglygluvalpheasnalathrargphealaservaltyrala
340345350
trpasnarglysargileserasncysvalalaasptyrservalleu
355360365
tyrasnseralaserpheserthrphelyscystyrglyvalserpro
370375380
thrlysleuasnaspleucysphethrasnvaltyralaaspserphe
385390395400
valileargglyaspgluvalargglnilealaproglyglnthrgly
405410415
lysilealaasptyrasntyrlysleuproaspaspphethrglycys
420425430
valilealatrpasnserasnasnleuaspserlysvalglyglyasn
435440445
tyrasntyrleutyrargleuphearglysserasnleulysprophe
450455460
gluargaspileserthrgluiletyrglnalaglyserthrprocys
465470475480
asnglyvalgluglypheasncystyrpheproleuglnsertyrgly
485490495
pheglnprothrasnglyvalglytyrglnprotyrargvalvalval
500505510
leuserphegluleuleuhisalaproalathrvalcysglyprolys
515520525
lysserthrasnleuvallysasnlyscysvalasnpheasnpheasn
530535540
glyleuthrglythrglyvalleuthrgluserasnlyslyspheleu
545550555560
propheglnglnpheglyargaspilealaaspthrthraspalaval
565570575
argaspproglnthrleugluileleuaspilethrprocysserphe
580585590
glyglyvalservalilethrproglythrasnthrserasnglnval
595600605
alavalleutyrglnaspvalasncysthrgluvalprovalalaile
610615620
hisalaaspglnleuthrprothrtrpargvaltyrserthrglyser
625630635640
asnvalpheglnthrargalaglycysleuileglyalagluhisval
645650655
asnasnsertyrglucysaspileproileglyalaglyilecysala
660665670
sertyrglnthrglnthrasnserproglyglyserglyservalala
675680685
serglnserileilealatyrthrmetserleuglyalagluasnser
690695700
valalatyrserasnasnserilealaileprothrasnphethrile
705710715720
servalthrthrgluileleuprovalsermetthrlysthrserval
725730735
aspcysthrmettyrilecysglyaspserthrglucysserasnleu
740745750
leuleuglntyrglyserphecysthrglnleuasnargalaleuthr
755760765
glyilealavalgluglnasplysasnthrglngluvalphealagln
770775780
vallysglniletyrlysthrproproilelysasppheglyglyphe
785790795800
asnpheserglnileleuproaspproserlysproseralaasnser
805810815
pheilegluaspleuleupheasnlysvalthrleualaaspalagly
820825830
pheilelysglntyrglyaspcysleuglyaspilealaalaargasp
835840845
leuilecysalaglnlyspheasnglyleuthrvalleuproproleu
850855860
leuthraspglumetilealaglntyrthrseralaleuleualagly
865870875880
thrilethrserglytrpthrpheglyalaglyalaalaleuglnile
885890895
prophealametglnmetalatyrargpheasnglyileglyvalthr
900905910
glnasnvalleutyrgluasnglnlysleuilealaasnglnpheasn
915920925
seralaileglylysileglnaspserleuserserthralaserala
930935940
leuglylysleuglnaspvalvalasnglnasnalaglnalaleuasn
945950955960
thrleuvallysglnleuserserasnpheglyalaileserserval
965970975
leuasnaspileleuserargleuasplysproglualagluvalgln
980985990
ileaspargleuilethrglyargleuglnserleuglnthrtyrval
99510001005
thrglnglnleuileargalaalagluileargalaseralaasn
101010151020
leualaalathrlysmetserglucysvalleuglyglnserlys
102510301035
argvalaspphecysglylysglytyrhisleumetserphepro
104010451050
glnseralaprohisglyvalvalpheleuhisvalthrtyrval
105510601065
proalaglnglulysasnphethrthralaproalailecyshis
107010751080
aspglylysalahispheproarggluglyvalphevalserasn
108510901095
glythrhistrpphevalthrglnargasnphetyrgluprogln
110011051110
ileilethrthraspasnthrphevalserglyasncysaspval
111511201125
valileglyilevalasnasnthrvaltyraspproleuglnpro
113011351140
gluleuaspserphelysglugluleuasplystyrphelysasn
114511501155
histhrserproaspvalaspleuglyaspileserglyileasn
116011651170
alaservalvalasnileglnlysgluileaspargleuasnglu
117511801185
valalalysasnleuasngluserleuileaspleuglngluleu
119011951200
glylystyrgluglntyrilelystrppro
12051210
<210>29
<211>1213
<212>prt
<213>人工序列
<220>
<223>重组sars-cov-2s蛋白
<400>29
metphevalpheleuvalleuleuproleuvalserserglncysval
151015
asnleuthrthrargthrglnleuproproalatyrthrasnserphe
202530
thrargglyvaltyrtyrproasplysvalpheargserservalleu
354045
hisserthrglnaspleupheleuprophepheserasnvalthrtrp
505560
phehisalailehisvalserglythrasnglythrlysargpheasp
65707580
asnprovalleupropheasnaspglyvaltyrphealaserthrglu
859095
lysserasnileileargglytrpilepheglythrthrleuaspser
100105110
lysthrglnserleuleuilevalasnasnalathrasnvalvalile
115120125
lysvalcysglupheglnphecysasnasppropheleuglyvaltyr
130135140
tyrhislysasnasnlyssertrpmetgluserglupheargvaltyr
145150155160
serseralaasnasncysthrpheglutyrvalserglnpropheleu
165170175
metaspleugluglylysglnglyasnphelysasnleuarggluphe
180185190
valphelysasnileaspglytyrphelysiletyrserlyshisthr
195200205
proileasnleuvalargaspleuproglnglypheseralaleuglu
210215220
proleuvalaspleuproileglyileasnilethrargpheglnthr
225230235240
leuleualaleuhisargsertyrleuthrproglyaspserserser
245250255
glytrpthralaglyalaalaalatyrtyrvalglytyrleuglnpro
260265270
argthrpheleuleulystyrasngluasnglythrilethraspala
275280285
valaspcysalaleuaspproleusergluthrlyscysthrleulys
290295300
serphethrvalglulysglyiletyrglnthrserasnpheargval
305310315320
glnprothrgluserilevalargpheproasnilethrasnleucys
325330335
propheglygluvalpheasnalathrargphealaservaltyrala
340345350
trpasnarglysargileserasncysvalalaasptyrservalleu
355360365
tyrasnseralaserpheserthrphelyscystyrglyvalserpro
370375380
thrlysleuasnaspleucysphethrasnvaltyralaaspserphe
385390395400
valileargglyaspgluvalargglnilealaproglyglnthrgly
405410415
lysilealaasptyrasntyrlysleuproaspaspphethrglycys
420425430
valilealatrpasnserasnasnleuaspserlysvalglyglyasn
435440445
tyrasntyrleutyrargleuphearglysserasnleulysprophe
450455460
gluargaspileserthrgluiletyrglnalaglyserthrprocys
465470475480
asnglyvalgluglypheasncystyrpheproleuglnsertyrgly
485490495
pheglnprothrasnglyvalglytyrglnprotyrargvalvalval
500505510
leuserphegluleuleuhisalaproalathrvalcysglyprolys
515520525
lysserthrasnleuvallysasnlyscysvalasnpheasnpheasn
530535540
glyleuthrglythrglyvalleuthrgluserasnlyslyspheleu
545550555560
propheglnglnpheglyargaspilealaaspthrthraspalaval
565570575
argaspproglnthrleugluileleuaspilethrprocysserphe
580585590
glyglyvalservalilethrproglythrasnthrserasnglnval
595600605
alavalleutyrglnaspvalasncysthrgluvalprovalalaile
610615620
hisalaaspglnleuthrprothrtrpargvaltyrserthrglyser
625630635640
asnvalpheglnthrargalaglycysleuileglyalagluhisval
645650655
asnasnsertyrglucysaspileproileglyalaglyilecysala
660665670
sertyrglnthrglnthrasnserproglyglyserglyservalala
675680685
serglnserileilealatyrthrmetserleuglyalagluasnser
690695700
valalatyrserasnasnserilealaileprothrasnphethrile
705710715720
servalthrthrgluileleuprovalsermetthrlysthrserval
725730735
aspcysthrmettyrilecysglyaspserthrglucysserasnleu
740745750
leuleuglntyrglyserphecysthrglnleuasnargalaleuthr
755760765
glyilealavalgluglnasplysasnthrglngluvalphealagln
770775780
vallysglniletyrlysthrproproilelysasppheglyglyphe
785790795800
asnpheserglnileleuproaspproserlysproseralaasnser
805810815
pheilegluaspleuleupheasnlysvalthrleualaaspalagly
820825830
pheilelysglntyrglyaspcysleuglyaspilealaalaargasp
835840845
leuilecysalaglnlyspheasnglyleuthrvalleuproproleu
850855860
leuthraspglumetilealaglntyrthrseralaleuleualagly
865870875880
thrilethrserglytrpthrpheglyalaglyalaalaleuglnile
885890895
prophealametglnmetalatyrargpheasnglyileglyvalthr
900905910
glnasnvalleutyrgluasnglnlysleuilealaasnglnpheasn
915920925
seralaileglylysileglnaspserleuserserthralaserala
930935940
leuglylysleuglnaspvalvalasnglnasnalaglnalaleuasn
945950955960
thrleuvallysglnleuserserasnpheglyalaileserserval
965970975
leuasnaspileleuserargleuaspproproglualagluvalgln
980985990
ileaspargleuilethrglyargleuglnserleuglnthrtyrval
99510001005
thrglnglnleuileargalaalagluileargalaseralaasn
101010151020
leualaalathrlysmetserglucysvalleuglyglnserlys
102510301035
argvalaspphecysglylysglytyrhisleumetserphepro
104010451050
glnseralaprohisglyvalvalpheleuhisvalthrtyrval
105510601065
proalaglnglulysasnphethrthralaproalailecyshis
107010751080
aspglylysalahispheproarggluglyvalphevalserasn
108510901095
glythrhistrpphevalthrglnargasnphetyrgluprogln
110011051110
ileilethrthraspasnthrphevalserglyasncysaspval
111511201125
valileglyilevalasnasnthrvaltyraspproleuglnpro
113011351140
gluleuaspserphelysglugluleuasplystyrphelysasn
114511501155
histhrserproaspvalaspleuglyaspileserglyileasn
116011651170
alaservalvalasnileglnlysgluileaspargleuasnglu
117511801185
valalalysasnleuasngluserleuileaspleuglngluleu
119011951200
glylystyrgluglntyrilelystrppro
12051210
<210>30
<211>1194
<212>prt
<213>人工序列
<220>
<223>重组sars-cov-2s蛋白
<400>30
metphevalpheleuvalleuleuproleuvalserserglncysval
151015
asnleuthrthrargthrglnleuproproalatyrthrasnserphe
202530
thrargglyvaltyrtyrproasplysvalpheargserservalleu
354045
hisserthrglnaspleupheleuprophepheserasnvalthrtrp
505560
phehisalailehisvalserglythrasnglythrlysargpheasp
65707580
asnprovalleupropheasnaspglyvaltyrphealaserthrglu
859095
lysserasnileileargglytrpilepheglythrthrleuaspser
100105110
lysthrglnserleuleuilevalasnasnalathrasnvalvalile
115120125
lysvalcysglupheglnphecysasnasppropheleuglyvaltyr
130135140
tyrhislysasnasnlyssertrpmetgluserglupheargvaltyr
145150155160
serseralaasnasncysthrpheglutyrvalserglnpropheleu
165170175
metaspleugluglylysglnglyasnphelysasnleuarggluphe
180185190
valphelysasnileaspglytyrphelysiletyrserlyshisthr
195200205
proileasnleuvalargaspleuproglnglypheseralaleuglu
210215220
proleuvalaspleuproileglyileasnilethrargpheglnthr
225230235240
leuleualaleuhisargsertyrleuthrproglyaspserserser
245250255
glytrpthralaglyalaalaalatyrtyrvalglytyrleuglnpro
260265270
argthrpheleuleulystyrasngluasnglythrilethraspala
275280285
valaspcysalaleuaspproleusergluthrlyscysthrleulys
290295300
serphethrvalglulysglyiletyrglnthrserasnpheargval
305310315320
glnprothrgluserilevalargpheproasnilethrasnleucys
325330335
propheglygluvalpheasnalathrargphealaservaltyrala
340345350
trpasnarglysargileserasncysvalalaasptyrservalleu
355360365
tyrasnseralaserpheserthrphelyscystyrglyvalserpro
370375380
thrlysleuasnaspleucysphethrasnvaltyralaaspserphe
385390395400
valileargglyaspgluvalargglnilealaproglyglnthrgly
405410415
lysilealaasptyrasntyrlysleuproaspaspphethrglycys
420425430
valilealatrpasnserasnasnleuaspserlysvalglyglyasn
435440445
tyrasntyrleutyrargleuphearglysserasnleulysprophe
450455460
gluargaspileserthrgluiletyrglnalaglyserthrprocys
465470475480
asnglyvalgluglypheasncystyrpheproleuglnsertyrgly
485490495
pheglnprothrasnglyvalglytyrglnprotyrargvalvalval
500505510
leuserphegluleuleuhisalaproalathrvalcysglyprolys
515520525
lysserthrasnleuvallysasnlyscysvalasnpheasnpheasn
530535540
glyleuthrglythrglyvalleuthrgluserasnlyslyspheleu
545550555560
propheglnglnpheglyargaspilealaaspthrthraspalaval
565570575
argaspproglnthrleugluileleuaspilethrprocysserphe
580585590
glyglyvalservalilethrproglythrasnthrserasnglnval
595600605
alavalleutyrglnaspvalasncysthrgluvalprovalalaile
610615620
hisalaaspglnleuthrprothrtrpargvaltyrserthrglyser
625630635640
asnvalpheglnthrargalaglycysleuileglyalagluhisval
645650655
asnasnsertyrglucysaspileproileglyalaglyilecysala
660665670
sertyrglnthrglnthrasnserproglyglyserglyservalala
675680685
serglnserileilealatyrthrmetserleuglyalagluasnser
690695700
valalatyrserasnasnserilealaileprothrasnphethrile
705710715720
servalthrthrgluileleuprovalsermetthrlysthrserval
725730735
aspcysthrmettyrilecysglyaspserthrglucysserasnleu
740745750
leuleuglntyrglyserphecysthrglnleuasnargalaleuthr
755760765
glyilealavalgluglnasplysasnthrglngluvalphealagln
770775780
vallysglnproaspproserlysproseralaasnserpheileglu
785790795800
aspleuleupheasnlysvalthrleualaaspalaglypheilelys
805810815
glntyrglyaspcysleuglyaspilealaalaargaspleuilecys
820825830
alaglnlyspheasnglyleuthrvalleuproproleuleuthrasp
835840845
glumetilealaglntyrthrseralaleuleualaglythrilethr
850855860
serglytrpthrpheglyalaglyalaalaleuglnilepropheala
865870875880
metglnmetalatyrargpheasnglyileglyvalthrglnasnval
885890895
leutyrgluasnglnlysleuilealaasnglnpheasnseralaile
900905910
glylysileglnaspserleuserserthralaseralaleuglylys
915920925
leuglnaspvalvalasnglnasnalaglnalaleuasnthrleuval
930935940
lysglnleuserserasnpheglyalaileserservalleuasnasp
945950955960
ileleuserargleuaspprovalglualagluvalglnileasparg
965970975
leuilethrglyargleuglnserleuglnthrtyrvalthrglngln
980985990
leuileargalaalagluileargalaseralaasnleualaalathr
99510001005
lysmetserglucysvalleuglyglnserlysargvalaspphe
101010151020
cysglylysglytyrhisleumetserpheproglnseralapro
102510301035
hisglyvalvalpheleuhisvalthrtyrvalproalaglnglu
104010451050
lysasnphethrthralaproalailecyshisaspglylysala
105510601065
hispheproarggluglyvalphevalserasnglythrhistrp
107010751080
phevalthrglnargasnphetyrgluproglnileilethrthr
108510901095
aspasnthrphevalserglyasncysaspvalvalileglyile
110011051110
valasnasnthrvaltyraspproleuglnprogluleuaspser
111511201125
phelysglugluleuasplystyrphelysasnhisthrserpro
113011351140
aspvalaspleuglyaspileserglyileasnalaservalval
114511501155
asnileglnlysgluileaspargleuasngluvalalalysasn
116011651170
leuasngluserleuileaspleuglngluleuglylystyrglu
117511801185
glntyrilelystrppro
1190
<210>31
<211>1194
<212>prt
<213>人工序列
<220>
<223>重组sars-cov-2s蛋白
<400>31
metphevalpheleuvalleuleuproleuvalserserglncysval
151015
asnleuthrthrargthrglnleuproproalatyrthrasnserphe
202530
thrargglyvaltyrtyrproasplysvalpheargserservalleu
354045
hisserthrglnaspleupheleuprophepheserasnvalthrtrp
505560
phehisalailehisvalserglythrasnglythrlysargpheasp
65707580
asnprovalleupropheasnaspglyvaltyrphealaserthrglu
859095
lysserasnileileargglytrpilepheglythrthrleuaspser
100105110
lysthrglnserleuleuilevalasnasnalathrasnvalvalile
115120125
lysvalcysglupheglnphecysasnasppropheleuglyvaltyr
130135140
tyrhislysasnasnlyssertrpmetgluserglupheargvaltyr
145150155160
serseralaasnasncysthrpheglutyrvalserglnpropheleu
165170175
metaspleugluglylysglnglyasnphelysasnleuarggluphe
180185190
valphelysasnileaspglytyrphelysiletyrserlyshisthr
195200205
proileasnleuvalargaspleuproglnglypheseralaleuglu
210215220
proleuvalaspleuproileglyileasnilethrargpheglnthr
225230235240
leuleualaleuhisargsertyrleuthrproglyaspserserser
245250255
glytrpthralaglyalaalaalatyrtyrvalglytyrleuglnpro
260265270
argthrpheleuleulystyrasngluasnglythrilethraspala
275280285
valaspcysalaleuaspproleusergluthrlyscysthrleulys
290295300
serphethrvalglulysglyiletyrglnthrserasnpheargval
305310315320
glnprothrgluserilevalargpheproasnilethrasnleucys
325330335
propheglygluvalpheasnalathrargphealaservaltyrala
340345350
trpasnarglysargileserasncysvalalaasptyrservalleu
355360365
tyrasnseralaserpheserthrphelyscystyrglyvalserpro
370375380
thrlysleuasnaspleucysphethrasnvaltyralaaspserphe
385390395400
valileargglyaspgluvalargglnilealaproglyglnthrgly
405410415
lysilealaasptyrasntyrlysleuproaspaspphethrglycys
420425430
valilealatrpasnserasnasnleuaspserlysvalglyglyasn
435440445
tyrasntyrleutyrargleuphearglysserasnleulysprophe
450455460
gluargaspileserthrgluiletyrglnalaglyserthrprocys
465470475480
asnglyvalgluglypheasncystyrpheproleuglnsertyrgly
485490495
pheglnprothrasnglyvalglytyrglnprotyrargvalvalval
500505510
leuserphegluleuleuhisalaproalathrvalcysglyprolys
515520525
lysserthrasnleuvallysasnlyscysvalasnpheasnpheasn
530535540
glyleuthrglythrglyvalleuthrgluserasnlyslyspheleu
545550555560
propheglnglnpheglyargaspilealaaspthrthraspalaval
565570575
argaspproglnthrleugluileleuaspilethrprocysserphe
580585590
glyglyvalservalilethrproglythrasnthrserasnglnval
595600605
alavalleutyrglnaspvalasncysthrgluvalprovalalaile
610615620
hisalaaspglnleuthrprothrtrpargvaltyrserthrglyser
625630635640
asnvalpheglnthrargalaglycysleuileglyalagluhisval
645650655
asnasnsertyrglucysaspileproileglyalaglyilecysala
660665670
sertyrglnthrglnthrasnserproglyglyserglyservalala
675680685
serglnserileilealatyrthrmetserleuglyalagluasnser
690695700
valalatyrserasnasnserilealaileprothrasnphethrile
705710715720
servalthrthrgluileleuprovalsermetthrlysthrserval
725730735
aspcysthrmettyrilecysglyaspserthrglucysserasnleu
740745750
leuleuglntyrglyserphecysthrglnleuasnargalaleuthr
755760765
glyilealavalgluglnasplysasnthrglngluvalphealagln
770775780
vallysglnproaspproserlysproseralaasnserpheileglu
785790795800
aspleuleupheasnlysvalthrleualaaspalaglypheilelys
805810815
glntyrglyaspcysleuglyaspilealaalaargaspleuilecys
820825830
alaglnlyspheasnglyleuthrvalleuproproleuleuthrasp
835840845
glumetilealaglntyrthrseralaleuleualaglythrilethr
850855860
serglytrpthrpheglyalaglyalaalaleuglnilepropheala
865870875880
metglnmetalatyrargpheasnglyileglyvalthrglnasnval
885890895
leutyrgluasnglnlysleuilealaasnglnpheasnseralaile
900905910
glylysileglnaspserleuserserthralaseralaleuglylys
915920925
leuglnaspvalvalasnglnasnalaglnalaleuasnthrleuval
930935940
lysglnleuserserasnpheglyalaileserservalleuasnasp
945950955960
ileleuserargleuasplysproglualagluvalglnileasparg
965970975
leuilethrglyargleuglnserleuglnthrtyrvalthrglngln
980985990
leuileargalaalagluileargalaseralaasnleualaalathr
99510001005
lysmetserglucysvalleuglyglnserlysargvalaspphe
101010151020
cysglylysglytyrhisleumetserpheproglnseralapro
102510301035
hisglyvalvalpheleuhisvalthrtyrvalproalaglnglu
104010451050
lysasnphethrthralaproalailecyshisaspglylysala
105510601065
hispheproarggluglyvalphevalserasnglythrhistrp
107010751080
phevalthrglnargasnphetyrgluproglnileilethrthr
108510901095
aspasnthrphevalserglyasncysaspvalvalileglyile
110011051110
valasnasnthrvaltyraspproleuglnprogluleuaspser
111511201125
phelysglugluleuasplystyrphelysasnhisthrserpro
113011351140
aspvalaspleuglyaspileserglyileasnalaservalval
114511501155
asnileglnlysgluileaspargleuasngluvalalalysasn
116011651170
leuasngluserleuileaspleuglngluleuglylystyrglu
117511801185
glntyrilelystrppro
1190
<210>32
<211>1194
<212>prt
<213>人工序列
<220>
<223>重组sars-cov-2s蛋白
<400>32
metphevalpheleuvalleuleuproleuvalserserglncysval
151015
asnleuthrthrargthrglnleuproproalatyrthrasnserphe
202530
thrargglyvaltyrtyrproasplysvalpheargserservalleu
354045
hisserthrglnaspleupheleuprophepheserasnvalthrtrp
505560
phehisalailehisvalserglythrasnglythrlysargpheasp
65707580
asnprovalleupropheasnaspglyvaltyrphealaserthrglu
859095
lysserasnileileargglytrpilepheglythrthrleuaspser
100105110
lysthrglnserleuleuilevalasnasnalathrasnvalvalile
115120125
lysvalcysglupheglnphecysasnasppropheleuglyvaltyr
130135140
tyrhislysasnasnlyssertrpmetgluserglupheargvaltyr
145150155160
serseralaasnasncysthrpheglutyrvalserglnpropheleu
165170175
metaspleugluglylysglnglyasnphelysasnleuarggluphe
180185190
valphelysasnileaspglytyrphelysiletyrserlyshisthr
195200205
proileasnleuvalargaspleuproglnglypheseralaleuglu
210215220
proleuvalaspleuproileglyileasnilethrargpheglnthr
225230235240
leuleualaleuhisargsertyrleuthrproglyaspserserser
245250255
glytrpthralaglyalaalaalatyrtyrvalglytyrleuglnpro
260265270
argthrpheleuleulystyrasngluasnglythrilethraspala
275280285
valaspcysalaleuaspproleusergluthrlyscysthrleulys
290295300
serphethrvalglulysglyiletyrglnthrserasnpheargval
305310315320
glnprothrgluserilevalargpheproasnilethrasnleucys
325330335
propheglygluvalpheasnalathrargphealaservaltyrala
340345350
trpasnarglysargileserasncysvalalaasptyrservalleu
355360365
tyrasnseralaserpheserthrphelyscystyrglyvalserpro
370375380
thrlysleuasnaspleucysphethrasnvaltyralaaspserphe
385390395400
valileargglyaspgluvalargglnilealaproglyglnthrgly
405410415
lysilealaasptyrasntyrlysleuproaspaspphethrglycys
420425430
valilealatrpasnserasnasnleuaspserlysvalglyglyasn
435440445
tyrasntyrleutyrargleuphearglysserasnleulysprophe
450455460
gluargaspileserthrgluiletyrglnalaglyserthrprocys
465470475480
asnglyvalgluglypheasncystyrpheproleuglnsertyrgly
485490495
pheglnprothrasnglyvalglytyrglnprotyrargvalvalval
500505510
leuserphegluleuleuhisalaproalathrvalcysglyprolys
515520525
lysserthrasnleuvallysasnlyscysvalasnpheasnpheasn
530535540
glyleuthrglythrglyvalleuthrgluserasnlyslyspheleu
545550555560
propheglnglnpheglyargaspilealaaspthrthraspalaval
565570575
argaspproglnthrleugluileleuaspilethrprocysserphe
580585590
glyglyvalservalilethrproglythrasnthrserasnglnval
595600605
alavalleutyrglnaspvalasncysthrgluvalprovalalaile
610615620
hisalaaspglnleuthrprothrtrpargvaltyrserthrglyser
625630635640
asnvalpheglnthrargalaglycysleuileglyalagluhisval
645650655
asnasnsertyrglucysaspileproileglyalaglyilecysala
660665670
sertyrglnthrglnthrasnserproglyglyserglyservalala
675680685
serglnserileilealatyrthrmetserleuglyalagluasnser
690695700
valalatyrserasnasnserilealaileprothrasnphethrile
705710715720
servalthrthrgluileleuprovalsermetthrlysthrserval
725730735
aspcysthrmettyrilecysglyaspserthrglucysserasnleu
740745750
leuleuglntyrglyserphecysthrglnleuasnargalaleuthr
755760765
glyilealavalgluglnasplysasnthrglngluvalphealagln
770775780
vallysglnproaspproserlysproseralaasnserpheileglu
785790795800
aspleuleupheasnlysvalthrleualaaspalaglypheilelys
805810815
glntyrglyaspcysleuglyaspilealaalaargaspleuilecys
820825830
alaglnlyspheasnglyleuthrvalleuproproleuleuthrasp
835840845
glumetilealaglntyrthrseralaleuleualaglythrilethr
850855860
serglytrpthrpheglyalaglyalaalaleuglnilepropheala
865870875880
metglnmetalatyrargpheasnglyileglyvalthrglnasnval
885890895
leutyrgluasnglnlysleuilealaasnglnpheasnseralaile
900905910
glylysileglnaspserleuserserthralaseralaleuglylys
915920925
leuglnaspvalvalasnglnasnalaglnalaleuasnthrleuval
930935940
lysglnleuserserasnpheglyalaileserservalleuasnasp
945950955960
ileleuserargleuaspproproglualagluvalglnileasparg
965970975
leuilethrglyargleuglnserleuglnthrtyrvalthrglngln
980985990
leuileargalaalagluileargalaseralaasnleualaalathr
99510001005
lysmetserglucysvalleuglyglnserlysargvalaspphe
101010151020
cysglylysglytyrhisleumetserpheproglnseralapro
102510301035
hisglyvalvalpheleuhisvalthrtyrvalproalaglnglu
104010451050
lysasnphethrthralaproalailecyshisaspglylysala
105510601065
hispheproarggluglyvalphevalserasnglythrhistrp
107010751080
phevalthrglnargasnphetyrgluproglnileilethrthr
108510901095
aspasnthrphevalserglyasncysaspvalvalileglyile
110011051110
valasnasnthrvaltyraspproleuglnprogluleuaspser
111511201125
phelysglugluleuasplystyrphelysasnhisthrserpro
113011351140
aspvalaspleuglyaspileserglyileasnalaservalval
114511501155
asnileglnlysgluileaspargleuasngluvalalalysasn
116011651170
leuasngluserleuileaspleuglngluleuglylystyrglu
117511801185
glntyrilelystrppro
1190
<210>33
<211>1237
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<213>人工序列
<220>
<223>重组sars-cov-2s蛋白
<400>33
metphevalpheleuvalleuleuproleuvalserserglncysval
151015
asnleuthrthrargthrglnleuproproalatyrthrasnserphe
202530
thrargglyvaltyrtyrproasplysvalpheargserservalleu
354045
hisserthrglnaspleupheleuprophepheserasnvalthrtrp
505560
phehisalailehisvalserglythrasnglythrlysargpheasp
65707580
asnprovalleupropheasnaspglyvaltyrphealaserthrglu
859095
lysserasnileileargglytrpilepheglythrthrleuaspser
100105110
lysthrglnserleuleuilevalasnasnalathrasnvalvalile
115120125
lysvalcysglupheglnphecysasnasppropheleuglyvaltyr
130135140
tyrhislysasnasnlyssertrpmetgluserglupheargvaltyr
145150155160
serseralaasnasncysthrpheglutyrvalserglnpropheleu
165170175
metaspleugluglylysglnglyasnphelysasnleuarggluphe
180185190
valphelysasnileaspglytyrphelysiletyrserlyshisthr
195200205
proileasnleuvalargaspleuproglnglypheseralaleuglu
210215220
proleuvalaspleuproileglyileasnilethrargpheglnthr
225230235240
leuleualaleuhisargsertyrleuthrproglyaspserserser
245250255
glytrpthralaglyalaalaalatyrtyrvalglytyrleuglnpro
260265270
argthrpheleuleulystyrasngluasnglythrilethraspala
275280285
valaspcysalaleuaspproleusergluthrlyscysthrleulys
290295300
serphethrvalglulysglyiletyrglnthrserasnpheargval
305310315320
glnprothrgluserilevalargpheproasnilethrasnleucys
325330335
propheglygluvalpheasnalathrargphealaservaltyrala
340345350
trpasnarglysargileserasncysvalalaasptyrservalleu
355360365
tyrasnseralaserpheserthrphelyscystyrglyvalserpro
370375380
thrlysleuasnaspleucysphethrasnvaltyralaaspserphe
385390395400
valileargglyaspgluvalargglnilealaproglyglnthrgly
405410415
lysilealaasptyrasntyrlysleuproaspaspphethrglycys
420425430
valilealatrpasnserasnasnleuaspserlysvalglyglyasn
435440445
tyrasntyrleutyrargleuphearglysserasnleulysprophe
450455460
gluargaspileserthrgluiletyrglnalaglyserthrprocys
465470475480
asnglyvalgluglypheasncystyrpheproleuglnsertyrgly
485490495
pheglnprothrasnglyvalglytyrglnprotyrargvalvalval
500505510
leuserphegluleuleuhisalaproalathrvalcysglyprolys
515520525
lysserthrasnleuvallysasnlyscysvalasnpheasnpheasn
530535540
glyleuthrglythrglyvalleuthrgluserasnlyslyspheleu
545550555560
propheglnglnpheglyargaspilealaaspthrthraspalaval
565570575
argaspproglnthrleugluileleuaspilethrprocysserphe
580585590
glyglyvalservalilethrproglythrasnthrserasnglnval
595600605
alavalleutyrglnaspvalasncysthrgluvalprovalalaile
610615620
hisalaaspglnleuthrprothrtrpargvaltyrserthrglyser
625630635640
asnvalpheglnthrargalaglycysleuileglyalagluhisval
645650655
asnasnsertyrglucysaspileproileglyalaglyilecysala
660665670
sertyrglnthrglnthrasnserproglyglyserglyservalala
675680685
serglnserileilealatyrthrmetserleuglyalagluasnser
690695700
valalatyrserasnasnserilealaileprothrasnphethrile
705710715720
servalthrthrgluileleuprovalsermetthrlysthrserval
725730735
aspcysthrmettyrilecysglyaspserthrglucysserasnleu
740745750
leuleuglntyrglyserphecysthrglnleuasnargalaleuthr
755760765
glyilealavalgluglnasplysasnthrglngluvalphealagln
770775780
vallysglniletyrlysthrproproilelysasppheglyglyphe
785790795800
asnpheserglnileleuproaspproserlysproseralaasnser
805810815
pheilegluaspleuleupheasnlysvalthrleualaaspalagly
820825830
pheilelysglntyrglyaspcysleuglyaspilealaalaargasp
835840845
leuilecysalaglnlyspheasnglyleuthrvalleuproproleu
850855860
leuthraspglumetilealaglntyrthrseralaleuleualagly
865870875880
thrilethrserglytrpthrpheglyalaglyalaalaleuglnile
885890895
prophealametglnmetalatyrargpheasnglyileglyvalthr
900905910
glnasnvalleutyrgluasnglnlysleuilealaasnglnpheasn
915920925
seralaileglylysileglnaspserleuserserthralaserala
930935940
leuglylysleuglnaspvalvalasnglnasnalaglnalaleuasn
945950955960
thrleuvallysglnleuserserasnpheglyalaileserserval
965970975
leuasnaspileleuserargleuasplysproglualagluvalgln
980985990
ileaspargleuilethrglyargleuglnserleuglnthrtyrval
99510001005
thrglnglnleuileargalaalagluileargalaseralaasn
101010151020
leualaalathrlysmetserglucysvalleuglyglnserlys
102510301035
argvalaspphecysglylysglytyrhisleumetserphepro
104010451050
glnseralaprohisglyvalvalpheleuhisvalthrtyrval
105510601065
proalaglnglulysasnphethrthralaproalailecyshis
107010751080
aspglylysalahispheproarggluglyvalphevalserasn
108510901095
glythrhistrpphevalthrglnargasnphetyrgluprogln
110011051110
ileilethrthraspasnthrphevalserglyasncysaspval
111511201125
valileglyilevalasnasnthrvaltyraspproleuglnpro
113011351140
gluleuaspserphelysglugluleuasplystyrphelysasn
114511501155
histhrserproaspvalaspleuglyaspileserglyileasn
116011651170
alaservalvalasnileglnlysgluileaspargleuasnglu
117511801185
valalalysasnleuasngluserleuileaspleuglngluleu
119011951200
glylystyrgluglntyrilelystrpprotrptyriletrpleu
120512101215
glypheilealaglyleuilealailevalmetvalthrilemet
122012251230
leucyscysmet
1235
<210>34
<211>1237
<212>prt
<213>人工序列
<220>
<223>重组sars-cov-2s蛋白
<400>34
metphevalpheleuvalleuleuproleuvalserserglncysval
151015
asnleuthrthrargthrglnleuproproalatyrthrasnserphe
202530
thrargglyvaltyrtyrproasplysvalpheargserservalleu
354045
hisserthrglnaspleupheleuprophepheserasnvalthrtrp
505560
phehisalailehisvalserglythrasnglythrlysargpheasp
65707580
asnprovalleupropheasnaspglyvaltyrphealaserthrglu
859095
lysserasnileileargglytrpilepheglythrthrleuaspser
100105110
lysthrglnserleuleuilevalasnasnalathrasnvalvalile
115120125
lysvalcysglupheglnphecysasnasppropheleuglyvaltyr
130135140
tyrhislysasnasnlyssertrpmetgluserglupheargvaltyr
145150155160
serseralaasnasncysthrpheglutyrvalserglnpropheleu
165170175
metaspleugluglylysglnglyasnphelysasnleuarggluphe
180185190
valphelysasnileaspglytyrphelysiletyrserlyshisthr
195200205
proileasnleuvalargaspleuproglnglypheseralaleuglu
210215220
proleuvalaspleuproileglyileasnilethrargpheglnthr
225230235240
leuleualaleuhisargsertyrleuthrproglyaspserserser
245250255
glytrpthralaglyalaalaalatyrtyrvalglytyrleuglnpro
260265270
argthrpheleuleulystyrasngluasnglythrilethraspala
275280285
valaspcysalaleuaspproleusergluthrlyscysthrleulys
290295300
serphethrvalglulysglyiletyrglnthrserasnpheargval
305310315320
glnprothrgluserilevalargpheproasnilethrasnleucys
325330335
propheglygluvalpheasnalathrargphealaservaltyrala
340345350
trpasnarglysargileserasncysvalalaasptyrservalleu
355360365
tyrasnseralaserpheserthrphelyscystyrglyvalserpro
370375380
thrlysleuasnaspleucysphethrasnvaltyralaaspserphe
385390395400
valileargglyaspgluvalargglnilealaproglyglnthrgly
405410415
lysilealaasptyrasntyrlysleuproaspaspphethrglycys
420425430
valilealatrpasnserasnasnleuaspserlysvalglyglyasn
435440445
tyrasntyrleutyrargleuphearglysserasnleulysprophe
450455460
gluargaspileserthrgluiletyrglnalaglyserthrprocys
465470475480
asnglyvalgluglypheasncystyrpheproleuglnsertyrgly
485490495
pheglnprothrasnglyvalglytyrglnprotyrargvalvalval
500505510
leuserphegluleuleuhisalaproalathrvalcysglyprolys
515520525
lysserthrasnleuvallysasnlyscysvalasnpheasnpheasn
530535540
glyleuthrglythrglyvalleuthrgluserasnlyslyspheleu
545550555560
propheglnglnpheglyargaspilealaaspthrthraspalaval
565570575
argaspproglnthrleugluileleuaspilethrprocysserphe
580585590
glyglyvalservalilethrproglythrasnthrserasnglnval
595600605
alavalleutyrglnaspvalasncysthrgluvalprovalalaile
610615620
hisalaaspglnleuthrprothrtrpargvaltyrserthrglyser
625630635640
asnvalpheglnthrargalaglycysleuileglyalagluhisval
645650655
asnasnsertyrglucysaspileproileglyalaglyilecysala
660665670
sertyrglnthrglnthrasnserproglyglyserglyservalala
675680685
serglnserileilealatyrthrmetserleuglyalagluasnser
690695700
valalatyrserasnasnserilealaileprothrasnphethrile
705710715720
servalthrthrgluileleuprovalsermetthrlysthrserval
725730735
aspcysthrmettyrilecysglyaspserthrglucysserasnleu
740745750
leuleuglntyrglyserphecysthrglnleuasnargalaleuthr
755760765
glyilealavalgluglnasplysasnthrglngluvalphealagln
770775780
vallysglniletyrlysthrproproilelysasppheglyglyphe
785790795800
asnpheserglnileleuproaspproserlysproseralaasnser
805810815
pheilegluaspleuleupheasnlysvalthrleualaaspalagly
820825830
pheilelysglntyrglyaspcysleuglyaspilealaalaargasp
835840845
leuilecysalaglnlyspheasnglyleuthrvalleuproproleu
850855860
leuthraspglumetilealaglntyrthrseralaleuleualagly
865870875880
thrilethrserglytrpthrpheglyalaglyalaalaleuglnile
885890895
prophealametglnmetalatyrargpheasnglyileglyvalthr
900905910
glnasnvalleutyrgluasnglnlysleuilealaasnglnpheasn
915920925
seralaileglylysileglnaspserleuserserthralaserala
930935940
leuglylysleuglnaspvalvalasnglnasnalaglnalaleuasn
945950955960
thrleuvallysglnleuserserasnpheglyalaileserserval
965970975
leuasnaspileleuserargleuaspprovalglualagluvalgln
980985990
ileaspargleuilethrglyargleuglnserleuglnthrtyrval
99510001005
thrglnglnleuileargalaalagluileargalaseralaasn
101010151020
leualaalathrlysmetserglucysvalleuglyglnserlys
102510301035
argvalaspphecysglylysglytyrhisleumetserphepro
104010451050
glnseralaprohisglyvalvalpheleuhisvalthrtyrval
105510601065
proalaglnglulysasnphethrthralaproalailecyshis
107010751080
aspglylysalahispheproarggluglyvalphevalserasn
108510901095
glythrhistrpphevalthrglnargasnphetyrgluprogln
110011051110
ileilethrthraspasnthrphevalserglyasncysaspval
111511201125
valileglyilevalasnasnthrvaltyraspproleuglnpro
113011351140
gluleuaspserphelysglugluleuasplystyrphelysasn
114511501155
histhrserproaspvalaspleuglyaspileserglyileasn
116011651170
alaservalvalasnileglnlysgluileaspargleuasnglu
117511801185
valalalysasnleuasngluserleuileaspleuglngluleu
119011951200
glylystyrgluglntyrilelystrpprotrptyriletrpleu
120512101215
glypheilealaglyleuilealailevalmetvalthrilemet
122012251230
leucyscysmet
1235
<210>35
<211>1237
<212>prt
<213>人工序列
<220>
<223>重组sars-cov-2s蛋白
<400>35
metphevalpheleuvalleuleuproleuvalserserglncysval
151015
asnleuthrthrargthrglnleuproproalatyrthrasnserphe
202530
thrargglyvaltyrtyrproasplysvalpheargserservalleu
354045
hisserthrglnaspleupheleuprophepheserasnvalthrtrp
505560
phehisalailehisvalserglythrasnglythrlysargpheasp
65707580
asnprovalleupropheasnaspglyvaltyrphealaserthrglu
859095
lysserasnileileargglytrpilepheglythrthrleuaspser
100105110
lysthrglnserleuleuilevalasnasnalathrasnvalvalile
115120125
lysvalcysglupheglnphecysasnasppropheleuglyvaltyr
130135140
tyrhislysasnasnlyssertrpmetgluserglupheargvaltyr
145150155160
serseralaasnasncysthrpheglutyrvalserglnpropheleu
165170175
metaspleugluglylysglnglyasnphelysasnleuarggluphe
180185190
valphelysasnileaspglytyrphelysiletyrserlyshisthr
195200205
proileasnleuvalargaspleuproglnglypheseralaleuglu
210215220
proleuvalaspleuproileglyileasnilethrargpheglnthr
225230235240
leuleualaleuhisargsertyrleuthrproglyaspserserser
245250255
glytrpthralaglyalaalaalatyrtyrvalglytyrleuglnpro
260265270
argthrpheleuleulystyrasngluasnglythrilethraspala
275280285
valaspcysalaleuaspproleusergluthrlyscysthrleulys
290295300
serphethrvalglulysglyiletyrglnthrserasnpheargval
305310315320
glnprothrgluserilevalargpheproasnilethrasnleucys
325330335
propheglygluvalpheasnalathrargphealaservaltyrala
340345350
trpasnarglysargileserasncysvalalaasptyrservalleu
355360365
tyrasnseralaserpheserthrphelyscystyrglyvalserpro
370375380
thrlysleuasnaspleucysphethrasnvaltyralaaspserphe
385390395400
valileargglyaspgluvalargglnilealaproglyglnthrgly
405410415
lysilealaasptyrasntyrlysleuproaspaspphethrglycys
420425430
valilealatrpasnserasnasnleuaspserlysvalglyglyasn
435440445
tyrasntyrleutyrargleuphearglysserasnleulysprophe
450455460
gluargaspileserthrgluiletyrglnalaglyserthrprocys
465470475480
asnglyvalgluglypheasncystyrpheproleuglnsertyrgly
485490495
pheglnprothrasnglyvalglytyrglnprotyrargvalvalval
500505510
leuserphegluleuleuhisalaproalathrvalcysglyprolys
515520525
lysserthrasnleuvallysasnlyscysvalasnpheasnpheasn
530535540
glyleuthrglythrglyvalleuthrgluserasnlyslyspheleu
545550555560
propheglnglnpheglyargaspilealaaspthrthraspalaval
565570575
argaspproglnthrleugluileleuaspilethrprocysserphe
580585590
glyglyvalservalilethrproglythrasnthrserasnglnval
595600605
alavalleutyrglnaspvalasncysthrgluvalprovalalaile
610615620
hisalaaspglnleuthrprothrtrpargvaltyrserthrglyser
625630635640
asnvalpheglnthrargalaglycysleuileglyalagluhisval
645650655
asnasnsertyrglucysaspileproileglyalaglyilecysala
660665670
sertyrglnthrglnthrasnserproglyglyserglyservalala
675680685
serglnserileilealatyrthrmetserleuglyalagluasnser
690695700
valalatyrserasnasnserilealaileprothrasnphethrile
705710715720
servalthrthrgluileleuprovalsermetthrlysthrserval
725730735
aspcysthrmettyrilecysglyaspserthrglucysserasnleu
740745750
leuleuglntyrglyserphecysthrglnleuasnargalaleuthr
755760765
glyilealavalgluglnasplysasnthrglngluvalphealagln
770775780
vallysglniletyrlysthrproproilelysasppheglyglyphe
785790795800
asnpheserglnileleuproaspproserlysproseralaasnser
805810815
pheilegluaspleuleupheasnlysvalthrleualaaspalagly
820825830
pheilelysglntyrglyaspcysleuglyaspilealaalaargasp
835840845
leuilecysalaglnlyspheasnglyleuthrvalleuproproleu
850855860
leuthraspglumetilealaglntyrthrseralaleuleualagly
865870875880
thrilethrserglytrpthrpheglyalaglyalaalaleuglnile
885890895
prophealametglnmetalatyrargpheasnglyileglyvalthr
900905910
glnasnvalleutyrgluasnglnlysleuilealaasnglnpheasn
915920925
seralaileglylysileglnaspserleuserserthralaserala
930935940
leuglylysleuglnaspvalvalasnglnasnalaglnalaleuasn
945950955960
thrleuvallysglnleuserserasnpheglyalaileserserval
965970975
leuasnaspileleuserargleuaspproproglualagluvalgln
980985990
ileaspargleuilethrglyargleuglnserleuglnthrtyrval
99510001005
thrglnglnleuileargalaalagluileargalaseralaasn
101010151020
leualaalathrlysmetserglucysvalleuglyglnserlys
102510301035
argvalaspphecysglylysglytyrhisleumetserphepro
104010451050
glnseralaprohisglyvalvalpheleuhisvalthrtyrval
105510601065
proalaglnglulysasnphethrthralaproalailecyshis
107010751080
aspglylysalahispheproarggluglyvalphevalserasn
108510901095
glythrhistrpphevalthrglnargasnphetyrgluprogln
110011051110
ileilethrthraspasnthrphevalserglyasncysaspval
111511201125
valileglyilevalasnasnthrvaltyraspproleuglnpro
113011351140
gluleuaspserphelysglugluleuasplystyrphelysasn
114511501155
histhrserproaspvalaspleuglyaspileserglyileasn
116011651170
alaservalvalasnileglnlysgluileaspargleuasnglu
117511801185
valalalysasnleuasngluserleuileaspleuglngluleu
119011951200
glylystyrgluglntyrilelystrpprotrptyriletrpleu
120512101215
glypheilealaglyleuilealailevalmetvalthrilemet
122012251230
leucyscysmet
1235
<210>36
<211>1218
<212>prt
<213>人工序列
<220>
<223>重组sars-cov-2s蛋白
<400>36
metphevalpheleuvalleuleuproleuvalserserglncysval
151015
asnleuthrthrargthrglnleuproproalatyrthrasnserphe
202530
thrargglyvaltyrtyrproasplysvalpheargserservalleu
354045
hisserthrglnaspleupheleuprophepheserasnvalthrtrp
505560
phehisalailehisvalserglythrasnglythrlysargpheasp
65707580
asnprovalleupropheasnaspglyvaltyrphealaserthrglu
859095
lysserasnileileargglytrpilepheglythrthrleuaspser
100105110
lysthrglnserleuleuilevalasnasnalathrasnvalvalile
115120125
lysvalcysglupheglnphecysasnasppropheleuglyvaltyr
130135140
tyrhislysasnasnlyssertrpmetgluserglupheargvaltyr
145150155160
serseralaasnasncysthrpheglutyrvalserglnpropheleu
165170175
metaspleugluglylysglnglyasnphelysasnleuarggluphe
180185190
valphelysasnileaspglytyrphelysiletyrserlyshisthr
195200205
proileasnleuvalargaspleuproglnglypheseralaleuglu
210215220
proleuvalaspleuproileglyileasnilethrargpheglnthr
225230235240
leuleualaleuhisargsertyrleuthrproglyaspserserser
245250255
glytrpthralaglyalaalaalatyrtyrvalglytyrleuglnpro
260265270
argthrpheleuleulystyrasngluasnglythrilethraspala
275280285
valaspcysalaleuaspproleusergluthrlyscysthrleulys
290295300
serphethrvalglulysglyiletyrglnthrserasnpheargval
305310315320
glnprothrgluserilevalargpheproasnilethrasnleucys
325330335
propheglygluvalpheasnalathrargphealaservaltyrala
340345350
trpasnarglysargileserasncysvalalaasptyrservalleu
355360365
tyrasnseralaserpheserthrphelyscystyrglyvalserpro
370375380
thrlysleuasnaspleucysphethrasnvaltyralaaspserphe
385390395400
valileargglyaspgluvalargglnilealaproglyglnthrgly
405410415
lysilealaasptyrasntyrlysleuproaspaspphethrglycys
420425430
valilealatrpasnserasnasnleuaspserlysvalglyglyasn
435440445
tyrasntyrleutyrargleuphearglysserasnleulysprophe
450455460
gluargaspileserthrgluiletyrglnalaglyserthrprocys
465470475480
asnglyvalgluglypheasncystyrpheproleuglnsertyrgly
485490495
pheglnprothrasnglyvalglytyrglnprotyrargvalvalval
500505510
leuserphegluleuleuhisalaproalathrvalcysglyprolys
515520525
lysserthrasnleuvallysasnlyscysvalasnpheasnpheasn
530535540
glyleuthrglythrglyvalleuthrgluserasnlyslyspheleu
545550555560
propheglnglnpheglyargaspilealaaspthrthraspalaval
565570575
argaspproglnthrleugluileleuaspilethrprocysserphe
580585590
glyglyvalservalilethrproglythrasnthrserasnglnval
595600605
alavalleutyrglnaspvalasncysthrgluvalprovalalaile
610615620
hisalaaspglnleuthrprothrtrpargvaltyrserthrglyser
625630635640
asnvalpheglnthrargalaglycysleuileglyalagluhisval
645650655
asnasnsertyrglucysaspileproileglyalaglyilecysala
660665670
sertyrglnthrglnthrasnserproglyglyserglyservalala
675680685
serglnserileilealatyrthrmetserleuglyalagluasnser
690695700
valalatyrserasnasnserilealaileprothrasnphethrile
705710715720
servalthrthrgluileleuprovalsermetthrlysthrserval
725730735
aspcysthrmettyrilecysglyaspserthrglucysserasnleu
740745750
leuleuglntyrglyserphecysthrglnleuasnargalaleuthr
755760765
glyilealavalgluglnasplysasnthrglngluvalphealagln
770775780
vallysglnproaspproserlysproseralaasnserpheileglu
785790795800
aspleuleupheasnlysvalthrleualaaspalaglypheilelys
805810815
glntyrglyaspcysleuglyaspilealaalaargaspleuilecys
820825830
alaglnlyspheasnglyleuthrvalleuproproleuleuthrasp
835840845
glumetilealaglntyrthrseralaleuleualaglythrilethr
850855860
serglytrpthrpheglyalaglyalaalaleuglnilepropheala
865870875880
metglnmetalatyrargpheasnglyileglyvalthrglnasnval
885890895
leutyrgluasnglnlysleuilealaasnglnpheasnseralaile
900905910
glylysileglnaspserleuserserthralaseralaleuglylys
915920925
leuglnaspvalvalasnglnasnalaglnalaleuasnthrleuval
930935940
lysglnleuserserasnpheglyalaileserservalleuasnasp
945950955960
ileleuserargleuaspprovalglualagluvalglnileasparg
965970975
leuilethrglyargleuglnserleuglnthrtyrvalthrglngln
980985990
leuileargalaalagluileargalaseralaasnleualaalathr
99510001005
lysmetserglucysvalleuglyglnserlysargvalaspphe
101010151020
cysglylysglytyrhisleumetserpheproglnseralapro
102510301035
hisglyvalvalpheleuhisvalthrtyrvalproalaglnglu
104010451050
lysasnphethrthralaproalailecyshisaspglylysala
105510601065
hispheproarggluglyvalphevalserasnglythrhistrp
107010751080
phevalthrglnargasnphetyrgluproglnileilethrthr
108510901095
aspasnthrphevalserglyasncysaspvalvalileglyile
110011051110
valasnasnthrvaltyraspproleuglnprogluleuaspser
111511201125
phelysglugluleuasplystyrphelysasnhisthrserpro
113011351140
aspvalaspleuglyaspileserglyileasnalaservalval
114511501155
asnileglnlysgluileaspargleuasngluvalalalysasn
116011651170
leuasngluserleuileaspleuglngluleuglylystyrglu
117511801185
glntyrilelystrpprotrptyriletrpleuglypheileala
119011951200
glyleuilealailevalmetvalthrilemetleucyscysmet
120512101215
<210>37
<211>1218
<212>prt
<213>人工序列
<220>
<223>重组sars-cov-2s蛋白
<400>37
metphevalpheleuvalleuleuproleuvalserserglncysval
151015
asnleuthrthrargthrglnleuproproalatyrthrasnserphe
202530
thrargglyvaltyrtyrproasplysvalpheargserservalleu
354045
hisserthrglnaspleupheleuprophepheserasnvalthrtrp
505560
phehisalailehisvalserglythrasnglythrlysargpheasp
65707580
asnprovalleupropheasnaspglyvaltyrphealaserthrglu
859095
lysserasnileileargglytrpilepheglythrthrleuaspser
100105110
lysthrglnserleuleuilevalasnasnalathrasnvalvalile
115120125
lysvalcysglupheglnphecysasnasppropheleuglyvaltyr
130135140
tyrhislysasnasnlyssertrpmetgluserglupheargvaltyr
145150155160
serseralaasnasncysthrpheglutyrvalserglnpropheleu
165170175
metaspleugluglylysglnglyasnphelysasnleuarggluphe
180185190
valphelysasnileaspglytyrphelysiletyrserlyshisthr
195200205
proileasnleuvalargaspleuproglnglypheseralaleuglu
210215220
proleuvalaspleuproileglyileasnilethrargpheglnthr
225230235240
leuleualaleuhisargsertyrleuthrproglyaspserserser
245250255
glytrpthralaglyalaalaalatyrtyrvalglytyrleuglnpro
260265270
argthrpheleuleulystyrasngluasnglythrilethraspala
275280285
valaspcysalaleuaspproleusergluthrlyscysthrleulys
290295300
serphethrvalglulysglyiletyrglnthrserasnpheargval
305310315320
glnprothrgluserilevalargpheproasnilethrasnleucys
325330335
propheglygluvalpheasnalathrargphealaservaltyrala
340345350
trpasnarglysargileserasncysvalalaasptyrservalleu
355360365
tyrasnseralaserpheserthrphelyscystyrglyvalserpro
370375380
thrlysleuasnaspleucysphethrasnvaltyralaaspserphe
385390395400
valileargglyaspgluvalargglnilealaproglyglnthrgly
405410415
lysilealaasptyrasntyrlysleuproaspaspphethrglycys
420425430
valilealatrpasnserasnasnleuaspserlysvalglyglyasn
435440445
tyrasntyrleutyrargleuphearglysserasnleulysprophe
450455460
gluargaspileserthrgluiletyrglnalaglyserthrprocys
465470475480
asnglyvalgluglypheasncystyrpheproleuglnsertyrgly
485490495
pheglnprothrasnglyvalglytyrglnprotyrargvalvalval
500505510
leuserphegluleuleuhisalaproalathrvalcysglyprolys
515520525
lysserthrasnleuvallysasnlyscysvalasnpheasnpheasn
530535540
glyleuthrglythrglyvalleuthrgluserasnlyslyspheleu
545550555560
propheglnglnpheglyargaspilealaaspthrthraspalaval
565570575
argaspproglnthrleugluileleuaspilethrprocysserphe
580585590
glyglyvalservalilethrproglythrasnthrserasnglnval
595600605
alavalleutyrglnaspvalasncysthrgluvalprovalalaile
610615620
hisalaaspglnleuthrprothrtrpargvaltyrserthrglyser
625630635640
asnvalpheglnthrargalaglycysleuileglyalagluhisval
645650655
asnasnsertyrglucysaspileproileglyalaglyilecysala
660665670
sertyrglnthrglnthrasnserproglyglyserglyservalala
675680685
serglnserileilealatyrthrmetserleuglyalagluasnser
690695700
valalatyrserasnasnserilealaileprothrasnphethrile
705710715720
servalthrthrgluileleuprovalsermetthrlysthrserval
725730735
aspcysthrmettyrilecysglyaspserthrglucysserasnleu
740745750
leuleuglntyrglyserphecysthrglnleuasnargalaleuthr
755760765
glyilealavalgluglnasplysasnthrglngluvalphealagln
770775780
vallysglnproaspproserlysproseralaasnserpheileglu
785790795800
aspleuleupheasnlysvalthrleualaaspalaglypheilelys
805810815
glntyrglyaspcysleuglyaspilealaalaargaspleuilecys
820825830
alaglnlyspheasnglyleuthrvalleuproproleuleuthrasp
835840845
glumetilealaglntyrthrseralaleuleualaglythrilethr
850855860
serglytrpthrpheglyalaglyalaalaleuglnilepropheala
865870875880
metglnmetalatyrargpheasnglyileglyvalthrglnasnval
885890895
leutyrgluasnglnlysleuilealaasnglnpheasnseralaile
900905910
glylysileglnaspserleuserserthralaseralaleuglylys
915920925
leuglnaspvalvalasnglnasnalaglnalaleuasnthrleuval
930935940
lysglnleuserserasnpheglyalaileserservalleuasnasp
945950955960
ileleuserargleuasplysproglualagluvalglnileasparg
965970975
leuilethrglyargleuglnserleuglnthrtyrvalthrglngln
980985990
leuileargalaalagluileargalaseralaasnleualaalathr
99510001005
lysmetserglucysvalleuglyglnserlysargvalaspphe
101010151020
cysglylysglytyrhisleumetserpheproglnseralapro
102510301035
hisglyvalvalpheleuhisvalthrtyrvalproalaglnglu
104010451050
lysasnphethrthralaproalailecyshisaspglylysala
105510601065
hispheproarggluglyvalphevalserasnglythrhistrp
107010751080
phevalthrglnargasnphetyrgluproglnileilethrthr
108510901095
aspasnthrphevalserglyasncysaspvalvalileglyile
110011051110
valasnasnthrvaltyraspproleuglnprogluleuaspser
111511201125
phelysglugluleuasplystyrphelysasnhisthrserpro
113011351140
aspvalaspleuglyaspileserglyileasnalaservalval
114511501155
asnileglnlysgluileaspargleuasngluvalalalysasn
116011651170
leuasngluserleuileaspleuglngluleuglylystyrglu
117511801185
glntyrilelystrpprotrptyriletrpleuglypheileala
119011951200
glyleuilealailevalmetvalthrilemetleucyscysmet
120512101215
<210>38
<211>1218
<212>prt
<213>人工序列
<220>
<223>重组sars-cov-2s蛋白
<400>38
metphevalpheleuvalleuleuproleuvalserserglncysval
151015
asnleuthrthrargthrglnleuproproalatyrthrasnserphe
202530
thrargglyvaltyrtyrproasplysvalpheargserservalleu
354045
hisserthrglnaspleupheleuprophepheserasnvalthrtrp
505560
phehisalailehisvalserglythrasnglythrlysargpheasp
65707580
asnprovalleupropheasnaspglyvaltyrphealaserthrglu
859095
lysserasnileileargglytrpilepheglythrthrleuaspser
100105110
lysthrglnserleuleuilevalasnasnalathrasnvalvalile
115120125
lysvalcysglupheglnphecysasnasppropheleuglyvaltyr
130135140
tyrhislysasnasnlyssertrpmetgluserglupheargvaltyr
145150155160
serseralaasnasncysthrpheglutyrvalserglnpropheleu
165170175
metaspleugluglylysglnglyasnphelysasnleuarggluphe
180185190
valphelysasnileaspglytyrphelysiletyrserlyshisthr
195200205
proileasnleuvalargaspleuproglnglypheseralaleuglu
210215220
proleuvalaspleuproileglyileasnilethrargpheglnthr
225230235240
leuleualaleuhisargsertyrleuthrproglyaspserserser
245250255
glytrpthralaglyalaalaalatyrtyrvalglytyrleuglnpro
260265270
argthrpheleuleulystyrasngluasnglythrilethraspala
275280285
valaspcysalaleuaspproleusergluthrlyscysthrleulys
290295300
serphethrvalglulysglyiletyrglnthrserasnpheargval
305310315320
glnprothrgluserilevalargpheproasnilethrasnleucys
325330335
propheglygluvalpheasnalathrargphealaservaltyrala
340345350
trpasnarglysargileserasncysvalalaasptyrservalleu
355360365
tyrasnseralaserpheserthrphelyscystyrglyvalserpro
370375380
thrlysleuasnaspleucysphethrasnvaltyralaaspserphe
385390395400
valileargglyaspgluvalargglnilealaproglyglnthrgly
405410415
lysilealaasptyrasntyrlysleuproaspaspphethrglycys
420425430
valilealatrpasnserasnasnleuaspserlysvalglyglyasn
435440445
tyrasntyrleutyrargleuphearglysserasnleulysprophe
450455460
gluargaspileserthrgluiletyrglnalaglyserthrprocys
465470475480
asnglyvalgluglypheasncystyrpheproleuglnsertyrgly
485490495
pheglnprothrasnglyvalglytyrglnprotyrargvalvalval
500505510
leuserphegluleuleuhisalaproalathrvalcysglyprolys
515520525
lysserthrasnleuvallysasnlyscysvalasnpheasnpheasn
530535540
glyleuthrglythrglyvalleuthrgluserasnlyslyspheleu
545550555560
propheglnglnpheglyargaspilealaaspthrthraspalaval
565570575
argaspproglnthrleugluileleuaspilethrprocysserphe
580585590
glyglyvalservalilethrproglythrasnthrserasnglnval
595600605
alavalleutyrglnaspvalasncysthrgluvalprovalalaile
610615620
hisalaaspglnleuthrprothrtrpargvaltyrserthrglyser
625630635640
asnvalpheglnthrargalaglycysleuileglyalagluhisval
645650655
asnasnsertyrglucysaspileproileglyalaglyilecysala
660665670
sertyrglnthrglnthrasnserproglyglyserglyservalala
675680685
serglnserileilealatyrthrmetserleuglyalagluasnser
690695700
valalatyrserasnasnserilealaileprothrasnphethrile
705710715720
servalthrthrgluileleuprovalsermetthrlysthrserval
725730735
aspcysthrmettyrilecysglyaspserthrglucysserasnleu
740745750
leuleuglntyrglyserphecysthrglnleuasnargalaleuthr
755760765
glyilealavalgluglnasplysasnthrglngluvalphealagln
770775780
vallysglnproaspproserlysproseralaasnserpheileglu
785790795800
aspleuleupheasnlysvalthrleualaaspalaglypheilelys
805810815
glntyrglyaspcysleuglyaspilealaalaargaspleuilecys
820825830
alaglnlyspheasnglyleuthrvalleuproproleuleuthrasp
835840845
glumetilealaglntyrthrseralaleuleualaglythrilethr
850855860
serglytrpthrpheglyalaglyalaalaleuglnilepropheala
865870875880
metglnmetalatyrargpheasnglyileglyvalthrglnasnval
885890895
leutyrgluasnglnlysleuilealaasnglnpheasnseralaile
900905910
glylysileglnaspserleuserserthralaseralaleuglylys
915920925
leuglnaspvalvalasnglnasnalaglnalaleuasnthrleuval
930935940
lysglnleuserserasnpheglyalaileserservalleuasnasp
945950955960
ileleuserargleuaspproproglualagluvalglnileasparg
965970975
leuilethrglyargleuglnserleuglnthrtyrvalthrglngln
980985990
leuileargalaalagluileargalaseralaasnleualaalathr
99510001005
lysmetserglucysvalleuglyglnserlysargvalaspphe
101010151020
cysglylysglytyrhisleumetserpheproglnseralapro
102510301035
hisglyvalvalpheleuhisvalthrtyrvalproalaglnglu
104010451050
lysasnphethrthralaproalailecyshisaspglylysala
105510601065
hispheproarggluglyvalphevalserasnglythrhistrp
107010751080
phevalthrglnargasnphetyrgluproglnileilethrthr
108510901095
aspasnthrphevalserglyasncysaspvalvalileglyile
110011051110
valasnasnthrvaltyraspproleuglnprogluleuaspser
111511201125
phelysglugluleuasplystyrphelysasnhisthrserpro
113011351140
aspvalaspleuglyaspileserglyileasnalaservalval
114511501155
asnileglnlysgluileaspargleuasngluvalalalysasn
116011651170
leuasngluserleuileaspleuglngluleuglylystyrglu
117511801185
glntyrilelystrpprotrptyriletrpleuglypheileala
119011951200
glyleuilealailevalmetvalthrilemetleucyscysmet
120512101215
<210>39
<211>19
<212>prt
<213>人工序列
<220>
<223>igvh信号序列
<400>39
metasptrpiletrpargileleupheleuvalglyalaalathrgly
151015
alahisser
<210>40
<211>27
<212>prt
<213>人工序列
<220>
<223>t4噬菌体fibritin三聚体基序
<400>40
glytyrileproglualaproargaspglyglnalatyrvalarglys
151015
aspglyglutrpvalleuleuserthrpheleu
2025
<210>41
<211>62
<212>dna
<213>人工序列
<220>
<223>5'utr序列
<400>41
gtcccgcagtcggcgtccagcggctctgcttgttcgtgtgtgtgtcgttgcaggccttat60
tc62
<210>42
<211>24
<212>dna
<213>人工序列
<220>
<223>kozak序列
<400>42
agatctaccggtggtaccgccacc24
<210>43
<211>89
<212>dna
<213>人工序列
<220>
<223>3'utr序列
<400>43
agccaacaccctgtctaaaaaacataaatttctttaatcattttgcctcttttctctgtg60
cttcaattaataaaaaatggaaagaacct89
<210>44
<211>4024
<212>rna
<213>人工序列
<220>
<223>编码重组s蛋白的mrna
<400>44
gucccgcagucggcguccagcggcucugcuuguucgugugugugucguugcaggccuuau60
ucgccaccauggacuggaucuggagaauccuguuccuggugggggcugccacuggcgccc120
auagccagugcgugaaucugaccaccagaacccagcugccuccugccuacaccaauagcu180
ucaccagaggaguuuauuaucccgauaagguguucagaaguaguguauuacauaguaccc240
aggaccuguuccuaccuuucuucaguaacgugaccugguuccacgccauccacgugagcg300
gcaccaauggcaccaagagauucgacaauccugugcugccuuucaaugacggcguguacu360
ucgccagcaccgagaagagcaauaucaucagaggcuggaucuucggcaccaccuuggauu420
ccaagacucagagccugcugauuguaaacaacgcuacaaauguggugaucaaggugugcg480
aguuccaguucugcaaugacccuuuccuggguguuuauuaucauaagaacaacaagagcu540
ggauggagagcgaguuccgcguauauucgucggcuaauaauugcaccuucgaguacguga600
gccagccuuuccugauggaccuggagggcaagcagggcaauuucaagaaucugagagagu660
ucguguucaagaauaucgacggcuacuucaagaucuacagcaagcacacacccauuaauc720
uggugagagaccugccucagggcuucagcgcccuggagccucugguggaccugccuaucg780
gcaucaauaucaccagauuccagacccugcuggcccugcacagaucauaucuuacaccag840
gcgauucgucaagcgguuggaccgcuggagcugcggcauauuacgugggcuaccugcagc900
cuagaaccuuccugcugaaguacaaugagaaugguacgauaaccgacgcaguugauugug960
cccuggacccucugagcgagaccaagugcacccugaagagcuucaccguggagaagggca1020
ucuaccagaccagcaauuucagagugcagccuaccgagagcaucgugagauucccuaaua1080
ucaccaaucugugcccuuucggcgagguguucaaugccaccagauucgccagcguguacg1140
cauggaaccgcaagcggauaagcaauugcguggccgacuacagcgugcuguacaauagcg1200
ccagcuucagcaccuucaaauguuaugguguuucgccaacaaagcugaaugaccugugcu1260
ucaccaauguguacgccgacagcuucgugaucagaggcgacgaggugagacagaucgcgc1320
cagggcagaccggcaagaucgccgacuacaauuacaagcugccugacgacuucaccggcu1380
gcgugaucgcguggaacucuaacaaccuggacucgaaaguuggaggcaauuacaauuacc1440
uguacagacuguucagaaagagcaaucugaagccuuucgagagagacaucagcaccgaga1500
ucuaccaggccggcagcacaccguguaauggcguggagggcuucaauugcuacuucccuc1560
ugcagagcuacggcuuccagccuaccaauggcgugggcuaccagccuuacagaguggugg1620
ugcugagcuucgagcugcugcacgcucccgcuaccgugugcggcccuaagaagagcacca1680
aucuggugaagaauaagugcgugaauuucaauuucaauggucuaacuggaacgggcgugc1740
ugaccgagagcaauaagaaguuucuucccuuucaacaauucggcagagacaucgccgaca1800
ccacagaugcuguaagagacccucagacccuggagauccuggacaucacuccguguagcu1860
ucggcggcgugagcgugaucacaccgggcaccaauaccagcaaucagguggccgugcugu1920
accaggacgugaauugcaccgaggugccuguggccauccacgccgaccagcugacuccca1980
cuuggaggguauauuccacgggaagcaauguguuccagaccagagccggcugccugaucg2040
gcgccgagcacgugaauaauagcuacgagugcgacaucccuaucggcgccggcaucugcg2100
ccagcuaccagacccagaccaauagcccuggcggcagcggcagcguggccagccagagca2160
ucaucgccuacaccaugagccugggcgccgagaauagcguggccuacagcaauaauagca2220
ucgccaucccuaccaauuucaccaucagcgugaccaccgaaauauuaccagucuccauga2280
ccaagaccagcguggacugcaccauguacaucugcggcgacagcaccgagugcagcaauc2340
ugcugcugcaguacggcagcuucugcacccagcugaauagagcccugaccggcaucgccg2400
uggagcaggacaagaauacccaggagguguucgcccaggugaagcagaucuacaagacuc2460
cgccgaucaaggacuucggcggcuucaauuucagccaaauacucccagauccaagcaagc2520
cuagcgccaacagcuucaucgaggaccugcuguucaauaaggugacccuggccgacgccg2580
gcuucaucaagcaguacggcgacugccuaggugauauugcggcaagagaccugaucugcg2640
cccagaaguuuaacgguuugacaguacuaccuccucugcugaccgacgagaugauagcac2700
aauauacgucggcauugcucgcuggcacgaucacaucgggcuggacuuucggcgccggag2760
cagcguugcaaaucccuuucgccaugcagauggccuacagauucaauggcaucggcguga2820
cccagaaugugcuguacgagaaucagaagcugaucgccaaucaguucaauagcgccaucg2880
gcaagauccaggacagccugagcagcaccgccagcgcccugggcaagcugcaggacgugg2940
ugaaucagaaugcccaggcccugaauacccuggugaagcagcugagcagcaauuucggcg3000
ccaucaguaguguacucaacgacauccugagcagacuggacccgccggaggccgaggugc3060
aaauugaucgucuuauuacuggcagacugcagagccugcagaccuacgugacccagcagc3120
ugaucagagccgccgagaucagagccagcgccaaucuggccgccaccaagaugagcgagu3180
gcgugcugggccagagcaagagaguggacuucugcggcaagggcuaccaccugaugagcu3240
ucccucagagcgcuccacauggcgugguguuccugcacgugaccuacgugccugcccagg3300
agaagaauuucaccaccgcacccgcaaucugccacgacggcaaggcccacuucccuagag3360
agggcguguucgugagcaauggcacccacugguucgugacccagagaaauuucuacgagc3420
cucagaucaucaccaccgacaauaccuucgugagcggcaauugcgacguggugaucggga3480
uagucaauaauacugucuacgacccucugcagccugagcuggacagcuucaaggaggagc3540
uggacaaguacuucaagaaucacaccagcccugacguggaccucggugauauuucgggaa3600
ucaaugccagcguggugaauauccagaaggaaauugaucggcucaacgaaguggccaaga3660
aucugaaugagagccugaucgaccugcaggagcugggcaaguacgagcaguacaucaagu3720
ggccuagcgccaucggcggcuacauccccgaggcccccagggacggccaggccuacguga3780
ggaaggacggcgagugggugcugcugagcaccuuccugugaagccaacacccugucuaaa3840
aaacauaaauuucuuuaaucauuuugccucuuuucucugugcuucaauuaauaaaaaaug3900
gaaagaaccuaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaa3960
aaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaa4020
aaaa4024
<210>45
<211>3967
<212>rna
<213>人工序列
<220>
<223>编码重组s蛋白的mrna
<400>45
gucccgcagucggcguccagcggcucugcuuguucgugugugugucguugcaggccuuau60
ucgccaccauggacuggaucuggagaauccuguuccuggugggggcugccacuggcgccc120
auagccagugcgugaaucugaccaccagaacccagcugccuccugccuacaccaauagcu180
ucaccagaggaguuuauuaucccgauaagguguucagaaguaguguauuacauaguaccc240
aggaccuguuccuaccuuucuucaguaacgugaccugguuccacgccauccacgugagcg300
gcaccaauggcaccaagagauucgacaauccugugcugccuuucaaugacggcguguacu360
ucgccagcaccgagaagagcaauaucaucagaggcuggaucuucggcaccaccuuggauu420
ccaagacucagagccugcugauuguaaacaacgcuacaaauguggugaucaaggugugcg480
aguuccaguucugcaaugacccuuuccuggguguuuauuaucauaagaacaacaagagcu540
ggauggagagcgaguuccgcguauauucgucggcuaauaauugcaccuucgaguacguga600
gccagccuuuccugauggaccuggagggcaagcagggcaauuucaagaaucugagagagu660
ucguguucaagaauaucgacggcuacuucaagaucuacagcaagcacacacccauuaauc720
uggugagagaccugccucagggcuucagcgcccuggagccucugguggaccugccuaucg780
gcaucaauaucaccagauuccagacccugcuggcccugcacagaucauaucuuacaccag840
gcgauucgucaagcgguuggaccgcuggagcugcggcauauuacgugggcuaccugcagc900
cuagaaccuuccugcugaaguacaaugagaaugguacgauaaccgacgcaguugauugug960
cccuggacccucugagcgagaccaagugcacccugaagagcuucaccguggagaagggca1020
ucuaccagaccagcaauuucagagugcagccuaccgagagcaucgugagauucccuaaua1080
ucaccaaucugugcccuuucggcgagguguucaaugccaccagauucgccagcguguacg1140
cauggaaccgcaagcggauaagcaauugcguggccgacuacagcgugcuguacaauagcg1200
ccagcuucagcaccuucaaauguuaugguguuucgccaacaaagcugaaugaccugugcu1260
ucaccaauguguacgccgacagcuucgugaucagaggcgacgaggugagacagaucgcgc1320
cagggcagaccggcaagaucgccgacuacaauuacaagcugccugacgacuucaccggcu1380
gcgugaucgcguggaacucuaacaaccuggacucgaaaguuggaggcaauuacaauuacc1440
uguacagacuguucagaaagagcaaucugaagccuuucgagagagacaucagcaccgaga1500
ucuaccaggccggcagcacaccguguaauggcguggagggcuucaauugcuacuucccuc1560
ugcagagcuacggcuuccagccuaccaauggcgugggcuaccagccuuacagaguggugg1620
ugcugagcuucgagcugcugcacgcucccgcuaccgugugcggcccuaagaagagcacca1680
aucuggugaagaauaagugcgugaauuucaauuucaauggucuaacuggaacgggcgugc1740
ugaccgagagcaauaagaaguuucuucccuuucaacaauucggcagagacaucgccgaca1800
ccacagaugcuguaagagacccucagacccuggagauccuggacaucacuccguguagcu1860
ucggcggcgugagcgugaucacaccgggcaccaauaccagcaaucagguggccgugcugu1920
accaggacgugaauugcaccgaggugccuguggccauccacgccgaccagcugacuccca1980
cuuggaggguauauuccacgggaagcaauguguuccagaccagagccggcugccugaucg2040
gcgccgagcacgugaauaauagcuacgagugcgacaucccuaucggcgccggcaucugcg2100
ccagcuaccagacccagaccaauagcccuggcggcagcggcagcguggccagccagagca2160
ucaucgccuacaccaugagccugggcgccgagaauagcguggccuacagcaauaauagca2220
ucgccaucccuaccaauuucaccaucagcgugaccaccgaaauauuaccagucuccauga2280
ccaagaccagcguggacugcaccauguacaucugcggcgacagcaccgagugcagcaauc2340
ugcugcugcaguacggcagcuucugcacccagcugaauagagcccugaccggcaucgccg2400
uggagcaggacaagaauacccaggagguguucgcccaggugaagcagccagauccaagca2460
agccuagcgccaacagcuucaucgaggaccugcuguucaauaaggugacccuggccgacg2520
ccggcuucaucaagcaguacggcgacugccuaggugauauugcggcaagagaccugaucu2580
gcgcccagaaguuuaacgguuugacaguacuaccuccucugcugaccgacgagaugauag2640
cacaauauacgucggcauugcucgcuggcacgaucacaucgggcuggacuuucggcgccg2700
gagcagcguugcaaaucccuuucgccaugcagauggccuacagauucaauggcaucggcg2760
ugacccagaaugugcuguacgagaaucagaagcugaucgccaaucaguucaauagcgcca2820
ucggcaagauccaggacagccugagcagcaccgccagcgcccugggcaagcugcaggacg2880
uggugaaucagaaugcccaggcccugaauacccuggugaagcagcugagcagcaauuucg2940
gcgccaucaguaguguacucaacgacauccugagcagacuggacccgccggaggccgagg3000
ugcaaauugaucgucuuauuacuggcagacugcagagccugcagaccuacgugacccagc3060
agcugaucagagccgccgagaucagagccagcgccaaucuggccgccaccaagaugagcg3120
agugcgugcugggccagagcaagagaguggacuucugcggcaagggcuaccaccugauga3180
gcuucccucagagcgcuccacauggcgugguguuccugcacgugaccuacgugccugccc3240
aggagaagaauuucaccaccgcacccgcaaucugccacgacggcaaggcccacuucccua3300
gagagggcguguucgugagcaauggcacccacugguucgugacccagagaaauuucuacg3360
agccucagaucaucaccaccgacaauaccuucgugagcggcaauugcgacguggugaucg3420
ggauagucaauaauacugucuacgacccucugcagccugagcuggacagcuucaaggagg3480
agcuggacaaguacuucaagaaucacaccagcccugacguggaccucggugauauuucgg3540
gaaucaaugccagcguggugaauauccagaaggaaauugaucggcucaacgaaguggcca3600
agaaucugaaugagagccugaucgaccugcaggagcugggcaaguacgagcaguacauca3660
aguggccuagcgccaucggcggcuacauccccgaggcccccagggacggccaggccuacg3720
ugaggaaggacggcgagugggugcugcugagcaccuuccugugaagccaacacccugucu3780
aaaaaacauaaauuucuuuaaucauuuugccucuuuucucugugcuucaauuaauaaaaa3840
auggaaagaaccuaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaa3900
aaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaa3960
aaaaaaa3967
<210>46
<211>4096
<212>rna
<213>人工序列
<220>
<223>编码重组s蛋白的mrna
<400>46
gucccgcagucggcguccagcggcucugcuuguucgugugugugucguugcaggccuuau60
ucgccaccauggacuggaucuggagaauccuguuccuggugggggcugccacuggcgccc120
auagccagugcgugaaucugaccaccagaacccagcugccuccugccuacaccaauagcu180
ucaccagaggaguuuauuaucccgauaagguguucagaaguaguguauuacauaguaccc240
aggaccuguuccuaccuuucuucaguaacgugaccugguuccacgccauccacgugagcg300
gcaccaauggcaccaagagauucgacaauccugugcugccuuucaaugacggcguguacu360
ucgccagcaccgagaagagcaauaucaucagaggcuggaucuucggcaccaccuuggauu420
ccaagacucagagccugcugauuguaaacaacgcuacaaauguggugaucaaggugugcg480
aguuccaguucugcaaugacccuuuccuggguguuuauuaucauaagaacaacaagagcu540
ggauggagagcgaguuccgcguauauucgucggcuaauaauugcaccuucgaguacguga600
gccagccuuuccugauggaccuggagggcaagcagggcaauuucaagaaucugagagagu660
ucguguucaagaauaucgacggcuacuucaagaucuacagcaagcacacacccauuaauc720
uggugagagaccugccucagggcuucagcgcccuggagccucugguggaccugccuaucg780
gcaucaauaucaccagauuccagacccugcuggcccugcacagaucauaucuuacaccag840
gcgauucgucaagcgguuggaccgcuggagcugcggcauauuacgugggcuaccugcagc900
cuagaaccuuccugcugaaguacaaugagaaugguacgauaaccgacgcaguugauugug960
cccuggacccucugagcgagaccaagugcacccugaagagcuucaccguggagaagggca1020
ucuaccagaccagcaauuucagagugcagccuaccgagagcaucgugagauucccuaaua1080
ucaccaaucugugcccuuucggcgagguguucaaugccaccagauucgccagcguguacg1140
cauggaaccgcaagcggauaagcaauugcguggccgacuacagcgugcuguacaauagcg1200
ccagcuucagcaccuucaaauguuaugguguuucgccaacaaagcugaaugaccugugcu1260
ucaccaauguguacgccgacagcuucgugaucagaggcgacgaggugagacagaucgcgc1320
cagggcagaccggcaagaucgccgacuacaauuacaagcugccugacgacuucaccggcu1380
gcgugaucgcguggaacucuaacaaccuggacucgaaaguuggaggcaauuacaauuacc1440
uguacagacuguucagaaagagcaaucugaagccuuucgagagagacaucagcaccgaga1500
ucuaccaggccggcagcacaccguguaauggcguggagggcuucaauugcuacuucccuc1560
ugcagagcuacggcuuccagccuaccaauggcgugggcuaccagccuuacagaguggugg1620
ugcugagcuucgagcugcugcacgcucccgcuaccgugugcggcccuaagaagagcacca1680
aucuggugaagaauaagugcgugaauuucaauuucaauggucuaacuggaacgggcgugc1740
ugaccgagagcaauaagaaguuucuucccuuucaacaauucggcagagacaucgccgaca1800
ccacagaugcuguaagagacccucagacccuggagauccuggacaucacuccguguagcu1860
ucggcggcgugagcgugaucacaccgggcaccaauaccagcaaucagguggccgugcugu1920
accaggacgugaauugcaccgaggugccuguggccauccacgccgaccagcugacuccca1980
cuuggaggguauauuccacgggaagcaauguguuccagaccagagccggcugccugaucg2040
gcgccgagcacgugaauaauagcuacgagugcgacaucccuaucggcgccggcaucugcg2100
ccagcuaccagacccagaccaauagcccuggcggcagcggcagcguggccagccagagca2160
ucaucgccuacaccaugagccugggcgccgagaauagcguggccuacagcaauaauagca2220
ucgccaucccuaccaauuucaccaucagcgugaccaccgaaauauuaccagucuccauga2280
ccaagaccagcguggacugcaccauguacaucugcggcgacagcaccgagugcagcaauc2340
ugcugcugcaguacggcagcuucugcacccagcugaauagagcccugaccggcaucgccg2400
uggagcaggacaagaauacccaggagguguucgcccaggugaagcagaucuacaagacuc2460
cgccgaucaaggacuucggcggcuucaauuucagccaaauacucccagauccaagcaagc2520
cuagcgccaacagcuucaucgaggaccugcuguucaauaaggugacccuggccgacgccg2580
gcuucaucaagcaguacggcgacugccuaggugauauugcggcaagagaccugaucugcg2640
cccagaaguuuaacgguuugacaguacuaccuccucugcugaccgacgagaugauagcac2700
aauauacgucggcauugcucgcuggcacgaucacaucgggcuggacuuucggcgccggag2760
cagcguugcaaaucccuuucgccaugcagauggccuacagauucaauggcaucggcguga2820
cccagaaugugcuguacgagaaucagaagcugaucgccaaucaguucaauagcgccaucg2880
gcaagauccaggacagccugagcagcaccgccagcgcccugggcaagcugcaggacgugg2940
ugaaucagaaugcccaggcccugaauacccuggugaagcagcugagcagcaauuucggcg3000
ccaucaguaguguacucaacgacauccugagcagacuggacccgccggaggccgaggugc3060
aaauugaucgucuuauuacuggcagacugcagagccugcagaccuacgugacccagcagc3120
ugaucagagccgccgagaucagagccagcgccaaucuggccgccaccaagaugagcgagu3180
gcgugcugggccagagcaagagaguggacuucugcggcaagggcuaccaccugaugagcu3240
ucccucagagcgcuccacauggcgugguguuccugcacgugaccuacgugccugcccagg3300
agaagaauuucaccaccgcacccgcaaucugccacgacggcaaggcccacuucccuagag3360
agggcguguucgugagcaauggcacccacugguucgugacccagagaaauuucuacgagc3420
cucagaucaucaccaccgacaauaccuucgugagcggcaauugcgacguggugaucggga3480
uagucaauaauacugucuacgacccucugcagccugagcuggacagcuucaaggaggagc3540
uggacaaguacuucaagaaucacaccagcccugacguggaccucggugauauuucgggaa3600
ucaaugccagcguggugaauauccagaaggaaauugaucggcucaacgaaguggccaaga3660
aucugaaugagagccugaucgaccugcaggagcugggcaaguacgagcaguacaucaagu3720
ggccuugguacaucuggcugggcuucaucgccggccugaucgccaucgugauggugacca3780
ucaugcugugcugcaugagcgccaucggcggcuacauccccgaggcccccagggacggcc3840
aggccuacgugaggaaggacggcgagugggugcugcugagcaccuuccugugaagccaac3900
acccugucuaaaaaacauaaauuucuuuaaucauuuugccucuuuucucugugcuucaau3960
uaauaaaaaauggaaagaaccuaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaa4020
aaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaa4080
aaaaaaaaaaaaaaaa4096
<210>47
<211>4039
<212>rna
<213>人工序列
<220>
<223>编码重组s蛋白的mrna
<400>47
gucccgcagucggcguccagcggcucugcuuguucgugugugugucguugcaggccuuau60
ucgccaccauggacuggaucuggagaauccuguuccuggugggggcugccacuggcgccc120
auagccagugcgugaaucugaccaccagaacccagcugccuccugccuacaccaauagcu180
ucaccagaggaguuuauuaucccgauaagguguucagaaguaguguauuacauaguaccc240
aggaccuguuccuaccuuucuucaguaacgugaccugguuccacgccauccacgugagcg300
gcaccaauggcaccaagagauucgacaauccugugcugccuuucaaugacggcguguacu360
ucgccagcaccgagaagagcaauaucaucagaggcuggaucuucggcaccaccuuggauu420
ccaagacucagagccugcugauuguaaacaacgcuacaaauguggugaucaaggugugcg480
aguuccaguucugcaaugacccuuuccuggguguuuauuaucauaagaacaacaagagcu540
ggauggagagcgaguuccgcguauauucgucggcuaauaauugcaccuucgaguacguga600
gccagccuuuccugauggaccuggagggcaagcagggcaauuucaagaaucugagagagu660
ucguguucaagaauaucgacggcuacuucaagaucuacagcaagcacacacccauuaauc720
uggugagagaccugccucagggcuucagcgcccuggagccucugguggaccugccuaucg780
gcaucaauaucaccagauuccagacccugcuggcccugcacagaucauaucuuacaccag840
gcgauucgucaagcgguuggaccgcuggagcugcggcauauuacgugggcuaccugcagc900
cuagaaccuuccugcugaaguacaaugagaaugguacgauaaccgacgcaguugauugug960
cccuggacccucugagcgagaccaagugcacccugaagagcuucaccguggagaagggca1020
ucuaccagaccagcaauuucagagugcagccuaccgagagcaucgugagauucccuaaua1080
ucaccaaucugugcccuuucggcgagguguucaaugccaccagauucgccagcguguacg1140
cauggaaccgcaagcggauaagcaauugcguggccgacuacagcgugcuguacaauagcg1200
ccagcuucagcaccuucaaauguuaugguguuucgccaacaaagcugaaugaccugugcu1260
ucaccaauguguacgccgacagcuucgugaucagaggcgacgaggugagacagaucgcgc1320
cagggcagaccggcaagaucgccgacuacaauuacaagcugccugacgacuucaccggcu1380
gcgugaucgcguggaacucuaacaaccuggacucgaaaguuggaggcaauuacaauuacc1440
uguacagacuguucagaaagagcaaucugaagccuuucgagagagacaucagcaccgaga1500
ucuaccaggccggcagcacaccguguaauggcguggagggcuucaauugcuacuucccuc1560
ugcagagcuacggcuuccagccuaccaauggcgugggcuaccagccuuacagaguggugg1620
ugcugagcuucgagcugcugcacgcucccgcuaccgugugcggcccuaagaagagcacca1680
aucuggugaagaauaagugcgugaauuucaauuucaauggucuaacuggaacgggcgugc1740
ugaccgagagcaauaagaaguuucuucccuuucaacaauucggcagagacaucgccgaca1800
ccacagaugcuguaagagacccucagacccuggagauccuggacaucacuccguguagcu1860
ucggcggcgugagcgugaucacaccgggcaccaauaccagcaaucagguggccgugcugu1920
accaggacgugaauugcaccgaggugccuguggccauccacgccgaccagcugacuccca1980
cuuggaggguauauuccacgggaagcaauguguuccagaccagagccggcugccugaucg2040
gcgccgagcacgugaauaauagcuacgagugcgacaucccuaucggcgccggcaucugcg2100
ccagcuaccagacccagaccaauagcccuggcggcagcggcagcguggccagccagagca2160
ucaucgccuacaccaugagccugggcgccgagaauagcguggccuacagcaauaauagca2220
ucgccaucccuaccaauuucaccaucagcgugaccaccgaaauauuaccagucuccauga2280
ccaagaccagcguggacugcaccauguacaucugcggcgacagcaccgagugcagcaauc2340
ugcugcugcaguacggcagcuucugcacccagcugaauagagcccugaccggcaucgccg2400
uggagcaggacaagaauacccaggagguguucgcccaggugaagcagccagauccaagca2460
agccuagcgccaacagcuucaucgaggaccugcuguucaauaaggugacccuggccgacg2520
ccggcuucaucaagcaguacggcgacugccuaggugauauugcggcaagagaccugaucu2580
gcgcccagaaguuuaacgguuugacaguacuaccuccucugcugaccgacgagaugauag2640
cacaauauacgucggcauugcucgcuggcacgaucacaucgggcuggacuuucggcgccg2700
gagcagcguugcaaaucccuuucgccaugcagauggccuacagauucaauggcaucggcg2760
ugacccagaaugugcuguacgagaaucagaagcugaucgccaaucaguucaauagcgcca2820
ucggcaagauccaggacagccugagcagcaccgccagcgcccugggcaagcugcaggacg2880
uggugaaucagaaugcccaggcccugaauacccuggugaagcagcugagcagcaauuucg2940
gcgccaucaguaguguacucaacgacauccugagcagacuggacccgccggaggccgagg3000
ugcaaauugaucgucuuauuacuggcagacugcagagccugcagaccuacgugacccagc3060
agcugaucagagccgccgagaucagagccagcgccaaucuggccgccaccaagaugagcg3120
agugcgugcugggccagagcaagagaguggacuucugcggcaagggcuaccaccugauga3180
gcuucccucagagcgcuccacauggcgugguguuccugcacgugaccuacgugccugccc3240
aggagaagaauuucaccaccgcacccgcaaucugccacgacggcaaggcccacuucccua3300
gagagggcguguucgugagcaauggcacccacugguucgugacccagagaaauuucuacg3360
agccucagaucaucaccaccgacaauaccuucgugagcggcaauugcgacguggugaucg3420
ggauagucaauaauacugucuacgacccucugcagccugagcuggacagcuucaaggagg3480
agcuggacaaguacuucaagaaucacaccagcccugacguggaccucggugauauuucgg3540
gaaucaaugccagcguggugaauauccagaaggaaauugaucggcucaacgaaguggcca3600
agaaucugaaugagagccugaucgaccugcaggagcugggcaaguacgagcaguacauca3660
aguggccuugguacaucuggcugggcuucaucgccggccugaucgccaucgugaugguga3720
ccaucaugcugugcugcaugagcgccaucggcggcuacauccccgaggcccccagggacg3780
gccaggccuacgugaggaaggacggcgagugggugcugcugagcaccuuccugugaagcc3840
aacacccugucuaaaaaacauaaauuucuuuaaucauuuugccucuuuucucugugcuuc3900
aauuaauaaaaaauggaaagaaccuaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaa3960
aaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaa4020
aaaaaaaaaaaaaaaaaaa4039
<210>48
<211>4154
<212>rna
<213>人工序列
<220>
<223>编码野生型s蛋白的mrna
<400>48
aggagacaagcuugucccgcagucggcguccagcggcucugcuuguucgugugugugucg60
uugcaggccuuauucagaucuaccggugguaccgccaccauguucguguuccuggugcug120
cugccucuggugagcagccagugcgugaaucugaccaccagaacccagcugccuccugcc180
uacaccaauagcuucaccagaggaguuuauuaucccgauaagguguucagaaguagugua240
uuacauaguacccaggaccuguuccuaccuuucuucaguaacgugaccugguuccacgcc300
auccacgugagcggcaccaauggcaccaagagauucgacaauccugugcugccuuucaau360
gacggcguguacuucgccagcaccgagaagagcaauaucaucagaggcuggaucuucggc420
accaccuuggauuccaagacucagagccugcugauuguaaacaacgcuacaaauguggug480
aucaaggugugcgaguuccaguucugcaaugacccuuuccuggguguuuauuaucauaag540
aacaacaagagcuggauggagagcgaguuccgcguauauucgucggcuaauaauugcacc600
uucgaguacgugagccagccuuuccugauggaccuggagggcaagcagggcaauuucaag660
aaucugagagaguucguguucaagaauaucgacggcuacuucaagaucuacagcaagcac720
acacccauuaaucuggugagagaccugccucagggcuucagcgcccuggagccucuggug780
gaccugccuaucggcaucaauaucaccagauuccagacccugcuggcccugcacagauca840
uaucuuacaccaggcgauucgucaagcgguuggaccgcuggagcugcggcauauuacgug900
ggcuaccugcagccuagaaccuuccugcugaaguacaaugagaaugguacgauaaccgac960
gcaguugauugugcccuggacccucugagcgagaccaagugcacccugaagagcuucacc1020
guggagaagggcaucuaccagaccagcaauuucagagugcagccuaccgagagcaucgug1080
agauucccuaauaucaccaaucugugcccuuucggcgagguguucaaugccaccagauuc1140
gccagcguguacgcauggaaccgcaagcggauaagcaauugcguggccgacuacagcgug1200
cuguacaauagcgccagcuucagcaccuucaaauguuaugguguuucgccaacaaagcug1260
aaugaccugugcuucaccaauguguacgccgacagcuucgugaucagaggcgacgaggug1320
agacagaucgcgccagggcagaccggcaagaucgccgacuacaauuacaagcugccugac1380
gacuucaccggcugcgugaucgcguggaacucuaacaaccuggacucgaaaguuggaggc1440
aauuacaauuaccuguacagacuguucagaaagagcaaucugaagccuuucgagagagac1500
aucagcaccgagaucuaccaggccggcagcacaccguguaauggcguggagggcuucaau1560
ugcuacuucccucugcagagcuacggcuuccagccuaccaauggcgugggcuaccagccu1620
uacagagugguggugcugagcuucgagcugcugcacgcucccgcuaccgugugcggcccu1680
aagaagagcaccaaucuggugaagaauaagugcgugaauuucaauuucaauggucuaacu1740
ggaacgggcgugcugaccgagagcaauaagaaguuucuucccuuucaacaauucggcaga1800
gacaucgccgacaccacagaugcuguaagagacccucagacccuggagauccuggacauc1860
acuccguguagcuucggcggcgugagcgugaucacaccgggcaccaauaccagcaaucag1920
guggccgugcuguaccaggacgugaauugcaccgaggugccuguggccauccacgccgac1980
cagcugacucccacuuggaggguauauuccacgggaagcaauguguuccagaccagagcc2040
ggcugccugaucggcgccgagcacgugaauaauagcuacgagugcgacaucccuaucggc2100
gccggcaucugcgccagcuaccagacccagaccaauagcccuagaagagccagaagcgug2160
gccagccagagcaucaucgccuacaccaugagccugggcgccgagaauagcguggccuac2220
agcaauaauagcaucgccaucccuaccaauuucaccaucagcgugaccaccgaaauauua2280
ccagucuccaugaccaagaccagcguggacugcaccauguacaucugcggcgacagcacc2340
gagugcagcaaucugcugcugcaguacggcagcuucugcacccagcugaauagagcccug2400
accggcaucgccguggagcaggacaagaauacccaggagguguucgcccaggugaagcag2460
aucuacaagacuccgccgaucaaggacuucggcggcuucaauuucagccaaauacuccca2520
gauccaagcaagccuagcaagaggagcuucaucgaggaccugcuguucaauaaggugacc2580
cuggccgacgccggcuucaucaagcaguacggcgacugccuaggugauauugcggcaaga2640
gaccugaucugcgcccagaaguuuaacgguuugacaguacuaccuccucugcugaccgac2700
gagaugauagcacaauauacgucggcauugcucgcuggcacgaucacaucgggcuggacu2760
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ggcaucggcgugacccagaaugugcuguacgagaaucagaagcugaucgccaaucaguuc2880
aauagcgccaucggcaagauccaggacagccugagcagcaccgccagcgcccugggcaag2940
cugcaggacguggugaaucagaaugcccaggcccugaauacccuggugaagcagcugagc3000
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gaggccgaggugcaaauugaucgucuuauuacuggcagacugcagagccugcagaccuac3120
gugacccagcagcugaucagagccgccgagaucagagccagcgccaaucuggccgccacc3180
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<210>49
<211>1250
<212>prt
<213>人工序列
<220>
<223>重组sars-cov-2s蛋白
<400>49
metasptrpiletrpargileleupheleuvalglyalaalathrgly
151015
alahisserglncysvalasnleuthrthrargthrglnleupropro
202530
alatyrthrasnserphethrargglyvaltyrtyrproasplysval
354045
pheargserservalleuhisserthrglnaspleupheleuprophe
505560
pheserasnvalthrtrpphehisalailehisvalserglythrasn
65707580
glythrlysargpheaspasnprovalleupropheasnaspglyval
859095
tyrphealaserthrglulysserasnileileargglytrpilephe
100105110
glythrthrleuaspserlysthrglnserleuleuilevalasnasn
115120125
alathrasnvalvalilelysvalcysglupheglnphecysasnasp
130135140
propheleuglyvaltyrtyrhislysasnasnlyssertrpmetglu
145150155160
serglupheargvaltyrserseralaasnasncysthrpheglutyr
165170175
valserglnpropheleumetaspleugluglylysglnglyasnphe
180185190
lysasnleuarggluphevalphelysasnileaspglytyrphelys
195200205
iletyrserlyshisthrproileasnleuvalargaspleuprogln
210215220
glypheseralaleugluproleuvalaspleuproileglyileasn
225230235240
ilethrargpheglnthrleuleualaleuhisargsertyrleuthr
245250255
proglyaspserserserglytrpthralaglyalaalaalatyrtyr
260265270
valglytyrleuglnproargthrpheleuleulystyrasngluasn
275280285
glythrilethraspalavalaspcysalaleuaspproleuserglu
290295300
thrlyscysthrleulysserphethrvalglulysglyiletyrgln
305310315320
thrserasnpheargvalglnprothrgluserilevalargphepro
325330335
asnilethrasnleucyspropheglygluvalpheasnalathrarg
340345350
phealaservaltyralatrpasnarglysargileserasncysval
355360365
alaasptyrservalleutyrasnseralaserpheserthrphelys
370375380
cystyrglyvalserprothrlysleuasnaspleucysphethrasn
385390395400
valtyralaaspserphevalileargglyaspgluvalargglnile
405410415
alaproglyglnthrglylysilealaasptyrasntyrlysleupro
420425430
aspaspphethrglycysvalilealatrpasnserasnasnleuasp
435440445
serlysvalglyglyasntyrasntyrleutyrargleuphearglys
450455460
serasnleulysprophegluargaspileserthrgluiletyrgln
465470475480
alaglyserthrprocysasnglyvalgluglypheasncystyrphe
485490495
proleuglnsertyrglypheglnprothrasnglyvalglytyrgln
500505510
protyrargvalvalvalleuserphegluleuleuhisalaproala
515520525
thrvalcysglyprolyslysserthrasnleuvallysasnlyscys
530535540
valasnpheasnpheasnglyleuthrglythrglyvalleuthrglu
545550555560
serasnlyslyspheleupropheglnglnpheglyargaspileala
565570575
aspthrthraspalavalargaspproglnthrleugluileleuasp
580585590
ilethrprocysserpheglyglyvalservalilethrproglythr
595600605
asnthrserasnglnvalalavalleutyrglnaspvalasncysthr
610615620
gluvalprovalalailehisalaaspglnleuthrprothrtrparg
625630635640
valtyrserthrglyserasnvalpheglnthrargalaglycysleu
645650655
ileglyalagluhisvalasnasnsertyrglucysaspileproile
660665670
glyalaglyilecysalasertyrglnthrglnthrasnserprogly
675680685
glyserglyservalalaserglnserileilealatyrthrmetser
690695700
leuglyalagluasnservalalatyrserasnasnserilealaile
705710715720
prothrasnphethrileservalthrthrgluileleuprovalser
725730735
metthrlysthrservalaspcysthrmettyrilecysglyaspser
740745750
thrglucysserasnleuleuleuglntyrglyserphecysthrgln
755760765
leuasnargalaleuthrglyilealavalgluglnasplysasnthr
770775780
glngluvalphealaglnvallysglniletyrlysthrproproile
785790795800
lysasppheglyglypheasnpheserglnileleuproaspproser
805810815
lysproseralaasnserpheilegluaspleuleupheasnlysval
820825830
thrleualaaspalaglypheilelysglntyrglyaspcysleugly
835840845
aspilealaalaargaspleuilecysalaglnlyspheasnglyleu
850855860
thrvalleuproproleuleuthraspglumetilealaglntyrthr
865870875880
seralaleuleualaglythrilethrserglytrpthrpheglyala
885890895
glyalaalaleuglnileprophealametglnmetalatyrargphe
900905910
asnglyileglyvalthrglnasnvalleutyrgluasnglnlysleu
915920925
ilealaasnglnpheasnseralaileglylysileglnaspserleu
930935940
serserthralaseralaleuglylysleuglnaspvalvalasngln
945950955960
asnalaglnalaleuasnthrleuvallysglnleuserserasnphe
965970975
glyalaileserservalleuasnaspileleuserargleuasppro
980985990
proglualagluvalglnileaspargleuilethrglyargleugln
99510001005
serleuglnthrtyrvalthrglnglnleuileargalaalaglu
101010151020
ileargalaseralaasnleualaalathrlysmetserglucys
102510301035
valleuglyglnserlysargvalaspphecysglylysglytyr
104010451050
hisleumetserpheproglnseralaprohisglyvalvalphe
105510601065
leuhisvalthrtyrvalproalaglnglulysasnphethrthr
107010751080
alaproalailecyshisaspglylysalahispheproargglu
108510901095
glyvalphevalserasnglythrhistrpphevalthrglnarg
110011051110
asnphetyrgluproglnileilethrthraspasnthrpheval
111511201125
serglyasncysaspvalvalileglyilevalasnasnthrval
113011351140
tyraspproleuglnprogluleuaspserphelysglugluleu
114511501155
asplystyrphelysasnhisthrserproaspvalaspleugly
116011651170
aspileserglyileasnalaservalvalasnileglnlysglu
117511801185
ileaspargleuasngluvalalalysasnleuasngluserleu
119011951200
ileaspleuglngluleuglylystyrgluglntyrilelystrp
120512101215
proseralaileglyglytyrileproglualaproargaspgly
122012251230
glnalatyrvalarglysaspglyglutrpvalleuleuserthr
123512401245
pheleu
1250
<210>50
<211>1231
<212>prt
<213>人工序列
<220>
<223>重组sars-cov-2s蛋白
<400>50
metasptrpiletrpargileleupheleuvalglyalaalathrgly
151015
alahisserglncysvalasnleuthrthrargthrglnleupropro
202530
alatyrthrasnserphethrargglyvaltyrtyrproasplysval
354045
pheargserservalleuhisserthrglnaspleupheleuprophe
505560
pheserasnvalthrtrpphehisalailehisvalserglythrasn
65707580
glythrlysargpheaspasnprovalleupropheasnaspglyval
859095
tyrphealaserthrglulysserasnileileargglytrpilephe
100105110
glythrthrleuaspserlysthrglnserleuleuilevalasnasn
115120125
alathrasnvalvalilelysvalcysglupheglnphecysasnasp
130135140
propheleuglyvaltyrtyrhislysasnasnlyssertrpmetglu
145150155160
serglupheargvaltyrserseralaasnasncysthrpheglutyr
165170175
valserglnpropheleumetaspleugluglylysglnglyasnphe
180185190
lysasnleuarggluphevalphelysasnileaspglytyrphelys
195200205
iletyrserlyshisthrproileasnleuvalargaspleuprogln
210215220
glypheseralaleugluproleuvalaspleuproileglyileasn
225230235240
ilethrargpheglnthrleuleualaleuhisargsertyrleuthr
245250255
proglyaspserserserglytrpthralaglyalaalaalatyrtyr
260265270
valglytyrleuglnproargthrpheleuleulystyrasngluasn
275280285
glythrilethraspalavalaspcysalaleuaspproleuserglu
290295300
thrlyscysthrleulysserphethrvalglulysglyiletyrgln
305310315320
thrserasnpheargvalglnprothrgluserilevalargphepro
325330335
asnilethrasnleucyspropheglygluvalpheasnalathrarg
340345350
phealaservaltyralatrpasnarglysargileserasncysval
355360365
alaasptyrservalleutyrasnseralaserpheserthrphelys
370375380
cystyrglyvalserprothrlysleuasnaspleucysphethrasn
385390395400
valtyralaaspserphevalileargglyaspgluvalargglnile
405410415
alaproglyglnthrglylysilealaasptyrasntyrlysleupro
420425430
aspaspphethrglycysvalilealatrpasnserasnasnleuasp
435440445
serlysvalglyglyasntyrasntyrleutyrargleuphearglys
450455460
serasnleulysprophegluargaspileserthrgluiletyrgln
465470475480
alaglyserthrprocysasnglyvalgluglypheasncystyrphe
485490495
proleuglnsertyrglypheglnprothrasnglyvalglytyrgln
500505510
protyrargvalvalvalleuserphegluleuleuhisalaproala
515520525
thrvalcysglyprolyslysserthrasnleuvallysasnlyscys
530535540
valasnpheasnpheasnglyleuthrglythrglyvalleuthrglu
545550555560
serasnlyslyspheleupropheglnglnpheglyargaspileala
565570575
aspthrthraspalavalargaspproglnthrleugluileleuasp
580585590
ilethrprocysserpheglyglyvalservalilethrproglythr
595600605
asnthrserasnglnvalalavalleutyrglnaspvalasncysthr
610615620
gluvalprovalalailehisalaaspglnleuthrprothrtrparg
625630635640
valtyrserthrglyserasnvalpheglnthrargalaglycysleu
645650655
ileglyalagluhisvalasnasnsertyrglucysaspileproile
660665670
glyalaglyilecysalasertyrglnthrglnthrasnserprogly
675680685
glyserglyservalalaserglnserileilealatyrthrmetser
690695700
leuglyalagluasnservalalatyrserasnasnserilealaile
705710715720
prothrasnphethrileservalthrthrgluileleuprovalser
725730735
metthrlysthrservalaspcysthrmettyrilecysglyaspser
740745750
thrglucysserasnleuleuleuglntyrglyserphecysthrgln
755760765
leuasnargalaleuthrglyilealavalgluglnasplysasnthr
770775780
glngluvalphealaglnvallysglnproaspproserlysproser
785790795800
alaasnserpheilegluaspleuleupheasnlysvalthrleuala
805810815
aspalaglypheilelysglntyrglyaspcysleuglyaspileala
820825830
alaargaspleuilecysalaglnlyspheasnglyleuthrvalleu
835840845
proproleuleuthraspglumetilealaglntyrthrseralaleu
850855860
leualaglythrilethrserglytrpthrpheglyalaglyalaala
865870875880
leuglnileprophealametglnmetalatyrargpheasnglyile
885890895
glyvalthrglnasnvalleutyrgluasnglnlysleuilealaasn
900905910
glnpheasnseralaileglylysileglnaspserleuserserthr
915920925
alaseralaleuglylysleuglnaspvalvalasnglnasnalagln
930935940
alaleuasnthrleuvallysglnleuserserasnpheglyalaile
945950955960
serservalleuasnaspileleuserargleuaspproprogluala
965970975
gluvalglnileaspargleuilethrglyargleuglnserleugln
980985990
thrtyrvalthrglnglnleuileargalaalagluileargalaser
99510001005
alaasnleualaalathrlysmetserglucysvalleuglygln
101010151020
serlysargvalaspphecysglylysglytyrhisleumetser
102510301035
pheproglnseralaprohisglyvalvalpheleuhisvalthr
104010451050
tyrvalproalaglnglulysasnphethrthralaproalaile
105510601065
cyshisaspglylysalahispheproarggluglyvalpheval
107010751080
serasnglythrhistrpphevalthrglnargasnphetyrglu
108510901095
proglnileilethrthraspasnthrphevalserglyasncys
110011051110
aspvalvalileglyilevalasnasnthrvaltyraspproleu
111511201125
glnprogluleuaspserphelysglugluleuasplystyrphe
113011351140
lysasnhisthrserproaspvalaspleuglyaspilesergly
114511501155
ileasnalaservalvalasnileglnlysgluileaspargleu
116011651170
asngluvalalalysasnleuasngluserleuileaspleugln
117511801185
gluleuglylystyrgluglntyrilelystrpproseralaile
119011951200
glyglytyrileproglualaproargaspglyglnalatyrval
120512101215
arglysaspglyglutrpvalleuleuserthrpheleu
122012251230
<210>51
<211>1274
<212>prt
<213>人工序列
<220>
<223>重组sars-cov-2s蛋白
<400>51
metasptrpiletrpargileleupheleuvalglyalaalathrgly
151015
alahisserglncysvalasnleuthrthrargthrglnleupropro
202530
alatyrthrasnserphethrargglyvaltyrtyrproasplysval
354045
pheargserservalleuhisserthrglnaspleupheleuprophe
505560
pheserasnvalthrtrpphehisalailehisvalserglythrasn
65707580
glythrlysargpheaspasnprovalleupropheasnaspglyval
859095
tyrphealaserthrglulysserasnileileargglytrpilephe
100105110
glythrthrleuaspserlysthrglnserleuleuilevalasnasn
115120125
alathrasnvalvalilelysvalcysglupheglnphecysasnasp
130135140
propheleuglyvaltyrtyrhislysasnasnlyssertrpmetglu
145150155160
serglupheargvaltyrserseralaasnasncysthrpheglutyr
165170175
valserglnpropheleumetaspleugluglylysglnglyasnphe
180185190
lysasnleuarggluphevalphelysasnileaspglytyrphelys
195200205
iletyrserlyshisthrproileasnleuvalargaspleuprogln
210215220
glypheseralaleugluproleuvalaspleuproileglyileasn
225230235240
ilethrargpheglnthrleuleualaleuhisargsertyrleuthr
245250255
proglyaspserserserglytrpthralaglyalaalaalatyrtyr
260265270
valglytyrleuglnproargthrpheleuleulystyrasngluasn
275280285
glythrilethraspalavalaspcysalaleuaspproleuserglu
290295300
thrlyscysthrleulysserphethrvalglulysglyiletyrgln
305310315320
thrserasnpheargvalglnprothrgluserilevalargphepro
325330335
asnilethrasnleucyspropheglygluvalpheasnalathrarg
340345350
phealaservaltyralatrpasnarglysargileserasncysval
355360365
alaasptyrservalleutyrasnseralaserpheserthrphelys
370375380
cystyrglyvalserprothrlysleuasnaspleucysphethrasn
385390395400
valtyralaaspserphevalileargglyaspgluvalargglnile
405410415
alaproglyglnthrglylysilealaasptyrasntyrlysleupro
420425430
aspaspphethrglycysvalilealatrpasnserasnasnleuasp
435440445
serlysvalglyglyasntyrasntyrleutyrargleuphearglys
450455460
serasnleulysprophegluargaspileserthrgluiletyrgln
465470475480
alaglyserthrprocysasnglyvalgluglypheasncystyrphe
485490495
proleuglnsertyrglypheglnprothrasnglyvalglytyrgln
500505510
protyrargvalvalvalleuserphegluleuleuhisalaproala
515520525
thrvalcysglyprolyslysserthrasnleuvallysasnlyscys
530535540
valasnpheasnpheasnglyleuthrglythrglyvalleuthrglu
545550555560
serasnlyslyspheleupropheglnglnpheglyargaspileala
565570575
aspthrthraspalavalargaspproglnthrleugluileleuasp
580585590
ilethrprocysserpheglyglyvalservalilethrproglythr
595600605
asnthrserasnglnvalalavalleutyrglnaspvalasncysthr
610615620
gluvalprovalalailehisalaaspglnleuthrprothrtrparg
625630635640
valtyrserthrglyserasnvalpheglnthrargalaglycysleu
645650655
ileglyalagluhisvalasnasnsertyrglucysaspileproile
660665670
glyalaglyilecysalasertyrglnthrglnthrasnserprogly
675680685
glyserglyservalalaserglnserileilealatyrthrmetser
690695700
leuglyalagluasnservalalatyrserasnasnserilealaile
705710715720
prothrasnphethrileservalthrthrgluileleuprovalser
725730735
metthrlysthrservalaspcysthrmettyrilecysglyaspser
740745750
thrglucysserasnleuleuleuglntyrglyserphecysthrgln
755760765
leuasnargalaleuthrglyilealavalgluglnasplysasnthr
770775780
glngluvalphealaglnvallysglniletyrlysthrproproile
785790795800
lysasppheglyglypheasnpheserglnileleuproaspproser
805810815
lysproseralaasnserpheilegluaspleuleupheasnlysval
820825830
thrleualaaspalaglypheilelysglntyrglyaspcysleugly
835840845
aspilealaalaargaspleuilecysalaglnlyspheasnglyleu
850855860
thrvalleuproproleuleuthraspglumetilealaglntyrthr
865870875880
seralaleuleualaglythrilethrserglytrpthrpheglyala
885890895
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1.重组sars-cov-2刺突蛋白(s蛋白)或其抗原性片段，所述s蛋白或抗原性片段在氨基酸位置817-987处具有一个或多个氨基酸残基突变为脯氨酸的突变，其中根据seqidno.1所示的氨基酸序列编号。
2.根据权利要求1的重组s蛋白或其抗原性片段，其中所述突变包括k986p和/或v987p取代，优选k986p和v987p取代两者。
3.根据权利要求1或2的重组s蛋白或其抗原性片段，其不包含功能性融合肽结构域，例如不包含融合肽结构域。
4.根据权利要求1-3中任一项的重组s蛋白或其抗原性片段，其中s1/s2切割位点rrar和/或s2切割位点kr经突变以失去被弗林样蛋白酶和溶酶体蛋白酶切割的能力。
5.权利要求4的重组s蛋白或其抗原性片段，其中所述s1/s2切割位点突变为ggsg和/或所述s2切割位点突变为an。
6.根据权利要求1-5中任一项的重组s蛋白或其抗原性片段，其还包含三聚体结构域，所述三聚体结构域当表达时促进所述重组s蛋白或其抗原性片段形成三聚体。
7.权利要求6的重组s蛋白或其抗原性片段，其中所述三聚体结构域包含t4噬菌体fibritin三聚体基序，优选具有seqidno.40所示的氨基酸序列。
8.权利要求6或7的重组s蛋白或其抗原性片段，其中所述三聚体结构域通过任选的接头序列融合在所述重组s蛋白或其抗原性片段的c端。
9.权利要求8的重组s蛋白或其抗原性片段，其中所述接头序列包含saig。
10.权利要求1-9中任一项的重组s蛋白或其抗原性片段，其还包含信号序列。
11.权利要求10的重组s蛋白或其抗原性片段，其中所述信号序列包含免疫球蛋白重链可变区(igvh)信号序列，优选具有seqidno.39所示的氨基酸序列。
12.重组sars-cov-2刺突蛋白(s蛋白)或其抗原性片段，其具有选自seqidno.3-38和seqidno.49-52中任一项所示的氨基酸序列。
13.权利要求12的重组s蛋白或其抗原性片段，其具有选自seqidno.29、seqidno.32、seqidno.35、seqidno.38和seqidno.49-52中任一项所示的氨基酸序列。
14.mrna，所述mrna编码权利要求1-13中任一项所述的重组s蛋白或其抗原性片段。
15.权利要求14的mrna，其从上游到下游包含帽结构、5’-utr、开放阅读框(orf)、3’-utr和poly-a尾。
16.权利要求15的mrna，其中所述帽结构具有m7g5’ppp5’(2’-ome)npg，其中m7g为n7-甲基鸟嘌呤核苷；p为磷酸；ppp为三磷酸；2’-ome为2’-甲氧基修饰；n为任意核苷。
17.权利要求15或16的mrna，其中所述5’-utr包含选自下组的基因的5’-utr或其同源物、片段或变体：β-珠蛋白基因、热休克蛋白70基因、轴丝动力蛋白重链2基因、羟基类固醇(17-β)脱氢酶基因，和/或kozak序列。
18.权利要求17的mrna，其中所述5’-utr包含seqidno.41或seqidno.42，或seqidno:41和seqidno:42的组合。
19.权利要求15-18中任一项的mrna，其中所述3’-utr包含选自下组的基因的3’-utr或其同源物、片段或变体：白蛋白基因、α-珠蛋白基因、β-珠蛋白基因、酪氨酸羟化酶基因、热休克蛋白70基因，脂氧合酶基因和胶原蛋白α基因。
20.权利要求19的mrna，其中所述3’-utr包含seqidno.43。
21.权利要求15-20中任一项所述的mrna，其中所述polya尾的长度为100-200个核苷酸，例如约120个核苷酸。
22.mrna，其包含选自seqidno.44-47中任一项所示的序列。
23.权利要求14-22中任一项的mrna，其中所述mrna中的一个或多个核苷酸是经修饰的。
24.权利要求23的mrna，其中所述mrna中的一个或多个核苷酸各自独立替换为天然存在的核苷酸类似物或人工合成的核苷酸类似物。
25.权利要求24的mrna，其中所述天然存在的核苷酸类似物选自假尿苷(pseudouridine)、2-硫尿苷(2-thiouridine)、5-甲基尿苷(5-methyluridine)，5-甲基胞苷(5-methylcytidine)和n6-甲基腺苷(n6-methyladenosine)，和所述人工合成的核苷酸类似物选自n1-甲基假尿苷(n1-methylpseudouridine)和5-乙炔基尿苷(5-ethynyluridine)。
26.权利要求25的mrna，其中所述mrna中的一个或多个尿苷三磷酸(utp)各自独立替换为假尿苷三磷酸(pseudo-utp)、1-甲基-假尿苷三磷酸(n1-methyl-pseudo-utp)或5-乙炔基尿苷三磷酸(5-ethynyl-utp)，和/或一个或多个胞苷三磷酸(ctp)替换为5-甲基胞苷三磷酸(5-methyl-ctp)。
27.核酸分子，其编码权利要求14-22中任一项所述的mrna。
28.mrna-载体颗粒，其包含权利要求14-26中任一项的mrna和包封所述mrna的载体材料。
29.权利要求28的mrna-载体颗粒，其中所述载体材料选自鱼精蛋白、脂质纳米颗粒(lnp)、高分子材料和无机纳米颗粒。
30.权利要求29的mrna-载体颗粒，其中所述载体材料是lnp。
31.权利要求30的mrna-载体颗粒，其中所述lnp包含离子化脂质、聚乙二醇化脂质、胆固醇及衍生物和磷脂中的一种或多种。
32.权利要求31的mrna-载体颗粒，其中所述lnp包含离子化脂质、聚乙二醇化脂质、胆固醇及衍生物和磷脂。
33.组合物，其包含一种或多种如权利要求1-13中任一项定义的重组s蛋白或其抗原性片段、权利要求14-26中任一项的mrna或权利要求28-32中任一项的mrna-载体颗粒。
34.权利要求33的组合物，其中所述组合物是疫苗组合物，任选所述疫苗组合物还包含一种或多种佐剂。
35.权利要求1-13中任一项定义的重组s蛋白或其抗原性片段、权利要求14-26中任一项的mrna、权利要求28-32中任一项的mrna-载体颗粒或权利要求33或34的组合物在制备用于在受试者中预防和/或治疗与sars-cov-2感染相关的疾病或病症的药物中的用途。
36.制备mrna疫苗的方法，所述方法包括：
a.将离子化脂质、聚乙二醇化脂质、胆固醇及衍生物和磷脂溶解在乙醇溶液中；
b.通过微流控混合器将所述脂质乙醇溶液与mrna水溶液混合得到脂质纳米颗粒(lnp)；
c.分离和纯化步骤b中获得的lnp从而获得所述mrna疫苗，
其中所述mrna如权利要求14-26中任一项定义。
37.权利要求36的方法，其中步骤c包括对所述lnp进行透析、超滤和微米膜过滤。
技术总结